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Journal of General Virology header logo Journal of General Virology

Volume 66, Issue 5

Injection of Anti-thy-1.2 Serum Breaks Genetic Resistance of Mice against Herpes Simplex Virus No Access

Abstract

SUMMARY

Nude mice have been shown to be as resistant to intraperitoneal infection with herpes simplex virus type 1 (HSV) as their heterozygous littermates. Here we document that both activation of natural killer (NK) cells and interferon induction were normal in nu/nu mice after injection of HSV. Injection of silica caused increased mortality by HSV in C57BL/6 mice. Silica, in addition, led to a significant reduction of NK cell activity but had no effect on the interferon response. Treatment of C57BL/6 mice with anti-asialo GM1 (an antiserum with a predominant effect on NK cells) caused complete abolition of the NK cell response, but had no effect on interferon induction or virus-induced mortality. In further studies a monoclonal anti-thy-1.2 antibody was utilized which possessed high activity in depleting T cell responses in mice. Injection of anti-thy-1.2 decreased NK cell activation but was without effect on the interferon response. Unexpectedly, in view of the data in nu/nu mice, this antibody increased HSV-induced mortality in C57BL/6 mice. Similar data were obtained when anti-thy-1.2 was injected into nu/nu mice. Our results are compatible with the hypothesis that T cell precursors sensitive to anti-thy-1.2 present in homozygous nude mice play a role in resistance against HSV. Furthermore, the data in the euthymic mice may indicate a role of T cells in the primary resistance of mice against HSV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anti-thy-1.2 , HSV-1 , NK cells and T cells
© Journal of General Virology 1985
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1985-05-01
2025-07-10
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/content/journal/jgv/10.1099/0022-1317-66-5-1087
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Injection of Anti-thy-1.2 Serum Breaks Genetic Resistance of Mice against Herpes Simplex Virus
J. Gen. Virol. 66, 1087 (1985); https://doi.org/10.1099/0022-1317-66-5-1087
/content/journal/jgv/10.1099/0022-1317-66-5-1087
/content/journal/jgv/10.1099/0022-1317-66-5-1087
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