1887

Journal of General Virology

Volume 66, Issue 5

Injection of Anti-thy-1.2 Serum Breaks Genetic Resistance of Mice against Herpes Simplex Virus Free

Abstract

SUMMARY

Nude mice have been shown to be as resistant to intraperitoneal infection with herpes simplex virus type 1 (HSV) as their heterozygous littermates. Here we document that both activation of natural killer (NK) cells and interferon induction were normal in nu/nu mice after injection of HSV. Injection of silica caused increased mortality by HSV in C57BL/6 mice. Silica, in addition, led to a significant reduction of NK cell activity but had no effect on the interferon response. Treatment of C57BL/6 mice with anti-asialo GM1 (an antiserum with a predominant effect on NK cells) caused complete abolition of the NK cell response, but had no effect on interferon induction or virus-induced mortality. In further studies a monoclonal anti-thy-1.2 antibody was utilized which possessed high activity in depleting T cell responses in mice. Injection of anti-thy-1.2 decreased NK cell activation but was without effect on the interferon response. Unexpectedly, in view of the data in nu/nu mice, this antibody increased HSV-induced mortality in C57BL/6 mice. Similar data were obtained when anti-thy-1.2 was injected into nu/nu mice. Our results are compatible with the hypothesis that T cell precursors sensitive to anti-thy-1.2 present in homozygous nude mice play a role in resistance against HSV. Furthermore, the data in the euthymic mice may indicate a role of T cells in the primary resistance of mice against HSV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anti-thy-1.2 , HSV-1 , NK cells and T cells
© Journal of General Virology 1985
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1985-05-01
2021-10-23
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References

  1. Bancroft G. J., Shellam G. R., Chalmer J. E. 1981; Genetic influences on the augmentation of natural killer (NK) cells during murine cytomegalovirus infection: correlation with patterns of resistance. Journal of Immunology 126:988–994
     [Google Scholar]
  2. Burns W. H., Billups L. C., Notkins A. L. 1975; Thymus dependence of viral antigens. Nature, London 256:654–655
     [Google Scholar]
  3. Djeu J. Y., Heinbaugh J. A., Vieira W. D., Holden H. T., Herberman R. B. 1979; The effect of immuno-pharmacological agents on mouse natural cell-mediated cytotoxicity and on its augmentation by poly I:C. Immunopharmacology 1:231–244
     [Google Scholar]
  4. Engler H., Zawatzky R., Kirchner H., Armerding D. 1982; Experimental infection of inbred mice with herpes simplex virus. IV. Comparison of interferon production and natural killer cell activity in susceptible and resistant adult mice. Archives of Virology 74:239–247
     [Google Scholar]
  5. Habu S., Akamatsu K. -L., Tamaoki N., Okumura K. 1984; In vivo significance of NK cell on resistance against virus (HSV-1) infections in mice. Journal of Immunology 133:2743–2747
     [Google Scholar]
  6. Herberman R. B., Nunn M. E., Lavrin D. H. 1975; Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. I. Distribution of reactivity and specificity. International Journal of Cancer 16:216–229
     [Google Scholar]
  7. Hilfenhaus J., Christ H., Kohler R., Moser H., Kirchner H., Levy H. B. 1981; Efficacy of herpes simplex virus envelope antigen against herpes simplex virus type 1 and 2 infection in mice. Medical Microbiology and Immunology 169:225–235
     [Google Scholar]
  8. Hirsch M. S., Murphy F. A. 1968; Effects of anti-lymphoid sera on viral infections. Lancet 11:37–40
     [Google Scholar]
  9. Kapoor A. K., Buckmaster A., Nash A. A., Field H. J., Wildy P. 1982a; Role of neutralizing antibodies and T-cells in pathogenesis of herpes simplex virus infection in congenitally athymic mice. Immunology Letters 5:259–265
     [Google Scholar]
  10. Kapoor A. K., Nash A. A., Wildy P., Phelan J., Mclean S. C., Field H. J. 1982b; Pathogenesis of herpes simplex virus in congenitally athymic mice: the relative roles of cell-mediated and humoral immunity. Journal of General Virology 60:225–233
     [Google Scholar]
  11. Kasai M., Iwamori M., NAGAI Y., Okumura K., Tada T. 1980; A glycolipid on the surface of mouse natural killer cells. European Journal of Immmunology 10:175–180
     [Google Scholar]
  12. Kirchner H., Darai G., HIRT H. M., Keyssner K., Munk K. 1978a; In vitro mitogenic stimulation of murine spleen cells by herpes simplex virus. Journal of Immunology 120:641–645
     [Google Scholar]
  13. Kirchner H., Kochen M., Hirt H. M., Munk K. 1978b; Immunological studies of HSV infection of resistant and susceptible inbred strains of mice. Zeitschrift fur Immunitatsforschung 154:147–154
     [Google Scholar]
  14. Kirchner H., Fenkl H., Zawatzky R., Engler H., Becker H. 1980; Dissociation between interferon production induced by phytohemagglutinin and concanavalin A in spleen cell cultures of nude mice. European Journal of Immunology 10:224–225
     [Google Scholar]
  15. Kirchner H., Engler H., Schroder C. H., Zawatzky R., Storch E. 1983; Herpes simplex virus type 1-induced interferon production and activation of natural killer cells in mice. Journal of General Virology 64:437–441
     [Google Scholar]
  16. Lopez C. 1983; Natural resistance mechanism against herpes virus infections in health and disease. In Immunobiology of Herpes Simplex Virus Infection pp 45–69 Edited by Rouse B. T., Lopez C. Florida: CRC Press;
     [Google Scholar]
  17. Marcucci F., Klein B., Kirchner H., Zawatzky R. 1982; Production of high titres of interferon gamma by prestimulated murine spleen cells. European Journal of Immunology 12:787–790
     [Google Scholar]
  18. Metcalf J. F., Hamilton D. S., Reichert R. W. 1979; Herpetic keratitis in athymic (nude) mice. Infection and Immunity 26:1164–1171
     [Google Scholar]
  19. Mogensen S. C., Kerzel Andersen H. 1978; Role of activated macrophages in resistance of congenitally athymic nude mice to hepatitis induced by herpes simplex virus type 2. Infection and Immunity 19:792–798
     [Google Scholar]
  20. Mori R., Takeya K., Minashima Y., Tasaki T. 1965; Effect of thymectomy on experimental viral infections of Mice. I. Herpes simplex virus and coxsackie B5 virus. Proceedings of the Japan Academy 41:975–978
     [Google Scholar]
  21. Mori R., Tasaki T., Kimura G., Takeya K. 1967; Depression of acquired resistance against herpes simplex virus infection in neonatally thymectomized mice. Archil′ fur die gesamte Virusforschung 21:459–462
     [Google Scholar]
  22. Nagafuchi S., Oda H., Mori R., Taniguchi T. 1979; Mechanism of acquired resistance to herpes simplex virus infection as studied in nude mice. Journal of General Virology 44:715–723
     [Google Scholar]
  23. Nahmias A. L., Hirsch M. S., Kramer J. H., Murphy F. A. 1969; Effect of antithymocyte serum on herpesvirus hominis (type 1) infection in adult mice. Proceedings of the Society for Experimental Biology and Medicine 132:696–698
     [Google Scholar]
  24. Nash A. A., Leung K.-N., Wildy P. 1984; The T-cell mediated immune response of mice to herpes simplex virus. In The Herpesviruses vol. 4 Edited by Roizman B., Lopez C. New York: Plenum Press;
     [Google Scholar]
  25. Opitz H. G., Opitz U., Hewlett G., Schlumberger H. D. 1982; A new model for investigations of T-cell functions in mice: differential immunosuppressive effects of two monoclonal anti-thy-1.2 antibodies. Immunology 160:438–453
     [Google Scholar]
  26. Schindler L., Brucher J., Kirchner H. 1984; Protection of mice against infection with mouse hepatitis virus type 3 by injection of silica. Immunobiology 166:62–71
     [Google Scholar]
  27. Welsh R. M. 1981; Do natural killer cells play a role in virus infections?. Antiviral Research 1:5–12
     [Google Scholar]
  28. Zawatzky R., Hilfenhaus J., Kirchner H. 1979; Resistance of nude mice to herpes simplex virus and correlation with in vitro production of interferon. Cellular Immunology 47:424–428
     [Google Scholar]
  29. Zawatzky R., Hilfenhaus J., Marcucci F., Kirchner H. 1981; Experimental infection of inbred mice with herpes simplex virus type 1. I. Investigation of humoral and cellular immunity and of interferon induction. Journal of General Virology 53:31–38
     [Google Scholar]
  30. Zawatzky R., Gresser L., Demaeyer E., Kirchner H. 1982; Role of interferon in the resistance of C57BL/6 mice to different doses of herpes simplex virus type 1. Journal of Infectious Diseases 146:405–410
     [Google Scholar]
  31. Zisman B. R., Hirsch M. S., Allison A. C. 1970; Selective effects of anti-macrophage serum, silica, and antilymphocyte serum on pathogenesis of herpes simplex virus infection in young adult mice. Journal of Immunology 104:1155–1159
     [Google Scholar]
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Injection of Anti-thy-1.2 Serum Breaks Genetic Resistance of Mice against Herpes Simplex Virus
J. Gen. Virol. 66, 1087 (1985); https://doi.org/10.1099/0022-1317-66-5-1087
/content/journal/jgv/10.1099/0022-1317-66-5-1087
/content/journal/jgv/10.1099/0022-1317-66-5-1087
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