1887

Abstract

SUMMARY

The proteins and RNAs of St. Abb’s Head virus have been analysed, and were found to be characteristic of the genus of the family Bunyaviridae. Two glycoproteins, G1 and G2 (mol. wt. 62K and 75K), and a nucleocapsid protein, N (mol. wt. 25K), were detected in infected cells by immunoprecipitation; the synthesis of N preceded the synthesis of G1 and G2. The glycoproteins were relatively cysteine-rich compared to the N protein, and the unglycosylated forms of G1 and G2 (using the inhibitor tunicamycin) had a mol. wt. of about 58K. Translation of mRNA from infected cells gave two immunoprecipitable products which are thought to be equivalent to N and G1/G2. Three RNA species were found in St. Abb’s Head virus nucleocapsids, and were estimated to be 8500 bases (L), 3600 bases (M) and 1900 bases (S) in length. At least one additional virus-specific RNA species was detected in infected cells. The similarity between the proteins and RNAs of St. Abb’s Head virus and Uukuniemi virus (the prototype of the genus) is discussed.

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1985-05-01
2021-10-19
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References

  1. Abraham G., Pattnaik A. K. 1983; Early RNA synthesis in Bunyamwera virus-infected cells. Journal of General Virology 64:1277–1290
    [Google Scholar]
  2. Barrett T., Mahy B. W. J. 1984; Molecular cloning of the nucleoprotein gene of canine distemper virus. Journal of General Virology 65:549–557
    [Google Scholar]
  3. Bishop D. H. L., Shope R. E. 1979; Bunyaviridae. In Comprehensive Virology vol 14: pp 1–36 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  4. Bishop D. H. L., Calisher C., Casals J., Chumakov M. P., Gaidomovich S. Y., Hannoun C., Lvov D. K., Marshall I. D., Oker-Blom N., Pettersson R. F., Porterfield J. S., Russell P. K., Shope R. E., Westaway E. G. 1980; Bunyaviridae. Intervirology 14:125–143
    [Google Scholar]
  5. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. II. Reconstruction of functional rough microsomes from heterologous components. Journal of Cellular Biology 67:852–862
    [Google Scholar]
  6. Bouloy M., Vialat P., Girard M., Pardigon N. 1984; A transcript from the S segment of the Germiston bunyavirus is uncapped and codes for the nucleoprotein and a nonstructural protein. Journal of Virology 49:717–723
    [Google Scholar]
  7. Collins P. L., Hightower L. E., Ball B. A. 1978; Transcription and translation of Newcastle disease virus mRNAs in vitro . Journal of Virology 28:324–336
    [Google Scholar]
  8. Elliott R. M. 1985; Identification of non-structural proteins encoded by viruses of the Bunyamwera serogroup (family Bunyaviridae). Virology (in press)
    [Google Scholar]
  9. Gahmberg N. 1984; Characterization of two recombination-complementation groups of Uukuniemi virus temperature-sensitive mutants. Journal oj General Virology 65:1079–1090
    [Google Scholar]
  10. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U. S. A 79:5818–5822
    [Google Scholar]
  11. Kascsak R. J., Lysons M. J. 1977; Bunyamwera virus. I. The molecular complexity of the virion RNA. Virology 82:37–47
    [Google Scholar]
  12. Kuismanen E. 1984; Posttranslational processing of Uukuniemi virus glycoproteins G1 and G2. Journal of Virology 51:806–812
    [Google Scholar]
  13. Kuismanen E., Hedman K., Saraste J., Pettersson R. F. 1982; Uukuniemi virus maturation: accumulation of virus particles and viral antigens in the Golgi complex. Molecular and Cellular Biology 2:1444–1458
    [Google Scholar]
  14. Kuismanen E., Bang B., Hurme M., Pettersson R. F. 1984; Uukuniemi virus maturation: immunofluorescence microscopy with monoclonal glycoprotein specific antibodies. Journal of Virology 51:137–146
    [Google Scholar]
  15. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–747
    [Google Scholar]
  16. McMaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U. S. A 74:4835–4838
    [Google Scholar]
  17. Molnar R., Eresikova M., Kubaszova T., Kubinyi D., Szabo J. B. 1973; Arboviruses in Hungary. Journal of Hygiene, Epidemiology, Microbiology and Immunology 17:1–10
    [Google Scholar]
  18. Nuttall P. A., Carey D., Reid H. W., Harrap K. A. 1981; Orbiviruses and bunyaviruses from a seabird colony in Scotland. Journal of General Virology 57:127–137
    [Google Scholar]
  19. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976; Structural proteins of La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  20. Oker-Blom N., Salminen A., Brummer-Korvenkontio M., Kääriäinen L., Weckström P. 1964; Isolation of some viruses other than tick-borne encephalitis viruses from Ixodes ricinus ticks in Finland. Annales medicinae experimentalis et biologiae Fenniae 42:109–112
    [Google Scholar]
  21. Parker M. D., Hewlett M. J. 1981; The 3′-terminal sequences of Uukuniemi and Inkoo virus RNA genome segments. In The Replication of Negative Strand Viruses pp 647–666 Edited by Bishop D. H. L., Compans R. W. New York & Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  22. Patterson J. L., Cabradilla C., Holloway B. P., Obijeski J. F., Kolakofsky D. 1983; Multiple leader RNAs and messenger RNAs are transcribed from the La Crosse virus small genome segment. Cell 33:791–799
    [Google Scholar]
  23. Pesonen M., Kuismanen E., Pettersson R. F. 1982; Monosaccharide sequence of protein-bound glycans of Uukuniemi virus. Journal of Virology 41:390–400
    [Google Scholar]
  24. Pettersson R., Kääriäinen L. 1973; The ribonucleic acids of Uukuniemi virus, a non-cubical tick-borne arbovirus. Virology 56:608–619
    [Google Scholar]
  25. Pettersson R., Kääriäinen L., Von Bonsdorff C. H., Oker-Blom N. 1971; Structural components of Uukuniemi virus, a non-cubical tick-borne arbovirus. Virology 46:721–729
    [Google Scholar]
  26. Pettersson R. F., Hewlett M. J., Baltimore D., Coffin J. M. 1977; The genome of Uukuniemi virus consists of three unique RNA segments. Cell 11:51–63
    [Google Scholar]
  27. Pringle C. R., Lees J. F., Clark W., Elliott R. M. 1984; Genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary DNA probes. Virology 135:244–256
    [Google Scholar]
  28. Pudney M., Varma M. G. R., Leake C. J. 1973; Establishment of a cell line from the South African clawed toad, Xenopus laevis . Experientia 29:466–467
    [Google Scholar]
  29. Robeson G., El said L. H., Brandt W., Dalrymple J., Bishop D. H. L. 1979; Biochemical studies on the phlebotomus fever group viruses (Bunyaviridae family). Journal of Virology 30:339–350
    [Google Scholar]
  30. Saikku P. 1973; Arbovirus in Finland. III. Uukuniemi virus antibodies in human, cattle and reindeer sera. American Journal of Tropica! Medicine and Hygiene 22:400–403
    [Google Scholar]
  31. Sekeyova M., Gresikova M., Stupalova S. 1970; Serological study in the distribution of Uukuniemi virus in man. Folia parasitologica 17:341–343
    [Google Scholar]
  32. Sleigh M. J., Both G. W., Brownlee G. G. 1979; A new method for the size estimation of the RNA genome segments of influenza virus. Nucleic Acids Research 6:1309–1321
    [Google Scholar]
  33. Traavik T., Mehl R. 1977; Uukuniemi group viruses isolated in Norway. Archives of Virology 54:317–331
    [Google Scholar]
  34. Ulmanen L., Seppala P., Pettersson R. F. 1981; In vitro translation of Uukuniemi virus-specific RNAs: identification of a non-structural protein and a precursor to the membrane glycoproteins. Journal of Virology 37:72–79
    [Google Scholar]
  35. Ushijima H., Klimas R., Kim S., Cash P., Bishop D. H. L. 1980; Characterization of the viral ribonucleic acids and structural polypeptides of Anopheles A, Bunyamwera, Group C, California, Capim, Guama, Patois and Simbu bunyaviruses. American Journal of Tropical Medicine and Hygiene 29:1441–1452
    [Google Scholar]
  36. Von Bonsdorff C. H., Pettersson R. 1975; Surface structure of Uukuniemi virus. Journal of Virology 16:1296–1307
    [Google Scholar]
  37. Watret G. E., Pringle C. R., Elliott R. M. 1985; Synthesis of bunyavirus-specific proteins in a continuous cell line (XTC-2) derived from Xenopus laevis . Journal of General Virology 66:473–482
    [Google Scholar]
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