1887

Journal of General Virology header logo

Volume 66, Issue 7

Ia Antigens and Fc Receptors of Mouse Peritoneal Macrophages as Determinants of Susceptibility to Lactic Dehydrogenase Virus Free

Abstract

SUMMARY

The relationship between susceptibility of mouse peritoneal macrophages to lactic dehydrogenase-elevating virus (LDV) infection and expression of I region-coded antigens (Ia) on these cells was investigated. The proportion of Ia-positive cells in resident peritoneal macrophages from adult and suckling mice were 4 to 10% and 50 to 70% respectively. Approximately the same percentage of the cells were susceptible to LDV, as detected by fluorescent antibody staining. In adult mice, double-labelling experiments showed that most of the Ia-positive cells were LDV-infected. When the cells were cultured for more than 24 h , Ia-positive cells rapidly disappeared and the culture became resistant to LDV. Removal of Ia-positive cells by treatment with anti-Ia plus complement or enrichment using an anti-Ia-coated Petri dish simultaneously removed or enriched for LDV-susceptible cells. Treatment of cells with trypsin (1 mg/ml) removed their I-A and I-E antigens and simultaneously abolished susceptibility for LDV. When LDV was preincubated with subneutralizing amounts of antibody, infectivity for macrophages was enhanced and the proportion of LDV-infected cells was higher than that of Ia-positive cells. This suggests that Fc receptors on macrophages can act as receptors for LDV coated with antiviral IgG.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): enhancing antibody , Fc receptors , Ia antigens and LDV
© Journal of General Virology 1985
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1469
1985-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071469.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1469&mimeType=html&fmt=ahah

References

  1. Belardelli F., Vignaux F., Proietti E., Gresser I. 1984; Injection of mice with antibody to interferon renders peritoneal macrophages permissive for vesicular stomatitis virus and encephalomyocarditis virus. Proceedings of the National Academy of Sciences, U.S.A 81:602–606
     [Google Scholar]
  2. Beller D. I., Unanue E. R. 1981; Regulation of macrophage populations. II. Synthesis and expression of Ia antigens by peritoneal exudate macrophages is a transient event. Journal of Immunology 126:263–269
     [Google Scholar]
  3. Cafruny W. A., Plagemann P. G. W. 1982; Immune response to lactate dehydrogenase-elevating virus: serologically specific rabbit neutralising antibody to the virus. Infection and Immunity 37:1007–1012
     [Google Scholar]
  4. Dickler H. B., Sachs D. H. 1974; Evidence for identity or close association of the Fc receptor of B lymphocytes and alloantigens determined by the Ir region of the H-2 complex. Journal of Experimental Medicine 140:779–786
     [Google Scholar]
  5. De Buy H. G., Worthington M., Johnson M. L. 1971; Effect of an immunosuppressive agent cyclophosphamide on chronic lactic dehydrogenase virus viraemia of mice. Infection and Immunity 4:720–724
     [Google Scholar]
  6. Fukumoto T., McMaster W. R., Williams A. F. 1982; Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. European Journal of Immunology 12:237–243
     [Google Scholar]
  7. Halstead S. B., Chow J. S., Marchette N. J. 1973; Immunological enhancement of dengue virus replication. Nature New Biology 243:24–26
     [Google Scholar]
  8. Helenius A., Morein B., Fries E., Simons K., Robinson P., Schirrmacher V., Terhorst C., Strominger J. L. 1978; Human (HLA-A and HLA-B) and murine (H-2K and H-2D) histocompatibility antigens are cell surface receptors for Semliki Forest virus. Proceedings of the National Academy of Sciences, U.S.A 75:3846–3850
     [Google Scholar]
  9. Herman G., Du Buy H. G., Johnson M. L. 1966; Studies on the in vivo and in vitro multiplication of the LDH virus of mice. Journal of Experimental Medicine 123:985–998
     [Google Scholar]
  10. Inada T., Mims C. A. 1984; Mouse Ia antigens are receptors for lactate dehydrogenase virus. Nature, London 309:59–61
     [Google Scholar]
  11. Inada T., Mims C. A. 1985; Pattern of infection and selective loss of Ia positive cells in suckling and adult mice inoculated with lactic dehydrogenase virus. Archives of Virology (in press)
    [Google Scholar]
  12. Isakov N., Feldman M., Segal S. 1982; Acute infection of mice with lactic dehydrogenase virus (LDV) impairs the antigen-presenting capacity of their macrophages. Cellular Immunology 66:317–322
     [Google Scholar]
  13. Lu C. Y., Calamal E. G., Unanue E. R. 1979; A defect in the antigen-presenting macrophages from neonatal mice. Nature, London 282:327–329
     [Google Scholar]
  14. Madsen L. H., Rodkey L. A. 1976; A method for preparing IgG1 (Fab′)2 fragment using small amount of serum. Journal of Immunological Methods 9:355–361
     [Google Scholar]
  15. Mogensen S. C. 1979; Role of macrophages in natural resistance to virus infections. Microbiological Reviews 43:1–26
     [Google Scholar]
  16. Notkins A. L., Mahar S., Scheele C., Goffman J. 1966; Infectious virus-antibody complex in the blood of chronically infected mice. Journal of Experimental Medicine 124:81–97
     [Google Scholar]
  17. Oldstone M. B. A., Tishon A., Dutko F. J., Kennedy S. I. T., Holland J. J., Lampert P. W. 1980; Does the major histocompatibility complex serve as a specific receptor for Semliki Forest virus ?. Journal of Virology 34:256–265
     [Google Scholar]
  18. Peirls J. S. M., Porterfield J. S. 1979; Antibody-mediated enhancement of flavivirus replication in macrophage-like cell lines. Nature, London 282:509–511
     [Google Scholar]
  19. Peiris J. S. M., Gordon S., Unkeless J. C., Porterfield J. S. 1981; Monoclonal anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages. Nature, London 289:189–191
     [Google Scholar]
  20. Pierres M., Kourilsky F. M., Rebouah J. P., Dosseto M., Caillol D. 1980; Distinct epitopes on Ik gene products identified by monoclonal antibodies. European Journal of Immunology 10:950–957
     [Google Scholar]
  21. Porter D. D., Porter H. G., Deerhale B. B. 1968; Immunofluorescence assay for antigen and antibody in lactic dehydrogenase virus infection in mice. Journal of Immunology 102:431–436
     [Google Scholar]
  22. Rask L., Klareskog L., Ostberg L., Peterson P. A. 1975; Isolation and properties of a murine spleen cell Fc receptor. Nature, London 257:231–233
     [Google Scholar]
  23. Rowson K. E. K., Mahy B. W. J. 1975; Lactic dehydrogenase virus. Virology Monographs vol 13 New York: Springer-Verlag;
     [Google Scholar]
  24. Rowson K. E. K., Mahy B. W. J., Bendinelli M. 1966; Riley virus neutralizing activity in the plasma of infected mice with persistent viraemia. Virology 28:775–778
     [Google Scholar]
  25. Schirrmacher V., Halloran P., David C. S. 1975; Interaction of Fc receptors with antibodies against Ia antigens and other cell surface components. Journal of Experimental Medicine 141:1201–1209
     [Google Scholar]
  26. Snyder D. S., Beller D. L., Unanue E. R. 1982; Prostaglandins modulate macrophage Ia expression. Nature, London 299:163–165
     [Google Scholar]
  27. Stueckemann J. A., Ritzi D. M., Holth M., Smith M. S., Swart W. J., Cafruny W. A., Plagemann P. G. W. 1982a; Replication of lactic dehydrogenase-elevating virus in macrophages. 1. Evidence for cytocidal replication. Journal of General Virology 59:245–262
     [Google Scholar]
  28. Stueckemann J. A., Holth , Swart W. J., Kowalchyk K., Smith M. S., Wolstenholme A. J., Cafruny W. A., Plagemann P. G. W. 1982b; Replication of lactate dehydrogenase-elevating virus in macrophages. 2. Mechanism of persistent infection in mice and cell culture. Journal of General Virology 59:263–272
     [Google Scholar]
  29. Tong S. L., Stueckemann J., Plagemann P. G. W. 1977; Autoradiographic method for detection of lactate dehydrogenase-elevating virus-infected cells in primary mouse macrophage cultures. Journal of Virology 22:219–227
     [Google Scholar]
  30. Unanue E. R. 1981; The regulatory role of macrophages in antigenic stimulation. Part 2: Symbiotic relationship between lymphocytes and macrophages. Advances in Immunology 31:1–136
     [Google Scholar]
  31. Unkeless J. C. 1979; Characterisation of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. Journal of Experimental Medicine 150:580–596
     [Google Scholar]
  32. Wroblewski F., Landu J. S. 1955; Lactic dehydrogenase activity in blood. Proceedings of the Society for Experimental Biology and Medicine 90:210–213
     [Google Scholar]
  33. Wysocki L. J., Sato V. L. 1978; ‘Panning’ for lymphocytes: a method for cell selection. Proceedings of the National Academy of Sciences, U.S.A 75:2844–2848
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1469
Loading
Ia Antigens and Fc Receptors of Mouse Peritoneal Macrophages as Determinants of Susceptibility to Lactic Dehydrogenase Virus
J. Gen. Virol. 66, 1469 (1985); https://doi.org/10.1099/0022-1317-66-7-1469
/content/journal/jgv/10.1099/0022-1317-66-7-1469
/content/journal/jgv/10.1099/0022-1317-66-7-1469
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error