%0 Journal Article %A Roux, Laurent %A Beffy, Pascale %A Portner, Allen %T Three Variations in the Cell Surface Expression of the Haemagglutinin-Neuraminidase Glycoprotein of Sendai Virus %D 1985 %J Journal of General Virology, %V 66 %N 5 %P 987-1000 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-66-5-987 %K HN %K Sendai virus %K persistent infection %I Microbiology Society, %X SUMMARY The fate of the haemagglutinin-neuraminidase glycoprotein (HN) of Sendai virus in three types of infection was studied by measuring its sensitivity to endoglycosidase H and its rate of appearance and turnover at the cell surface. HN behaved differently in the three types of infection. When highly expressed at the surface, as in a lytic standard virus infection, HN accumulated at the surface in a stable form (half-life of disappearance from the surface ткв 10 h). When moderately expressed, as in a non-lytic standard virus plus defective interfering virus infection, HN reached the membrane normally, but turned over rapidly (half-life about 2 h) and was re-internalized. When poorly expressed, as in long-term persistent infection, HN did not reach the cell surface and appeared to be degraded before reaching it. In contrast to HN, the other viral glycoprotein, F0, exhibited a similar turnover rate at the cell surface in the three situations. However, when compared to surface expression in standard virus-infected cells under standardized conditions, F0 surface expression in persistently infected cells was reduced. This reduction correlates with a decreased maturation rate in these cells. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-5-987