1887

Abstract

SUMMARY

The first isolation of bluetongue virus (BTV) serotype 2 in the U.S.A. was in 1982 from a sentinel herd of cattle at Ona, Florida. Electrophoretic analysis of genome RNA revealed that all 16 serotype 2 isolates obtained from this focus of infection had one of two electropherotypes (designated Ona A and Ona B). All genome segments of Ona A and Ona B had detectable differences in electrophoretic mobility, with major differences noted for segments 1, 4, 5, 7, 8, 9 and 10. Electrophoretic comparison revealed that Ona A was indistinguishable from the African serotype 2 prototype isolated 23 years earlier. In 1983, Ona B, in the apparent absence of Ona A, was isolated from two additional cattle herds in Florida. These results suggest that Ona B may be a variant of Ona A that evolved as a result of mutation or reassortment with another BTV strain, and may be better adapted to the selective pressures found in southern Florida. Comparison of the electropherotypes of Ona B with two Florida isolates of serotype 13 and 17 and the prototypes of BTV 10, 11, 13 or 17 produced no evidence for reassortment between Ona A and any of these strains as the possible origin of Ona B.

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1985-06-01
2024-04-18
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References

  1. Barber T. L. 1979; Temporal appearance, geographic distribution and species of origin of bluetongue virus serotypes in the United States. American Journal of Veterinary Research 40:1654–1656
    [Google Scholar]
  2. Clarke L. N., Mccrae M. A. 1982; Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity 36:492–497
    [Google Scholar]
  3. Collisson E., Roy P. 1983; Analysis of the genomes of bluetongue virus serotype 10 vaccines and a recent BTV-10 isolate from Washington. American Journal of Veterinary Research 44:235–237
    [Google Scholar]
  4. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
    [Google Scholar]
  5. Foster N. M., Luedke A. J. 1968; Direct assay for bluetongue virus by intravascular inoculation of embryonating chicken eggs. American Journal of Veterinary Research 29:749–753
    [Google Scholar]
  6. Gambles R. M. 1949; Bluetongue of sheep in Cyprus. Journal of Comparative Pathology and Therapeutics 59:176–190
    [Google Scholar]
  7. Gibbs E. P. J., Greiner E. C., Alexander F. C. M., King T. H., Roach C. J. 1983a; Serological survey of ruminant livestock in some countries of the Caribbean region and South America for antibody to bluetongue vims. Veterinary Record 113:446–448
    [Google Scholar]
  8. Gibbs E. P. J., Greiner E. C., Taylor W. P., Barber T. L., House J. A., Pearson J. E. 1983b; Isolation of bluetongue virus serotype 2 from cattle in Florida: a serotype of bluetongue virus hitherto unrecognized in the Western Hemisphere. American Journal of Veterinary Research 44:2226–2228
    [Google Scholar]
  9. Gorman B. M. 1979; Variation in orbiviruses. Journal of General Virology 44:1–15
    [Google Scholar]
  10. Gorman B. M., Taylor J., Walker P. J., Davidson W. L., Brown F. 1981; Comparison of bluetongue type 20 with certain viruses of the bluetongue and Eubenangee serological groups of orbiviruses. Journal of General Virology 57:251–261
    [Google Scholar]
  11. Greiner E. C., Barber T. L., Pearson J. E., Kramer W. L., Gibbs E. P. J. 1985; Orbiviruses from Culicoides in Florida. In Proceedings of International Symposium on Bluetongue and Related Orbiviruses Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss; (in press)
    [Google Scholar]
  12. Groocock C. M., Campbell C. H. 1982; Isolation of an exotic serotype bluetongue virus from imported cattle in quarantine. Canadian Journal of Comparative Medicine 46:160–164
    [Google Scholar]
  13. Grubman M. J., Appleton J. A., Letchworth G. I. 1983; Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology 131:355–366
    [Google Scholar]
  14. Howell P. G. 1963; Bluetongue. In Emerging Diseases of Animals vol 66: pp 111–153 Food and Agriculture Organization of the United Nations, Department of Veterinary Services; Onderstepoort, South Africa:
    [Google Scholar]
  15. Hrdy D. B., Rosen L., Fields B. N. 1979; Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice. Journal of Virology 31:104–111
    [Google Scholar]
  16. Huismans H., Erasmus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  17. Jochim M. M., Jones S. C. 1976; Plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus in BHK-21 cells. American Journal of Veterinary Research 37:1345–1347
    [Google Scholar]
  18. Kahlon J., Sugiyama K., Roy P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  19. Knudson D. L., Butterfield W. K., Shope R. E., Walton T. E., Campbell C. H. 1982; Electrophoretic comparisons of the genomes of North American bluetongue viruses, one Australian virus, and three other related orbiviruses. Veterinary Microbiology 7:285–293
    [Google Scholar]
  20. Komarov A., Haig D. A. 1952; Identification of a strain of bluetongue virus isolated in Israel. Journal of the South African Veterinary Medical Association 23:153–156
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Rodger S. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  23. St. George T. D., Standfast H. A., Cybinski D. H. 1978; The isolation of a bluetongue virus from Culicoides collected in the Northern Territory of Australia. Australian Veterinary Journal 54:153–154
    [Google Scholar]
  24. Sangar D. V., Mertens P. P. C. 1983; Comparison of type 1 bluetongue virus protein synthesis in vivo and in vitro. In Double-Stranded RNA Viruses pp 183–191 Edited by Compans R. W., Bishop D. H. L. New York & London: Academic Press;
    [Google Scholar]
  25. Schnagl R. D., Rodger S. M., Holmes I. H. 1981; Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in Central Australia. Infection and Immunity 33:17–21
    [Google Scholar]
  26. Sellers R. F., Gibbs E. P. J., Herniman K. A. J., Pedgley D. E., Tucker R. M. 1979; Possible origin of the bluetongue epidemic in Cyprus, August 1977. Journal of Hygiene 83:547–555
    [Google Scholar]
  27. Squire K. R. E., Osburn B. I., Chuang R. Y., Doi R. H. 1983; A survey of electropherotype relationships of bluetongue virus isolates from the Western United States. Journal of General Virology 64:2103–2115
    [Google Scholar]
  28. Stott J. L., Barber T. L., Osburn B. I. 1978; Serotyping bluetongue virus: a comparison of plaque inhibition (disc) and plaque neutralization methods. 21st Annual Proceedings of the American Association of Veterinary Laboratory Diagnosticians pp 399–410
    [Google Scholar]
  29. Sugiyama K., Bishop D. H. L., Roy P. 1981; Analyses of the genomes of bluetongue viruses recovered in the United States. 1. Oligonucleotide fingerprint studies that indicate the existence of naturally occurring reassortant BTV isolates. Virology 114:210–217
    [Google Scholar]
  30. Sugiyama K., Bishop D. H. L., Roy P. 1982; Analyses of the genomes of bluetongue viruses recovered from different states of the United States and at different times. American Journal of Epidemiology 115:332–347
    [Google Scholar]
  31. Verwoerd D. W., Huismans H., Erasmus B. J. 1979; Orbiviruses. In Comprehensive Virology vol 14: pp 285–345 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  32. Walker P. J., Mansbridge J. N., Gorman B. M. 1980; Genetic analysis of orbiviruses by using RNase T1 oligonucleotide fingerprints. Journal of Virology 34:583–591
    [Google Scholar]
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