1887

Abstract

SUMMARY

A comparison was made of the virulence and the infectivity for macrophages of (i) tissue culture-passed mouse cytomegalovirus (MCMV), (ii) salivary gland virus taken 3 weeks after infection (SGV 3w), and (iii) salivary gland virus taken 1 week after infection (SGV 1w). Salivary gland virus (3w) is known to be coated with non-neutralizing antibody, and is more virulent for newborn suckling mice and less infectious for macrophages than tissue culture-passed virus (TCV). Properties of SGV 1w were similar to those of TCV. Infectivity of SGV 3w for macrophages was significantly enhanced by treatment with trypsin (10 µg/ml) and at the same time virulence was lost. When SGV 1w or TCV were treated with trypsin the infectivity for macrophages was unaltered as long as the inoculum was adjusted to contain the same number of p.f.u. as assayed in MEF. Trypsin-treated SGV 3w was neutralized not by rabbit anti-mouse IgG Fc, but by anti-Fab, whereas untreated virus was neutralized by both of these anti-mouse immunoglobulins. These results are discussed in terms of the association of virulence with virion-bound antibody and Fc receptors on macrophages.

Keyword(s): macrophages , MCMV and virulence
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-4-879
1985-04-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/4/JV0660040879.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-4-879&mimeType=html&fmt=ahah

References

  1. Brautigam A. R., Dutko F. J., Olding L. B., Oldstone M. B. A. 1979; Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency. Journal of General Virology 44:349–359
    [Google Scholar]
  2. Chong K. T., Mims C. A. 1981; Murine cytomegalovirus particle types in relation to sources of virus and pathogenicity. Journal of General Virology 57:415–419
    [Google Scholar]
  3. Chong K. T., Gould J. J., Mims C. A. 1981; Neutralisation of different strains of murine cytomegalovirus (MCMV)-effect of in vitro passage. Archives of Virology 69:95–104
    [Google Scholar]
  4. Eizuru Y., Minamishima Y. 1979; Co-variation of pathogenicity and antigenicity in murine cytomegalovirus. Microbiology and Immunology 23:559–564
    [Google Scholar]
  5. Hudson J. B., Loh L., Mistra V., Judd B., Suzuki J. 1978; Multiple interactions between murine cytomegalovirus and lymphoid cells in vitro. Journal of General Virology 38:149–159
    [Google Scholar]
  6. Inada T., Chong K. T., Mims C. A. 1985; Enhancing antibodies, macrophages and virulence in mouse cytomegalovirus infection. Journal of General Virology 66:871–878
    [Google Scholar]
  7. Jordan M. C., Takagi J. L. 1983; Virulence characteristics of murine cytomegalovirus in cell and organ cultures. Infection and Immunity 41:841–843
    [Google Scholar]
  8. Mims C. A., Gould J. 1978; The role of macrophages in mice infected with murine cytomegalovirus. Journal of General Virology 41:143–153
    [Google Scholar]
  9. Mogensen S. C. 1979; Role of macrophages in natural resistance to virus infections. Microbiological Reviews 43:1–26
    [Google Scholar]
  10. Osborn J. E., Walker D. L. 1971; Virulence and attenuation of murine cytomegalovirus. Infection and Immunity 3:228–236
    [Google Scholar]
  11. Selgrade M. K., Osborn J. E. 1974; Roleof macrophages in resistance to murine cytomegalovirus. Infection and Immunity 10:1381–1390
    [Google Scholar]
  12. Selgrade M. K., Nedrud J. G., Collier A. M., Gardner D. E. 1981; Effect of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infection and Immunity 33:840–847
    [Google Scholar]
  13. Shanley J. D., Pesanti E. L. 1983; Murine peritoneal macrophages support murine cytomegalovirus replication. Infection and Immunity 41:1352–1359
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-4-879
Loading
/content/journal/jgv/10.1099/0022-1317-66-4-879
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error