@article{mbs:/content/journal/jgv/10.1099/0022-1317-66-7-1601, author = "Rosenthal, Ken S. and Perez, Ray and Hodnichak, Cheryl", title = "Inhibition of Herpes Simplex Virus Type 1 Penetration by Cytochalasins B and D", journal= "Journal of General Virology", year = "1985", volume = "66", number = "7", pages = "1601-1605", doi = "https://doi.org/10.1099/0022-1317-66-7-1601", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-7-1601", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "cytochalasins", keywords = "HSV-1", keywords = "penetration", abstract = "SUMMARY Internalization of herpes simplex virus type 1 (HSV) KOS strain by HEp-2 cells was reversibly inhibited by pretreatment of cells with cytochalasins B and D. Internalization of virus following preincubation at 4 °C and temperature shift to 37 °C was normally preceded by a 5 to 8 min lag period and was complete within 20 to 30 min. A similar lag period followed HSV addition at 37 °C. Cytochalasin D was fivefold more active on HSV entry than cytochalasin B, with 50% inhibition at 2 µm and 10 µm respectively. Inhibition was completely reversible, such that all cell-bound infectious virus was recovered upon removal of cytochalasin. In conjunction with previous reports, the activity of cytochalasin on HSV entry suggests that a change in cytoskeletal structure following virus attachment triggers a microfilament activity important for internalization of HSV by HEp-2 cells.", }