1887

Abstract

SUMMARY

Sindbis virus maturation is inhibited by low NaCl medium in chick embryo cells and in one strain of BHK cells, but not in another strain of BHK cells which has a different passage history. The plasma membrane of the cells in which Sindbis virus maturation is resistant to low NaCl medium has a higher ratio of cholesterol to phospholipid than the other cells. Cholesterol-containing liposomes, but not cholesterol-free liposomes, can release Sindbis virus from low NaCl-inhibited cells. These results suggest that low NaCl medium may block Sindbis virus maturation by a mechanism which is influenced by the ratio of plasma membrane cholesterol to phospholipid.

Keyword(s): liposomes , maturation and Sindbis virus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-5-1171
1985-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/5/JV0660051171.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-5-1171&mimeType=html&fmt=ahah

References

  1. Bartlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  2. Bell J. R. Jr, Garry R. F., Waite M. R. F. 1978; Effect of low-NaCl medium on the envelope glycoproteins of Sindbis virus. Journal of Virology 25:764–769
    [Google Scholar]
  3. Bose H. R., Brundige M. 1972; Selective association of Sindbis virion proteins with different membrane fractions of infected cells. Journal of Virology 9:785–791
    [Google Scholar]
  4. Bosmann H. B., Hagopian A., Eylar E. H. 1968; Cellular membranes: the isolation and characterization of the plasma and smooth membranes of HeLa cells. Archives of Biochemistry and Biophysics 128:51–69
    [Google Scholar]
  5. Brown D. T. 1980; The assembly of alphaviruses. In The Togaviruses pp 473–501 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  6. David A. E. 1971; Lipid composition of Sindbis virus. Virology 46:711–720
    [Google Scholar]
  7. Folch J., Lees M., Sloane S. 1956; A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  8. Garry R. F., Bishop J. M., Parker S., Westbook K., Lewis G., Waite M. R. F. 1979; Sodium and potassium concentrations and the regulation of protein synthesis in Sindbis virus-infected chick cells. Virology 96:108–120
    [Google Scholar]
  9. Harrison S. C., David A., Jumblatt J., Darnell J. E. 1971; Lipid and protein organization in Sindbis virus. Journal of Molecular Biology 60:523–528
    [Google Scholar]
  10. Huang R. T. C., Wahn K., Klenk H. D., Rott R. 1980; Fusion between cell membranes and liposomes containing the glycoproteins of influenza virus. Virology 104:294–302
    [Google Scholar]
  11. Inbar M., Shintzky M. 1974; Increase of cholesterol level in the surface membrane of lymphoma cells and its inhibitory effect on ascites tumor development. Proceedings of the National Academy of Sciences, U. S. A 71:2128–2130
    [Google Scholar]
  12. Johnson D. C., Wittles M., Spear P. G. 1984; Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion. Journal of Virology 52:238–247
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. J., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Luukkonen A., Von Bonsdorff C. -H., Renkonen O. 1977; Characterization of Semliki Forest virus grown in mosquito cells. Comparison with the virus from hamster cells. Virology 78:331–335
    [Google Scholar]
  15. Mayne J. T., Rice C. M., Strauss E. G., Hunkapiller M. W., Strauss N. H. 1984; Biochemical studies of the maturation of the small Sindbis virus glycoprotein E3. Virology 134:338–357
    [Google Scholar]
  16. Mooney J. J., Dalrymple J. M., Alving G. R., Russell P. K. 1975; Interaction of Sindbis virus with liposomal model membranes. Journal of Virology 15:225–231
    [Google Scholar]
  17. Pessin J. E., Glaser M. 1980; Budding of Rous sarcoma virus and vesicular stomatitis virus from localized lipid regions in the plasma membrane of chicken embryo fibroblasts. Journal of Biological Chemistry 225:9044–9050
    [Google Scholar]
  18. Pfefferkorn E. R., Hunter H. S. 1963; Purification and partial chemical analysis of Sindbis virus. Virology 20:433–445
    [Google Scholar]
  19. Pierce J. S., Strauss E. G., Strauss J. H. 1974; Effect of ionic strength on the binding of Sindbis virus to chick cells. Journal of Virology 13:1030–1036
    [Google Scholar]
  20. Quigley J. P., Rifkin D. B., Reich E. 1972; Lipid studies of Rous sarcoma virus and host cell membranes. Virology 50:550–557
    [Google Scholar]
  21. Renkonen O., Kaarainen L., Simons K., Gahmber C. G. 1971; The lipid class composition of Semliki Forest virus and of plasma membranes of the host cells. Virology 46:318–326
    [Google Scholar]
  22. Rice C. M., Bell J. R., Hunkapiller M. W., Strauss E. G., Strauss J. H. 1982; Isolation and characterization of the hydrophobic COOH-terminal domains of the Sindbis virion glycoproteins. Journal of Molecular Biology 154:355–374
    [Google Scholar]
  23. Schefler W. C. 1969 Statistics for the Biological Sciences Reading Mass; Addison-Wesley:
    [Google Scholar]
  24. Schlesinger M. J., Schlesinger S., Burge B. W. 1972; Identification of a second glycoprotein in Sindbis virus. Virology 47:539–541
    [Google Scholar]
  25. Simons K., Garoff H., Helenius A. 1982; How an animal virus gets into and out of its host cell. Scientific American 218:58–70
    [Google Scholar]
  26. Straub S. X., Garry R. F., Magee W. E. 1974; Interferon induction by poly(I):poly(C) enclosed in phospholipid particles. Injection and Immunity 10:783–794
    [Google Scholar]
  27. Strauss E. G., Lenches E. M., Stamreich-Martin M. A. 1980; Growth and release of several alphaviruses in chick and BHK cells. Journal of General Virology 49:297–307
    [Google Scholar]
  28. Waite M. R. F., Pfefferkorn E. R. 1968; Effect of altered osmotic pressure on the growth of Sindbis virus. Journal of Virology 2:759–760
    [Google Scholar]
  29. Waite M. R. F., Pfefferkorn E. R. 1970; Inhibition of Sindbis virus production by media of low ionic strength: intracellular events and requirements for reversal. Journal of Virology 5:60–71
    [Google Scholar]
  30. Waite M. R. F., Brown D. T., Pfefferkorn E. R. 1972; Inhibition of Sindbis virus release by media of low ionic strength: an electron microscope study. Journal of Virology 10:537–544
    [Google Scholar]
  31. White J., Helenius A. 1980; pH-dependent fusion between the Semliki Forest virus membrane and liposomes. Proceedings of the National Academy of Sciences, U. S. A 77:3273–3277
    [Google Scholar]
  32. Zlatkis A., Zak B., Boyle A. J. 1953; A new method for the direct determination of serum cholesterol. Journal of Laboratory and Clinical Medicine 41:486–492
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-5-1171
Loading
/content/journal/jgv/10.1099/0022-1317-66-5-1171
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error