The genetic and antigenic variation in 12 Sindbis (SIN) virus isolates from four zoogeographic regions (Paleoarctic, Ethiopian, Oriental and Australian) has been examined at a molecular level. RNase T1 oligonucleotide fingerprinting of genomic RNA from SIN isolates revealed that the primary structure of the RNA from viruses from each zoogeographic region was unique. The E1 and E2 glycoproteins and the capsid protein of two isolates from each zoogeographic region were compared by tryptic peptide mapping with the Egyptian prototype strain AR-339. Tryptic peptide maps of viruses from Sicily and the Ethiopian region were similar to those of the prototype; maps of isolates from the Oriental and Australia regions were different from each other and from those of the prototype strain. Viruses from each of the four zoogeographic regions were analysed antigenically by neutralization with polyclonal serum to AR-339 and by enzyme-linked immunosorbent assay with an anti-E2 monoclonal AR-339 antibody. Clear antigenic divergence of SIN isolates into two groups, representing the Paleoarctic-Ethiopian and Oriental-Australian regions were demonstrated. These results support a hypothesis which proposes that ancestral SIN virus diverged into two distinct groups. The genetic changes have resulted in further phenotypic divergence within the geographic varieties.
AaronsonR. P.,
YoungJ. F.,
PaleseP.1982; Oligonucleotide mapping: evaluation of its sensitivity by computer-simulation. Nucleic Acids Research 10:237–246
BergeT. O.1975International Catalogue of Arboviruses U.S. Department of Health. Education, and Welfare Publication (CDC) 75–8301 2nd edn Atlanta, Georgia: Centers for Disease Control;
ClewleyJ. P.,
GentschJ.,
BishopD. H. L.1977; Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. Journal of Virology 22:459–468
DalrympleJ. M.,
SchlesingerS.,
RussellP. K.1976; Antigenic characterization of two Sindbis virus envelope glycoproteins separated by isoelectric focusing. Virology 69:93–103
DohertyR. L.,
CarleyJ. G.,
MackerrasM. J.,
MarksE. N.1963; Studies on arthropod borne virus infections in Queensland. III. Isolations and characterization of virus strains from wild caught mosquitoes in North Queensland. Australian Journal of Experimental Biology and Medical Science 41:17–40
FranceJ. K.,
WyrickB. C.,
TrentD. W.1979; Biochemical and antigenic comparisons of the envelope glycoproteins of Venezuelan equine encephalomyelitis virus strains. Journal of General Virology 44:725–740
KarabatsosN.1975; Antigenic relationships of group A arboviruses by plaque reduction neutralization testing. American Journal of Tropical Medicine and Hygiene 24:527–532
KarabatsosN.,
BourneA. T. C.,
HendersonJ. R.1963; Antigenic variation among strains of western equine encephalomyelitis virus. Journal of Tropical Medicine and Hygiene 12:408–412
KinneyR. M.,
TrentD. W.1982; Conservation of tryptic peptides in the structural proteins of viruses in the Venezuelan equine encephalitis complex. Virology 121:345–362
MonathT. P.,
KinneyR. M.,
SchlesingerJ. J.,
BrandrissM. W.,
BresP.1983; Ontogeny of yellow fever 17D vaccine: RNA oligonucleotide fingerprint and monoclonal antibody analyses of vaccines produced world-wide. Journal of General Virology 64:627–637
PaleseP.,
BrandC.,
YoungJ. F.,
BaezM.,
SixR.,
KaselJ. A.1981; Molecular epidemiology of influenza viruses. Perspectives in Virology vol 6: pp 115–127 Edited by
PollardM.
New York: Alan R. Liss;
RiceC. M.,
StraussJ. H.1981; Nucleotide sequence of the26SmRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins. Proceedings of the National Academy of Sciences, U,. S,. A 78:2062–2066
RoehrigJ. T.,
DayJ. W.,
KinneyR. M.1982; Antigenic analysis of the surface glycoproteins of a Venezuelan equine encephalomyelitis virus (TC-83) using monoclonal antibodies. Virology 118:269–278
ShahK. V.,
JohnsonH. N.,
RaoT. R.,
RajogopalanP. K.,
LambaB. S.1960; Isolation of five strains of Sindbis virus in India. Indian Journal of Medical Research 48:300–308
SimmonsD. T.,
StraussJ. H.1972; Replication of Sindbis virus. I. Relative size and genetic content of 26S and 49S RNA. Journal of Molecular Biology 71:599–613
TaylorR. M.,
HurlbotH. S.,
WorkT. H.,
KingstonJ. R.,
FrothinghamT. E.1955; Sindbis virus: a newly recognized arthropod-transmitted virus. American Journal of Tropical Medicine and Hygiene 4:844–862
TrentD. W.,
GrantJ. A.1980; A comparison of New World alphaviruses in the Western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques. Journal of General Virology 47:261–282
VollerA.,
BidwellD.,
BartlettA.1976; Microplate enzyme immunoassays for the immunodiagnosis of virus infections. In Manual of Clinical Immunology pp 506–512 Edited by
RoseN. R.,
FriedmanH.
Washington, D. C.: American Society for Microbiology;
WeinbrenM. P.,
KokernotR. H.,
SmithburnK. C.1956; Strains of Sindbis like virus isolated from Culicine mosquitoes in the Union of South Africa. South African Medical Journal 30:631–636
YoungJ. F.,
DesselbergerU.,
PaleseP.1979; Evolution of human influenza A viruses in nature: sequential mutations in the genomes of new H1N1 isolates. Cell 18:73–83
YoungS. F.,
TaussigR.,
AaronsonR. P.,
PaleseP.1981; Advantages and limitations of the oligonucleotide mapping technique for the analysis of viral RNAs. In Replication of Negative Strand Viruses pp 209–215 Edited by
BishopD. H. L.,
CompansR. W.
Amsterdam: Elsevier/North-Holland;
YoungN.,
JohnsonK. M.1969; Antigenic variants of equine encephalitis virus: the geographic distribution and epidemiologic significance. American Journal of Tropical Medicine and Hygiene 89:286–307