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Volume 66, Issue 7

Ia Antigens and Fc Receptors of Mouse Peritoneal Macrophages as Determinants of Susceptibility to Lactic Dehydrogenase Virus Free

Abstract

SUMMARY

The relationship between susceptibility of mouse peritoneal macrophages to lactic dehydrogenase-elevating virus (LDV) infection and expression of I region-coded antigens (Ia) on these cells was investigated. The proportion of Ia-positive cells in resident peritoneal macrophages from adult and suckling mice were 4 to 10% and 50 to 70% respectively. Approximately the same percentage of the cells were susceptible to LDV, as detected by fluorescent antibody staining. In adult mice, double-labelling experiments showed that most of the Ia-positive cells were LDV-infected. When the cells were cultured for more than 24 h , Ia-positive cells rapidly disappeared and the culture became resistant to LDV. Removal of Ia-positive cells by treatment with anti-Ia plus complement or enrichment using an anti-Ia-coated Petri dish simultaneously removed or enriched for LDV-susceptible cells. Treatment of cells with trypsin (1 mg/ml) removed their I-A and I-E antigens and simultaneously abolished susceptibility for LDV. When LDV was preincubated with subneutralizing amounts of antibody, infectivity for macrophages was enhanced and the proportion of LDV-infected cells was higher than that of Ia-positive cells. This suggests that Fc receptors on macrophages can act as receptors for LDV coated with antiviral IgG.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): enhancing antibody , Fc receptors , Ia antigens and LDV
© Journal of General Virology 1985
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1985-07-01
2023-03-30
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Ia Antigens and Fc Receptors of Mouse Peritoneal Macrophages as Determinants of Susceptibility to Lactic Dehydrogenase Virus
J. Gen. Virol. 66, 1469 (1985); https://doi.org/10.1099/0022-1317-66-7-1469
/content/journal/jgv/10.1099/0022-1317-66-7-1469
/content/journal/jgv/10.1099/0022-1317-66-7-1469
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