RT Journal Article SR Electronic(1) A1 de Varennes, Amarilis A1 Davies, Jeffrey W. A1 Shaw, John G. A1 Maule, Andrew J.YR 1985 T1 A Reappraisal of the Effect of Actinomycin D and Cordycepin on the Multiplication of Cowpea Mosaic Virus in Cowpea Protoplasts JF Journal of General Virology, VO 66 IS 4 SP 817 OP 825 DO https://doi.org/10.1099/0022-1317-66-4-817 PB Microbiology Society, SN 1465-2099, AB SUMMARY Actinomycin D (AD) administered to cowpea mosaic virus-infected cowpea protoplasts immediately after inoculation inhibited virus multiplication, whereas late in the incubation period neither virus multiplication nor host DNA transcription were affected. The data obtained suggested that neither virus uncoating nor encapsidation were inhibited by AD. The inhibition of virus multiplication was manifested as a decrease in the level of progeny viral nucleoproteins and (+) and (-) viral RNAs. Cordycepin strongly inhibited coat protein production and (+) and (-) viral RNA synthesis throughout the incubation period., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-4-817