RNAs of definitive tombusviruses are about 4700 nucleotides in length; those of tentative members of the tombusvirus group, turnip crinkle virus (TCV) and glycine mottle virus (GMeV) are about 3900 nucleotides and those of saguaro cactus virus (SCV) and galinsoga mosaic virus (GMV) are about 3500 nucleotides. Hybridization with cDNA showed that there is some homology between the nucleic acids of different definitive tombusviruses. Analysis of the melting behaviour of heterologous cDNA:RNA hybrids suggests that different parts of the genome may be involved in hybrids between different combinations of tombusviruses. There is no homology between the nucleic acids of definitive tombusviruses and those of GMV, GMeV, SCV or TCV.
CoveyS. N.,
LomonossoffG. P.,
HullR.1981; Characterization of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts. Nucleic Acids Research 9:6735–6747
Di FrancoA.,
RussoM.,
MartelliG. P.1984; Ultrastructure and origin of cytoplasmic multivesicular bodies induced by carnation Italian ringspot virus. Journal of General Virology 65:1233–1237
GallitelliD.,
HullR.1985; Characterization of satellite RNAs associated with tomato bushy stunt virus and five other definitive tombusviruses. Journal of General Virology 66:1533–1543
HayesR. J.,
BuckK. W.,
BruntA. A.1984; Double-stranded and single-stranded subgenomic RNAs from plant tissue infected with tomato bushy stunt virus. Journal of General Virology 65:1239–1243
HollingsM.,
StoneM. O.1965; Studies of pelargonium leaf curl virus. II. Relationships to tomato bushy stunt and other viruses. Annals of Applied Biology 56:87–98
KafatosF. C.,
JonesC. W.,
EfstratiadisA.1979; Determination of nucleic acid sequence homologies and relative concentrations by a dot blot procedure. Nucleic Acids Research 6:1541–1552
KoenigR.,
AvgelisA.1983; Identification of a virus similar to the BS3 strain of tomato bushy stunt virus in eggplants. Phytopathologische Zeitschrift 106:349–353
LovisoloO.1957; Petunia: nuovo ospite naturale del virus del rachitismo cespuglioso del pomodoro. Bollettino della Stazione di patologia vegetale di Roma, Serie III 14:103–109
LovisoloO.,
AmbrosinoC.,
LiberatoriJ.,
PapaG.1964; Ricerche sul virus del rachitismo cespuglioso del pomodoro (tomato bushy stunt virus). I. Differenziazione per via elettrocinetica ed immunochimica del ceppo ‘Petunia’ dal ceppo BS-3. Atti dell’Accademia delle scienze 92:301
MartelliG. P.,
RussoM.,
QuacquarelliA.1977; Tombusviruses (tomato bushy stunt virus group). In The Atlas of Insects and Plant Viruses pp 257–279 Edited by
MaramoroschK.
New York: Academic Press;
MatthewsR. E. F.1982; Classification and nomenclature of viruses. Fourth Report of the International Committee on Taxonomy of Viruses. Intervirology 17:1–199
MauleA. J.,
HullR.,
DonsonJ.1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6:215–224
RussoM.,
Di FrancoA.,
MartelliG. P.1983; The fine structure of Cymbidium ringspot virus infections in host tissues. III. Role of peroxisomes in the genesis of multivesicular bodies. Journal of Ultrastructure Research 82:52–63
TaylorJ. M.,
IllmenseeR.,
SummersJ.1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
ThomasP. S.1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
WhiteB. A.,
BancroftF. C.1982; Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. Journal of Biological Chemistry 257:8569–8572