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Volume 66, Issue 6

Isolation and Characterization of Deletion Mutants of Herpes Simplex Virus Type 2 (Strain HG52) Free

Abstract

SUMMARY

We provide evidence that: (i) two variants lacking the I site at map coordinate 0.7 have been selected following I treatment of the DNA of herpes simplex virus type 2 strain HG52; (ii) one of these mutants had lost the 0.7 restriction site due to a deletion of approximately 150 base pairs and in the other the site loss was due to a similar sized sequence insertion; (iii) following I treatment, four variants with deletions ranging in size from 1.5 kb (in both TR and IR) to 9 kb in IR were isolated; (iv) substantial deletions in the long terminal repeat regions of HG52 are present with a frequency of 24% of genomes in the elite stock, a variant with a 3.75 kb deletion in IR making up 10% and one with a 1.5 kb deletion in both IR and TR making up 14%; (v) two of the variants isolated after I treatment of viral DNA were identical to the deletion prototype within the elite stock, suggesting that these variants were not generated as a result of I treatment but pre-existed in the viral DNA pool; (vi) the deletion variants were stably maintained during routine stock propagation, were viable and could be propagated as cloned populations; (vii) the deletions did not have a marked deleterious effect on the one-step growth kinetics of the virus.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deletion , HSV-2 and mutants
© Journal of General Virology 1985
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1985-06-01
2024-04-24
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Isolation and Characterization of Deletion Mutants of Herpes Simplex Virus Type 2 (Strain HG52)
J. Gen. Virol. 66, 1305 (1985); https://doi.org/10.1099/0022-1317-66-6-1305
/content/journal/jgv/10.1099/0022-1317-66-6-1305
/content/journal/jgv/10.1099/0022-1317-66-6-1305
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