1887

Abstract

SUMMARY

The nucleotide sequences of cDNA clones corresponding to RNA-3 and RNA-4 of beet necrotic yellow vein virus isolates F2 and G1 have been determined. The cDNA of RNA-3 of isolate F2 is 1775 residues in length and contains a coding region of 219 codons. In isolate G1 this coding region has undergone an internal deletion of 354 nucleotides in such a way as to conserve a shortened reading frame. Otherwise, the RNA-3 sequences of the two isolates were closely similar. RNA-4 of isolate F2 has an extrapolated length of 1431 residues and contains an open reading frame of 282 codons. This open reading frame has undergone an internal deletion of 324 nucleotides in one cDNA clone of RNA-4(G1) with conservation of a shortened reading frame. Sequence analysis of other RNA-4(G1) cDNA clones revealed, however, that the exact boundaries of the deletion are not always the same. RNA-3 and RNA-4 of each isolate are more than 90% homologous for the 3′-terminal 200 nucleotides. Short homologous sequences are also present in RNA-3 and RNA-4 of isolate F2 flanking the regions deleted in each of these RNAs in the G1 isolate. These homologous sequences probably play a role in the deletion process.

Keyword(s): cDNA cloning , deletion and satellite RNA
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1553
1985-07-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071553.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1553&mimeType=html&fmt=ahah

References

  1. Alwine J. C., Kemp D. J., Stark G. R. 1977; Method of detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probe. Proceedings of the National Academy of Sciences, U.S.A 74:5350–5354
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant DNA plasmids. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Cornelissen B., Brederode F., Moormann R., Bol J. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA 1. Nucleic Acids Research 11:1253–1265
    [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  5. Fields S., Winter G. 1981; Nucleotide sequence heterogeneity and sequence rearrangements in influenza virus cDNA. Gene 15:207–214
    [Google Scholar]
  6. Franck A., Guilley H., Jonard G., Richards K., Hirth L. 1980; Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–294
    [Google Scholar]
  7. Garcia-arenal F., Palukaitis P., Zaitlin M. 1984; Strains and mutants of tobacco mosaic virus are both found in virus derived from single-lesion-passaged inoculum. Virology 132:131–137
    [Google Scholar]
  8. Gould A., Symons R. H. 1982; Cucumber mosaic virus RNA 3: determination of the nucleotide sequence provides the amino acid sequences of protein 3a and viral coat protein. European Journal of Biochemistry 126:217–226
    [Google Scholar]
  9. Guilley H., Dudley R. K., Jonard G., Balazs E., Richards K. 1982; Transcription of cauliflower mosaic virus DNA: detection of promoter sequences and characterization of transcripts. Cell 30:763–773
    [Google Scholar]
  10. Haseloff J., Symons R. H. 1982; Comparative sequence and structure of viroid-like RNAs of two plant viruses. Nucleic Acids Research 10:3681–3691
    [Google Scholar]
  11. Heidecker G., Messing J. 1983; Sequence analysis of zein cDNAs obtained by an efficient mRNA cloning method. Nucleic Acids Research 11:4891–4906
    [Google Scholar]
  12. Maizel J., Lenk R. 1981; Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proceedings of the National Academy of Sciences, U.S.A 78:7665–7669
    [Google Scholar]
  13. Mandel M., Higa A. 1970; Calcium dependent bacteriophage DNA infection. Journal of Molecular Biology 11:159–162
    [Google Scholar]
  14. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences, U.S.A 74:560–564
    [Google Scholar]
  15. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–77
    [Google Scholar]
  16. Mount S. M. 1982; A catalogue of splice junction sequences. Nucleic Acids Research 10:459–472
    [Google Scholar]
  17. Putz C., Pinck L., Pinck M., Fritsch C. 1983; Identification of the 3′ and 5′ ends of beet necrotic yellow vein virus RNAs. Presence of poly A sequences. FEBS Letters 156:41–56
    [Google Scholar]
  18. Richards K., Jonard G., Guilley H., Ziegler V., Putz C. 1985; In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes. Journal of General Virology 66:345–350
    [Google Scholar]
  19. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  20. Shirako Y., Brakke M. K. 1984; Spontaneous deletion mutation of soil-borne wheat mosaic virus RNA II. Journal of General Virology 65:855–858
    [Google Scholar]
  21. Tamada T. 1975; Beet necrotic yellow vein virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No. 144
    [Google Scholar]
  22. Tamada T., Baba T. 1973; Beet necrotic yellow vein virus from rhizomania-affected sugar beet in Japan. Annals of the Phytopathological Society of Japan 39:325–332
    [Google Scholar]
  23. van Wezenbeek P., Verver J., Harmsen J., Vos P., van Kammen A. 1983; Primary structure and gene organization of the middle component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
    [Google Scholar]
  24. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  25. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences, U.S.A 76:615–619
    [Google Scholar]
  26. Volckaert G., Tavernier J., Derynck R., Devos R., Fiers W. 1981; Molecular mechanisms of nucleotide-sequence rearrangements in cDNA clones of human fibroblast mRNA. Gene 15:215–223
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1553
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1553
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error