1887

Abstract

SUMMARY

Actinomycin D (AD) administered to cowpea mosaic virus-infected cowpea protoplasts immediately after inoculation inhibited virus multiplication, whereas late in the incubation period neither virus multiplication nor host DNA transcription were affected. The data obtained suggested that neither virus uncoating nor encapsidation were inhibited by AD. The inhibition of virus multiplication was manifested as a decrease in the level of progeny viral nucleoproteins and (+) and (-) viral RNAs. Cordycepin strongly inhibited coat protein production and (+) and (-) viral RNA synthesis throughout the incubation period.

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1985-04-01
2024-04-19
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References

  1. Alblas F., Bol J. F. 1977; Factors influencing the infection of cowpea mesophyll protoplasts by alfalfa mosaic virus. Journal of General Virology 36:175–185
    [Google Scholar]
  2. Baric R., Lineberger D. W., Johnson R. E. 1983; Reduced synthesis of Sindbis virus negative strand RNA in cultures treated with host transcription inhibitors. Journal of Virology 47:46–54
    [Google Scholar]
  3. Beach L. R., Ross J. 1978; Cordycepin. An inhibitor of newly synthesized globin messenger RNA. Journal of Biological Chemistry 253:2628–2632
    [Google Scholar]
  4. Cereghini S., Geoghegan T., Bergmann I., Brawerman G. 1979; Studies on the efficiency of translation and on the stability of actin messenger ribonucleic acid in mouse sarcoma ascites cells. Biochemistry 18:3153–3159
    [Google Scholar]
  5. Covey S. N., Turner D., Mulder G. 1983; A small DNA molecule containing covalently-linked ribonucleotides originates from the large intergenic region of the cauliflower mosaic virus genome. Nucleic Acids Research 11:251–264
    [Google Scholar]
  6. Dasgupta A. 1983; Purification of host factor required for in vitro transcription of poliovirus RNA. Virology 128:245–251
    [Google Scholar]
  7. Dasgupta A., Zabel P., Baltimore D. 1980; Dependence of the activity of poliovirus replicase on a host cell protein. Cell 19:423–429
    [Google Scholar]
  8. Dimitrieva T. M., Sheheglova M. V., Agol V. 1979; Inhibition of activity of encephalomyocarditis virus-induced RNA polymerase by antibodies against cellular components. Virology 92:271–277
    [Google Scholar]
  9. Dreyfuss G., Adam S. A., Choi Y. D. 1984; Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription. Molecular and Cellular Biology 4:415–423
    [Google Scholar]
  10. Franssen H., Leunissen J., Goldbach R., Lomonossoff G., Zimmern D. 1984; Homologous sequences in non-structural proteins from cowpea mosaic virus and picornaviruses. EMBO Journal 3:855–861
    [Google Scholar]
  11. Hibi T., Rezelman G., van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  12. Kessler-Icekson G., Singer R. H., Yaffe D. 1978; The capacity of polyadenylated RNA from myogenic cells treated with actinomycin D to direct protein synthesis in a cell-free system. European Journal of Biochemistry 88:403–410
    [Google Scholar]
  13. Kuznetov D. A., Husaev N. I., Khasigov P. Z., Nikolaev A. Ya. 1983; The effect of poly(A) segment size on the inhibition of hnRN A biosynthesis by cordycepin in rat brain cells. Biochimica et biophysica acta 740:220–222
    [Google Scholar]
  14. Maale G., Stein G., Mans R. 1975; Effects of cordycepin and cordycepin-triphosphate on polyadenylic and ribonucleic acid-synthesising enzymes from eukaryotes. Nature, London 255:80–82
    [Google Scholar]
  15. Maule A. J. 1983; Infection of protoplasts from several Brassica species with cauliflower mosaic virus following inoculation using polyethylene glycol. Journal of General Virology 64:2655–2660
    [Google Scholar]
  16. Maule A. J., Boulton M. I., Wood K. R. 1980; An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathologische Zeitschrijt 97:118–126
    [Google Scholar]
  17. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  18. Mayo M. A., Barker H. 1983; Effects of actinomycin D on the infection of tobacco protoplasts by four viruses. Journal of General Virology 64:1775–1780
    [Google Scholar]
  19. Morris-Krsinich B. A. M., Hull R., Russo M. 1979; Infection of turnip leaf protoplasts with turnip rosette virus. Journal of General Virology 43:339–347
    [Google Scholar]
  20. Motoyoshi F., Hull R. 1974; The infection of tobacco protoplasts with pea enation mosaic virus. Journal of General Virology 24:89–99
    [Google Scholar]
  21. Nair C. N., Panicali D. L. 1976; Polyadenylate sequences of human rhinovirus and poliovirus RNA and cordycepin sensitivity of virus replication. Journal of Virology 20:170–176
    [Google Scholar]
  22. Nassuth A., Alblas F., van Der Geest A. J. M., Bol J. F. 1983; Inhibition of alfalfa mosaic virus RNA and protein synthesis by actinomycin D and cycloheximide. Virology 126:517–524
    [Google Scholar]
  23. Otsuki Y., Takebe I., Honda Y., Kajita S., Matsui S. 1974; Infection of tobacco mesophyll protoplasts by potato virus X. Journal of General Virology 22:375–385
    [Google Scholar]
  24. Panicali D. L., Nair C. N. 1978; Effect of cordycepin triphosphate on in vitro RNA synthesis by picornavirus polymerase complexes. Journal of Virology 25:124–128
    [Google Scholar]
  25. Rottier P. J. M., Rezelman G., van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  26. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  27. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U,. S,. A 77:5201–5205
    [Google Scholar]
  28. van Der Meer J., Dorssers L., van Kammen A., Zabel P. 1984; The RNA-dependent RNA polymerase of cowpea is not involved in cowpea mosaic virus RNA replication: immunological evidence. Virology 132:413–425
    [Google Scholar]
  29. van Kammen A., De Jager C. P. 1978; Cowpea mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 197
    [Google Scholar]
  30. White J. L., Dawson W. O. 1979; Effect of cordycepin triphosphate on in vitro RNA synthesis by plant viral replicases. Journal of Virology 29:811–814
    [Google Scholar]
  31. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining the viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
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