1887

Abstract

SUMMARY

Particles of carrot red leaf virus (CRLV; luteovirus group) purified from chervil () contain a single ssRNA species of mol. wt. about 1.8 × 10 and a major protein of mol. wt. about 25000. CRLV acts as a helper for aphid transmission of carrot mottle virus (CMotV; ungrouped) from mixedly infected plants. Virus preparations purified from such plants possess the infectivity of both viruses but contain particles indistinguishable from those of CRLV; some of the particles are therefore thought to consist of CMotV RNA packaged in CRLV coat protein. When RNA from such preparations was electrophoresed in agarose/polyacrylamide gels, CMotV infectivity was associated with an RNA band that migrated ahead of the CRLV RNA band and had an estimated mol. wt. of about 1.5 × 10, similar to that previously found for the infective ssRNA extracted directly from leaves infected with CMotV alone. Preparations of dsRNA from CMotV-infected leaves contained two species: one of mol. wt. about 3.2 × 10, presumably the replicative form of the infective ssRNA, and the other, mol. wt. about 0.9 × 10, of unknown origin and function. The infective agent in buffer extracts of CMotV-infected was resistant to RNase (although the enzyme acted as a reversible inhibitor of infection at high concentrations) and is therefore not unprotected RNA. It may be protected within the approximately 52 nm enveloped structures previously reported.

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1985-07-01
2022-01-23
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