1887

Abstract

SUMMARY

Internalization of herpes simplex virus type 1 (HSV) KOS strain by HEp-2 cells was reversibly inhibited by pretreatment of cells with cytochalasins B and D. Internalization of virus following preincubation at 4 °C and temperature shift to 37 °C was normally preceded by a 5 to 8 min lag period and was complete within 20 to 30 min. A similar lag period followed HSV addition at 37 °C. Cytochalasin D was fivefold more active on HSV entry than cytochalasin B, with 50% inhibition at 2 µ and 10 µ respectively. Inhibition was completely reversible, such that all cell-bound infectious virus was recovered upon removal of cytochalasin. In conjunction with previous reports, the activity of cytochalasin on HSV entry suggests that a change in cytoskeletal structure following virus attachment triggers a microfilament activity important for internalization of HSV by HEp-2 cells.

Keyword(s): cytochalasins , HSV-1 and penetration
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1601
1985-07-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071601.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1601&mimeType=html&fmt=ahah

References

  1. Asano A., Okada Y. 1977; Inhibition of virus-induced fusion of Ehrlich ascites tumor cells by cytochalasin B and D. Life Sciences 20:117–122
    [Google Scholar]
  2. Coombs K., Mann E., Edwards J., Brown D. T. 1981; Effects of chloroquine and cytochalasin B on the infection of cells by Sindbis virus and vesicular stomatitis virus. Journal of Virology 37:1060–1065
    [Google Scholar]
  3. Deluca N., Bzik D., Person S., Snipes W. 1981; Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions. Proceedings of the National Academy of Sciences, U.S.A 78:912–916
    [Google Scholar]
  4. Dix R. D., Courtney R. J. 1976; Effects of cytochalasin B on HSV type 1 replication. Virology 70:127–135
    [Google Scholar]
  5. Dreesman G. R., Benyesh-Melnick M. 1967; Spectrum of human cytomegalovirus complement-fixing antigens. Journal of Immunology 99:1106–1114
    [Google Scholar]
  6. Epstein R. L., Powers M. L., Weiner H. L. 1983; Interaction of reovirus with cell surface receptors. III. Reovirus type 3 induces capping of viral receptors on murine lymphocytes. Journal of Immunology 127:1800–1803
    [Google Scholar]
  7. Farber F. E., Eberle R. 1976; Effects of cytochalasin and alkaloid drugs on the biological expression of HSV2 DNA. Experimental Cell Research 103:15–22
    [Google Scholar]
  8. Gall W. E., Edelman G. M. 1981; Lateral diffusion of surface molecules in animal cells and tissues. Science 213:903–905
    [Google Scholar]
  9. Huang A. S., Wagner R. R. 1964; Penetration of HSV into human epidermoid cells. Proceedings of the Society for Experimental Biology and Medicine 116:863–869
    [Google Scholar]
  10. Lycke E., Kristensson K., Svennerholm B., Vahlne A., Ziegler R. 1984; Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture. Journal of General Virology 65:55–64
    [Google Scholar]
  11. Maclean-Fletcher S., Pollard T. D. 1980; Mechanism of action of cytochalasin B on actin. Cell 20:329–341
    [Google Scholar]
  12. Miyake Y., Kim J., Okada Y. 1978; Effects of cytochalasin D on fusion of cells by HVJ (Sendai virus). Experimental Cell Research 116:167–178
    [Google Scholar]
  13. Nemerow G. R., Cooper N. R. 1984; Early events in the infection of human B lymphocytes by Epstein-Barr virus: the internalization process. Virology 132:186–198
    [Google Scholar]
  14. Norrild B., Virtanen I., Lehto V.-P., Pedersen B. 1983; Accumulation of herpes simplex virus type 1 glycoprotein D in adhesion areas of infected cells. Journal of General Virology 64:2499–2503
    [Google Scholar]
  15. Patterson S., Russell W. C. 1983; Ultrastructural and immunofluorescence studies of early events in adenovirus-HeLa cell interactions. Journal of General Virology 64:1091–1099
    [Google Scholar]
  16. Patterson S., Oxford J. S., Dourmashkin R. R. 1979; Studies on the mechanism of influenza virus entry into cells. Journal of General Virology 43:223–229
    [Google Scholar]
  17. Rosenthal K. S., Leuther M. B., Barisas B. G. 1984; HSV binding and entry modulates cell surface protein mobility. Journal of Virology 49:980–983
    [Google Scholar]
  18. Sarmiento M., Haffey M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. Ⅲ. Role of glycoprotein VP7 (B2) in virion infectivity. Journal of Virology 29:1149–1158
    [Google Scholar]
  19. Tanenbaum J. 1978; Approaches to the molecular biology of cytochalasin action. In Cytochalasins-Biochemical and Cell Biological Aspects pp 521–559 Edited by Tanenbaum S. W. New York: Elsevier;
    [Google Scholar]
  20. Yahara I., Edelman G. M. 1975; Electron microscopic analysis of the modulation of lymphocyte receptor mobility. Experimental Cell Research 91:125–142
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1601
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1601
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error