@article{mbs:/content/journal/jgv/10.1099/0022-1317-66-5-1191, author = "Hibi, T. and Saito, Y.", title = "A Dot Immunobinding Assay for the Detection of Tobacco Mosaic Virus in Infected Tissues", journal= "Journal of General Virology", year = "1985", volume = "66", number = "5", pages = "1191-1194", doi = "https://doi.org/10.1099/0022-1317-66-5-1191", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-5-1191", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "TMV", keywords = "DIBA", keywords = "ELISA", keywords = "dot ELISA", abstract = "SUMMARY A dot immunobinding assay (DIBA) was modified for the rapid detection of plant viruses in infected tissues by avoiding non-specific reactions and minimizing the amounts of antibodies used. With this method, less than 1 ng of tobacco mosaic virus could be detected in several milligrams of infected tobacco leaves. This simple, rapid and sensitive assay should prove to be a useful and practical diagnostic technique for plant virus diseases.", }