%0 Journal Article %A Tóth, Miklós %A Endrész, Valéria %A Tóth, Sándor %A Béládi, Ilona %T Human Interferons Alpha and Beta Have More Potent Priming Activities than Interferon Gamma %D 1985 %J Journal of General Virology, %V 66 %N 4 %P 893-896 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-66-4-893 %K leukocytes %K priming %K interferon (HuIFN) %I Microbiology Society, %X SUMMARY The priming activities of human IFN-α, IFN-β and IFN-γ were compared on the same antiviral basis using human buffy coat leukocytes stimulated with Sendai virus or concanavalin A (Con A) to produce IFN-α or IFN-γ, respectively. Pretreatment of leukocytes with any type of IFN enhanced their IFN-α and IFN-γ production, but IFN-α and IFN-β had more potent priming activities than IFN-γ. IFN-α and IFN-γ did not potentiate the priming activity of each other in either the IFN-α- or the IFN-γ-producing system. Pretreatment of leukocytes with relatively high doses of IFN-α or IFN-β (1000 to 3000 IU/ml) resulted in a 40- to 50-fold increase in the IFN-γ production of Con A-stimulated leukocytes. This observation will be of use in producing IFN-γ with a high titre. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-4-893