1887

Abstract

SUMMARY

Particles of carrot red leaf virus (CRLV; luteovirus group) purified from chervil () contain a single ssRNA species of mol. wt. about 1.8 × 10 and a major protein of mol. wt. about 25000. CRLV acts as a helper for aphid transmission of carrot mottle virus (CMotV; ungrouped) from mixedly infected plants. Virus preparations purified from such plants possess the infectivity of both viruses but contain particles indistinguishable from those of CRLV; some of the particles are therefore thought to consist of CMotV RNA packaged in CRLV coat protein. When RNA from such preparations was electrophoresed in agarose/polyacrylamide gels, CMotV infectivity was associated with an RNA band that migrated ahead of the CRLV RNA band and had an estimated mol. wt. of about 1.5 × 10, similar to that previously found for the infective ssRNA extracted directly from leaves infected with CMotV alone. Preparations of dsRNA from CMotV-infected leaves contained two species: one of mol. wt. about 3.2 × 10, presumably the replicative form of the infective ssRNA, and the other, mol. wt. about 0.9 × 10, of unknown origin and function. The infective agent in buffer extracts of CMotV-infected was resistant to RNase (although the enzyme acted as a reversible inhibitor of infection at high concentrations) and is therefore not unprotected RNA. It may be protected within the approximately 52 nm enveloped structures previously reported.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1575
1985-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071575.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1575&mimeType=html&fmt=ahah

References

  1. Bawden F. C., Kleczkowski A. 1948; Variations in the properties of potato virus × and their effects on its interactions with ribonuclease and proteolytic enzymes. Journal of General Microbiology 2:173–185
    [Google Scholar]
  2. Bem F., Murant A. F. 1979; Comparison of particle properties of heracleum latent and apple chlorotic leaf spot viruses. Journal of General Virology 44:817–826
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller Ft. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A 75:4801–4805
    [Google Scholar]
  4. Brosius J., Dull T. J., Noller H. F. 1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A 77:201–204
    [Google Scholar]
  5. Carbon P., Ehresmann C., Ehresmann B., Ebel J. P. 1978; The sequence of Escherichia coli 16S RNA determined by new rapid gel methods. FEBS Letters 94:152–156
    [Google Scholar]
  6. Dodds J. A., Bar-Joseph M. 1983; Double-stranded RNA from plants infected with closteroviruses. Phytopathology 73:419–423
    [Google Scholar]
  7. Elnagar S., Murant A. F. 1978; Relations of carrot red leaf and carrot mottle viruses with their aphid vector, Cavariella aegopodii. Annals of Applied Biology 89:237–244
    [Google Scholar]
  8. Falk B. W., Morris T. J., Duffus J. E. 1979; Unstable infectivity and sedimentable ds-RNA associated with lettuce speckles mottle virus. Virology 96:239–248
    [Google Scholar]
  9. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A 79:5818–5822
    [Google Scholar]
  10. Halk E. L., Robinson D. J., Murant A. F. 1979; Molecular weight of the infective RNA from leaves infected with carrot mottle virus. Journal of General Virology 45:383–388
    [Google Scholar]
  11. Igloi G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
    [Google Scholar]
  12. Loring H. S. 1942; The reversible inactivation of tobacco mosaic virus by crystalline ribonuclease. Journal of General Physiology 25:497–505
    [Google Scholar]
  13. Murant A. F., Goold R. A., Roberts I. M., Cathro J. 1969; Carrot mottle-a persistent aphid-borne virus with unusual properties and particles. Journal of General Virology 4:329–341
    [Google Scholar]
  14. Reddy D. V. R., Black L. M. 1973; Electrophoretic separation of all components of the double-stranded RNA of wound tumor virus. Virology 54:557–562
    [Google Scholar]
  15. Reddy D. V. R., Kimura I., Black L. M. 1974; Co-electrophoresis of dsRNA from wound tumor and rice dwarf viruses. Virology 60:293–296
    [Google Scholar]
  16. Reddy D. V. R., Boccardo G., Outridge R., Teakle D. S., Black L. M. 1975; Electrophoretic separation of dsRNA genome segments from Fiji disease and maize rought dwarf viruses. Virology 63:287–291
    [Google Scholar]
  17. Waterhouse P. M., Murant A. F. 1981; Purification of carrot red leaf virus and evidence from four serological tests for its relationship to luteoviruses. Annals of Applied Biology 97:191–204
    [Google Scholar]
  18. Waterhouse P. M., Murant A. F. 1983; Further evidence on the nature of the dependence of carrot mottle virus on carrot red leaf virus for transmission by aphids. Annals of Applied Biology 103:455–464
    [Google Scholar]
  19. Watson M., Serjeant E. P., Lennon E. A. 1964; Carrot motley dwarf and parsnip mottle viruses. Annals of Applied Biology 54:153–166
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1575
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1575
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error