1887

Abstract

Summary

The priming activities of human IFN-α, IFN-β and IFN-γ were compared on the same antiviral basis using human buffy coat leukocytes stimulated with Sendai virus or concanavalin A (Con A) to produce IFN-α or IFN-γ, respectively. Pretreatment of leukocytes with any type of IFN enhanced their IFN-α and IFN-γ production, but IFN-α and IFN-β had more potent priming activities than IFN-γ. IFN-α and IFN-γ did not potentiate the priming activity of each other in either the IFN-α- or the IFN-γ-producing system. Pretreatment of leukocytes with relatively high doses of IFN-α or IFN-β (1000 to 3000 IU/ml) resulted in a 40- to 50-fold increase in the IFN-γ production of Con A-stimulated leukocytes. This observation will be of use in producing IFN-γ with a high titre.

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/content/journal/jgv/10.1099/0022-1317-66-4-893
1985-04-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-4-893
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