1887

Abstract

SUMMARY

A deletion mutant derived from herpes simplex virus type 1 (HSV-1) strain ANG was analysed. The deletion mapped within the polypeptide-coding region of the immediate-early ICP4 gene. Based on DNA sequence data the deletion was shown to comprise 84 base pairs. In the wild-type genome of strain ANG these sequences were almost completely homologous to the known sequences of HSV-1 strain 17. The ICP4 polypeptide induced by the mutant was similar in size to the wild-type ICP4 protein and was recognized by a monoclonal antibody against ICP4. The data presented suggest that the deletion corresponds to a region on the ICP4 polypeptide that is nonessential for the replication of the virus .

Keyword(s): deletion mutant , HSV-1 and ICP4
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1589
1985-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071589.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1589&mimeType=html&fmt=ahah

References

  1. Clements J. B., Mclauchlan J., Mcgeoch D. J. 1979; Orientation of herpes simplex virus type 1 immediate early mRNA’s. Nucleic Acids Research 7:77–91
    [Google Scholar]
  2. Courtney R. J., Benyesh-Melnick M. 1974; Isolation and characterization of a large molecular weight polypeptide of herpes simplex virus type 1. Virology 73:286–294
    [Google Scholar]
  3. Davison M.-J., Preston V. G., Mcgeoch D. J. 1984; Determination of the sequence alteration in the DNA of herpes simplex virus type 1 temperature-sensitive mutant tsK. Journal of General Virology 65:859–863
    [Google Scholar]
  4. Deluca N. L., Courtney M. A., Schaffer P. A. 1984; Temperature-sensitive mutants in herpes simplex virus type 1 ICP4 permissive for early gene expression. Journal of Virology 52:767–776
    [Google Scholar]
  5. Dixon R. A. F., Schaffer R. A. 1980; Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP 175. Journal of Virology 36:189–203
    [Google Scholar]
  6. Honess R. W., Roizman B. 1974; Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
    [Google Scholar]
  7. Kaerner H. C., Schrӧder C. H., Ott-Hartmann A., Kümel G., Kirchner H. 1983; Genetic variability of herpes simplex virus. I. Development of a pathogenic variant during passaging of a nonpathogenic HSV-1 virus strain in mouse brain. Journal of Virology 46:83–93
    [Google Scholar]
  8. Knipe D. M., Ruyechan W. T., Roizman B., Halliburton I. W. 1978; Molecular genetics of herpes simplex virus: demonstration of regions of obligatory and non-obligatory identity within diploid regions of the genome by sequence replacement and insertion. Proceedings of the National Academy of Sciences, U.S.A 75:3896–3900
    [Google Scholar]
  9. Lonsdale D. M., Brown S. M., Lang J., Subak-Sharpe J. H., Koprowski H., Warren K. G. 1980; Variations in herpes simplex virus from human ganglia and study of clonal variation in HSV-1. Annals of the New York Academy of Sciences 354:291–308
    [Google Scholar]
  10. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  11. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  12. Murchie M.-J., Mcgeoch D. J. 1982; DNA sequence analysis of an immediate-early gene region of the herpes simplex virus type 1 genome (map coordinates 0-950 to 0-978). Journal of General Virology 62:1–15
    [Google Scholar]
  13. Parris D. S., Courtney R. J., Schaffer P. A. 1978; Temperature-sensitive mutants of herpes simplex virus type 1 defective in transcriptional and post-transcriptional functions required for viral DNA synthesis. Virology 90:177–186
    [Google Scholar]
  14. Preston C. M. 1979; Control of herpes simplex virus type 1 mRN A synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK. Journal of Virology 24:275–284
    [Google Scholar]
  15. Preston V. G. 1981; Fine-structure mapping of herpes simplex virus type 1 temperature-sensitive mutations within the short repeat region of the genome. Journal of Virology 39:150–161
    [Google Scholar]
  16. Rixon F. J., Campbell M. E., Clements J. B. 1982; The immediate-early mRNA that encodes the regulatory polypeptide Vmv175 of herpes simplex virus type 1 is unspliced. EMBO Journal 1:1223–1277
    [Google Scholar]
  17. Schaffer P. A., Carter V. C., Timbury M. C. 1978; Collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2. Journal of Virology 27:490–504
    [Google Scholar]
  18. Showalter S. D., Zweig M., Hampar B. 1982; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP4. Infection and Immunity 34:684–692
    [Google Scholar]
  19. Thomas J. O., Kornberg R. D. 1975; An octamer of histones in chromatin and free in solution. Proceedings of the National Academy of Sciences, U.S.A 72:2626–2630
    [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  21. Watson R. J., Clements J. B. 1978; Characterization of transcription-deficient temperature-sensitive mutants of herpes simplex virus type 1. Virology 91:364–379
    [Google Scholar]
  22. Wilkie N. M., Davison A., Chartrand P., Stow N. D., Preston V. G., Timbury M. C. 1978; Recombination in herpes simplex virus: mapping of mutations and analysis of intertypic recombinants. Cold Spring Harbor Symposia on Quantitative Biology 43:827–840
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1589
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1589
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error