1887

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Volume 66, Issue 5

Purification of Herpes Simplex Virus Glycoproteins B and C Using Monoclonal Antibodies and Their Ability to Protect Mice against Lethal Challenge Free

Abstract

SUMMARY

Monoclonal antibodies which react with herpes simplex virus type 1 (HSV-1) glycoproteins, but do not neutralize infectivity, were prepared. The protein to which each monoclonal antibody was directed was determined by various techniques including their reaction with polypeptides from glycoprotein-deficient mutants after protein blotting, and also tests using passive haemagglutination. Monoclonal antibodies A7 and C3 were directed against a type-specific determinant on gC and a type-common determinant on gB respectively. In addition, A7 reacted only with the HFEM strain of HSV-1 and did not react with any of the 20 low-passage human isolates also tested. The monoclonal antibodies were used in immunoadsorption chromatography to purify individual glycoproteins from detergent extracts of HSV-1-infected cells. The ability of the monoclonal antibodies or purified glycoproteins to protect mice against a lethal encephalitis induced by intracerebral inoculation of HSV-1 was investigated. Passive immunization was not very effective; however, purified gC or a mixture of gB and its precursor pgB induced good levels of neutralizing antibody which persisted for at least 9 weeks and mice survived virus challenge.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): glycoproteins , HSV-1 , monoclonal antibodies and protection
© Journal of General Virology 1985
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1985-05-01
2024-04-25
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References

  1. Allen W. P., Rapp F. 1982; Concept review of genital herpes vaccines. Journal of Infectious Diseases 145:413–421
     [Google Scholar]
  2. Arvin A. M., Koropchak C. M., Yeager A. S., Pereira L. 1983; Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Infection and Immunity 40:184–189
     [Google Scholar]
  3. Balachandran N., Harnish D., Rawls W. E., Bacchetti S. 1982a; Glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies. Journal of Virology 44:344–355
     [Google Scholar]
  4. Balachandran N., Bacchetti S., Rawls W. E. 1982b; Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. Infection and Immunity 37:1132–1137
     [Google Scholar]
  5. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radio-iodinated protein A. Analytical Biochemistry 112:195–203
     [Google Scholar]
  6. Cappel R., Sprecher S., Rickaert F., Decuyper F. 1982; Immune response to a DNA free herpes simplex vaccine in man. Archives of Virology 73:61–67
     [Google Scholar]
  7. Cranage M. P., Mclean C. S., Buckmaster E. A., Minson A. C., Wildy P., Coombs R. R. A. 1983; The use of monoclonal antibodies in (reverse) passive haemagglutination tests for herpes simplex virus antigens and antibodies. Journal o] Medical Virology 11:295–306
     [Google Scholar]
  8. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: analysis by polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of Genera! Virology 5:499–509
     [Google Scholar]
  9. Dix R. D., Pereira L., Baringer J. R. 1981; Use of monoclonal antibody directed against herpes simplex virus glycoproteins to protect mice against acute virus-induced neurological disease. Infection and Immunity 34:192–199
     [Google Scholar]
  10. Eberle R., Courtney R. J. 1980; αA and αB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide. Journal of Virology 36:665–675
     [Google Scholar]
  11. Eberle R., Mou S.-W. 1983; Relative titers of antibodies to individual polypeptide antigens of herpes simplex virus type 1 in human sera. Journal of Infectious Diseases 148:436–444
     [Google Scholar]
  12. Eisenberg R. J., Ponce De Leon M., Pereira L., Long D., Cohen G. H. 1982; Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody. Journal of Virology 41:1099–1104
     [Google Scholar]
  13. Oilman S. C., Docherty J. J., Rawls W. E. 1981; Antibody responses in humans to individual proteins of herpes simplex viruses. Infection and Immunity 34:880–887
     [Google Scholar]
  14. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
     [Google Scholar]
  15. Herbert W. J. 1978; Passive haemagglutination with special reference to the tanned cell technique. In Handbook oj Experimental Immunology vol 1: pp 20.1–20.20 Edited by Weir D. M. Oxford: Blackwell Scientific Publications;
     [Google Scholar]
  16. Honess R. W., Watson D. H. 1974; Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. Journal of General Virology 22:171–185
     [Google Scholar]
  17. Howes E. L., Taylor W., Mitchison N. A., Simpson E. 1979; MHC matching shows that at least two T-cell subsets determine resistance to HSV. Nature, London 277:67–68
     [Google Scholar]
  18. Killington R. A., Randall R. E., Yeo J., Honess R. W., Halliburton I. W., Watson D. H. 1978; Observations on antigenic relatedness between viruses of the herpes simplex ‘neutroseron’. In Oncogenesis and Herpesviruses III pp 185–194 Edited by de-The G., Henle W., Rapp F. Lyon: International Agency for Research on Cancer;
     [Google Scholar]
  19. Killington R. A., Newhook L., Balachandran N., Rawls W. E., Bacchetti S. 1981; Production of hybrid cell lines secreting antibodies to herpes simplex virus type 2. Journal of Virological Methods 2:223–236
     [Google Scholar]
  20. Long D., Madara T. J., Ponce De Leon M., Cohen G. H., Montgomery P. C., Eisenberg R. J. 1984; Glycoprotein D protects mice against lethal challenge with herpes simplex virus types 1 and 2. Infection and Immunity 37:761–764
     [Google Scholar]
  21. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  22. Mckendall R. R. 1983; Delayed IgG-mediated clearance of herpes simplex virus type 1 from the CNS but not footpad during the early stages of infection: possible result of relative integrity of the blood-brain barrier. Journal of General Virology 64:1965–1972
     [Google Scholar]
  23. Manservigi R., Spear P. G., Buchan A. 1977; Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins. Proceedings of the National Academy of Sciences, U. S. A 74:3913–3917
     [Google Scholar]
  24. Nash A. A., Field H. J., Quartey-papafio R. 1980; Cell-mediated immunity in herpes simplex virus-infected mice: induction, characterization and antiviral effects of delayed type hypersensivity. Journal of General Virology 48:351–357
     [Google Scholar]
  25. Norrild B. 1980; Immunochemistry of herpes simplex virus glycoproteins. Current Topics in Microbiology and Immunology 90:67–106
     [Google Scholar]
  26. Palfreyman J. W., Haarr L., Cross A., Hope R. G., MarsdeN H. S. 1983; Processing of herpes simplex virus type 1 glycoproteins: two-dimensional gel analysis using monoclonal antibodies. Journal of General Virology 64:873–886
     [Google Scholar]
  27. Pereira L., Cassai E., Honess R. W., Roizman B., Terni M., Nahmias A. 1976; Variability in the structural polypeptides of herpes simplex 1 strains: potential application in molecular epidemiology. Infection and Immunity 13:211–220
     [Google Scholar]
  28. Pereira L., Dondero D., Norrild B., Roizman B. 1981; Differential immunologic reactivity and processing of glycoproteins αA and αB of herpes simplex virus types 1 and 2 made in Vero and Hep-2 cells. Proceedings of the National Academy of Sciences, U. S. A 78:5202–5206
     [Google Scholar]
  29. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. 1982; Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infection and Immunity 35:363–367
     [Google Scholar]
  30. Powell K. L., Watson D. H. 1975; Some structural antigens of herpes simplex virus type 1. Journal of General Virology 29:167–178
     [Google Scholar]
  31. Powell K. L., Buchan A., Sim C., Watson D. H. 1974; Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody. Nature, London 249:360–361
     [Google Scholar]
  32. Rager-zisman B., Allison A. C. 1976; Mechanism of immunologic resistance to herpes simplex virus 1 (HSV-1) infection. Journal of Immunology 116:35–40
     [Google Scholar]
  33. Raybould T. J. G., Chantler S. 1979; Comparison of activation and blocking procedures in the use of defined antigen substrate spheres (DASS) for quantitative and visual assessment of conjugate activity. Journal of Immunological Methods 27:309–318
     [Google Scholar]
  34. Raybould T. J. G., Chantler S. 1980; Serological differentiation between infected and vaccinated cattle by using purified soluble antigens from Brucella abortus in a hemagglutination system. Infection and Immunity 29:435–441
     [Google Scholar]
  35. Rector J. T., Lausch R. N., Oakes J. E. 1982; Use of monoclonal antibodies for analysis of antibody-dependent immunity to ocular herpes simplex virus type 1 infection. Infection and Immunity 38:168–174
     [Google Scholar]
  36. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
     [Google Scholar]
  37. Schrier R. D., Pizer L. I., Moorhead J. W. 1983; Type-specific delayed hypersensitivity and protective immunity induced by isolated herpes simplex virus glycoprotein. Journal of Immunology 130:1413–1418
     [Google Scholar]
  38. Sequeira P. J. L., Eldridge A. E. 1973; Treponemal haemagglutination test. British Journal of Venereal Diseases 49:242–248
     [Google Scholar]
  39. Sethi K. K. 1983; Effects of monoclonal antibodies directed against herpes simplex virus-specified glycoproteins on the generation of virus-specific and H-2-restricted cytotoxic T-lymphocytes. Journal of General Virology 64:2033–2037
     [Google Scholar]
  40. Sethi K. K., Omata Y., Schneweis K. E. 1983; Protection of mice from fatal herpes simplex virus type 1 infection by adoptive transfer to cloned virus-specific and H-2-restricted cytotoxic T lymphocytes. Journal of General Virology 64:443–447
     [Google Scholar]
  41. Show alter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infection and Immunity 34:684–692
     [Google Scholar]
  42. Sim C., Watson D. H. 1973; The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. Journal of General Virology 19:217–233
     [Google Scholar]
  43. Skinner G. R. B., Buchan A., Hartley C. E., Turner S. P., Williams D. R. 1980; The preparation, efficacy and safety of ‘antigenoid’ vaccine NFU, (S−L+) MRC towards prevention of herpes simplex virus infections in human subjects. Medical Microbiology and Immunology 169:39–51
     [Google Scholar]
  44. Snowden B. W., Kinchington P. R., Powell K. L., Halliburton I. W. 1985; Antigenic and biochemical analysis of gB of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammiliitis virus and equine herpesvirus type 1. Journal of General Virology 66:231–247
     [Google Scholar]
  45. Spear P. G. 1976; Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type-1 infected cells. Journal of Virology 17:991–1008
     [Google Scholar]
  46. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U. S. A 76:4350–4354
     [Google Scholar]
  47. Vantsis J. T., Wildy P. 1962; Interaction of herpes virus and HeLa cells: comparison of cell killing and infective center formation. Virology 17:225–232
     [Google Scholar]
  48. Voller A., Bidwell D. E., Bartlett A. 1979; The Enzyme Linked Immunosorbent Assay (ELISA). Guernsey; Dynatech Europe:
     [Google Scholar]
  49. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
     [Google Scholar]
  50. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
     [Google Scholar]
  51. Weis J. H., Enquist L. W., Salstrom J. S., Watson R. J. 1983; An immunologically active chimaeric protein containing herpes simplex virus type 1 glycoprotein D. Nature, London 302:72–74
     [Google Scholar]
  52. Wise T. G., Pa van P. R., Ennis F. A. 1977; Herpes simplex virus vaccines. Journal of Infectious Diseases 136:706–711
     [Google Scholar]
  53. Zweerink H. J., Corey L. 1982; Virus-specific antibodies in sera from patients with genital herpes simplex virus infection. Injection and Immunity 37:413–421
     [Google Scholar]
  54. Zweig M., Showalter S. D., Bladen S. V., Heilman C. J. Jr, Hampar B. 1983; Herpes simplex virus type 2 glycoprotein gF and type 1 glycoprotein αC have related antigenic determinants. Journal of Virology 47:185–192
     [Google Scholar]
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Purification of Herpes Simplex Virus Glycoproteins B and C Using Monoclonal Antibodies and Their Ability to Protect Mice against Lethal Challenge
J. Gen. Virol. 66, 1073 (1985); https://doi.org/10.1099/0022-1317-66-5-1073
/content/journal/jgv/10.1099/0022-1317-66-5-1073
/content/journal/jgv/10.1099/0022-1317-66-5-1073
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