The steps from the precursor to the processed forms of a secreted glycoprotein (gA) of Marek′s disease virus (MDV) and of herpesvirus of turkeys (HVT) were examined by two-dimensional gel electrophoresis of immunoprecipitates from the cell lysate and medium of infected cultures with monoclonal antibodies. Differences between MDV-and HVT-gA were particularly observed in isoelectric points of the glycosylated or unglycosylated precursor forms.
ChirgwinJ. M.,
PrzybylaA. E.,
MacdonaldR. J.,
RutterW. J.1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
ChurchillA. E.,
ChubbR. C.,
BaxendaleW.1969; The attenuation, with loss of oncogenicity, of the herpes-type virus of Marek’s disease (strain HPRS-16) on passage in cell culture. Journal of General Virology 4:557–564
GahmbergG. G.,
JokinenM.,
KarhiK. K.,
AndersonL. C.1980; Effect of tunicamycin on the biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in the leukemia cell line K562. Journal of Biological Chemistry 255:2169–2175
GibbsC. P.,
NazerianK.,
VelicerL. F.,
KungH. J.1984; Extensive homology exists between Marek disease herpesvirus and its vaccine virus, herpesvirus of turkey. Proceedings of the National Academy of Sciences U.S.A 81:3365–3369
GlaubigerC.,
NazerianK.,
VelicerL. F.1983; Marek′s disease herpesvirus. IV. Molecular characterization of Marek’s disease herpesvirus A antigen. Journal of Virology 45:1228–1234
HiraiK.,
IkutaK.,
MaotaniK.,
KatoS.1984; Evaluation of DNA homology of Marek’s disease virus, herpesvirus of turkeys and Epstein-Barr virus under varied stringent hybridization conditions. Journal of Biochemistry 95:1215–1218
IkutaK.,
HonmaH.,
MaotaniK.,
UedaS.,
KatoS.,
HiraiK.1982; Monoclonal antibodies specific to and cross-reactive with Marek’s disease virus and herpesvirus of turkeys. Biken Journal 25:171–175
IkutaK.,
UedaS.,
KatoS.,
HiraiK.1983a; Most virus-specific polypeptides in cells productively infected with Marek′s disease virus or herpesvirus of turkeys possess cross-reactive determinants. Journal of General Virology 64:961–965
IkutaK.,
UedaS.,
KatoS.,
HiraiK.1983b; Monoclonal antibodies reactive with the surface and secreted glycoproteins of Marek’s disease virus and herpesvirus of turkeys. Journal of General Virology 64:2597–2610
IkutaK.,
UedaS.,
KatoS.,
HiraiK.1984a; Identification with monoclonal antibodies of glycoproteins of Marek’s disease virus and herpesvirus of turkeys related to virus neutralization. Journal of Virology 49:1014–1017
IkutaK.,
NakajimaK.,
UedaS.,
KatoA.,
HiraiK.1984b; Studies on the cross-reaction between Marek’s disease virus and herpesvirus of turkeys using monoclonal antibodies to major virus-specific polypeptides. Archives of Virology 81:337–343
Kaschka-DierichC.,
BornkammG. W.,
ThomssenR.1979; No homology detectable between Marek’s disease virus (MDV) DNA and herpesvirus of turkeys (HVT) DNA. Medical Microbiology and Immunology 165:223–239.
LeeL. F.,
LiuX.,
WitterR. L.1983; Monoclonal antibodies with specificity for three different serotypes of Marek′s disease virus in chickens. Journal of Immunology 130:1003–1006
LeeY. -S.,
TanakaA.,
SilverS.,
SmithM.,
NonoyamaM.1979; Minor DNA homology between herpesvirus of turkey and Marek’s disease virus?. Virology 93:277–280
LongP. A.,
Kaveh-YaminiP.,
VilicerL. F.1975; Marek’s disease herpesvirus. I. Production and preliminary characterization of Marek’s disease herpesvirus A antigen. Journal of Virology 15:1182–1191
OkazakiW.,
PurchaseH. G.,
BurmesterB. R.1970; Protection against Marek’s disease by vaccination with a herpesvirus of turkeys. Avian Disease 14:413–429
RossL. J. N.,
BiggsP. M.,
NewtonA. A.1973; Purification and properties of the ‘A’ antigen associated with Marek’s disease virus infections. Journal of General Virology 18:291–304
RothmanJ. E.,
KatzF. N.,
LodishH. F.1978; Glycosylation of a membrane protein is restricted to the growing polypeptide chain but is not necessary for insertion as a transmembrane protein. Cell 15:1447–1454
SchwarzR. T.,
RohrschneiderJ. M.,
SchmidtM. F. G.1976; Suppression of glycoprotein formation of Semliki Forest, influenza, and avian sarcoma virus by tunicamycin. Journal of Virology 19:782–791
Van ZaaneD.,
BrinkhofJ. M. A.,
GielkensA. L. J.1982; Molecular-biological characterization of Marek’s disease virus. II. Differentiation of various MDV and HVT strains. Virology 121:133–146