Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
AdamsD. S.,
MugenyaB. M.,
AllonbyE. W.,
BellJ. F.,
WaghelaS.,
HeinonenR.1983a; Observations On caprine arthritis-encephalitis in Kenya. Veterinary Record 112:227–228
AdamsD. S.,
Klevjer-AndersonP.,
CarlsonB. S.,
McguireT. C.,
GorhamJ. R.1983b; Transmission and control of caprine arthritis–encephalitis virus. American Journal of Veterinary Research 44:1670–1675
AdamsD. S.,
OliverR. E.,
AmeghinoE.,
DemartiniJ. C.,
VerwoerdD. W.,
HouwersD. J.,
WaghelaS.,
GorhamJ. R.,
HyllsethB.,
DawsonM.,
TrigoF. J.,
McguireT. C.1984; Serological evidence of caprine arthritis–encephalitis virus infection in eleven of fourteen countries tested. Veterinary Record 115:493–495
CrawfordT. B.,
AdamsD. S.1981; Caprine arthritis-encephalitis: clinical features and presence of antibody in selected goat populations. Journal of the American Veterinary Medical Association 178:713–719
CrawfordT. B.,
AdamsD. S.,
SandeR. D.,
GorhamJ. R.,
HensonJ. B.1980b; The connective tissue component of the caprine arthritis-encephalitis syndrome. American Journal of Pathology 100:443–454
Klevjer-AndersonP.,
CheeversW. P.1981; Characterization of the infection of caprine synovial membrane cells by the retrovirus caprine arthritis–encephalitis virus. Virology 110:113–119
RobersonS. M.,
McguireT. C.,
Klevjer-AndersonP.,
GorhamJ. R.,
CheeversW. P.1982; Caprine arthritis-encephalitis virus is distinct from visna and progressive pneumonia viruses as measured by genome sequence homology. Journal of Virology 44:755–758
ShapiroS. Z.,
AugustJ. T.1976; The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins. Journal of Immunological Methods 13:153–159