1887

Abstract

SUMMARY

Two interferon-mediated enzymes, a 2-5A synthetase and a kinase that phosphorylates a 67000 mol. wt. (p67K) protein were found at variable levels in different organs of mice. Among the different strains of mice included in this study, germ-free mice had the lowest levels of these enzymes. The levels of 2-5A synthetase and p67K kinase were enhanced significantly in all mice following treatment with mouse ( + ) interferon. Here, we show that the presence of 2-5A synthetase and p67K kinase in different organs of normal mice (untreated) was due, at least in part, to a constant production of interferon under different physiological conditions. Accordingly, injection of normal mice with anti-mouse interferon ( + ) globulin led to a significant decrease in the level of 2-5A synthetase and p67K kinase. In conventional mice (C3H/He), the level of both of these enzymes was higher in female than in male animals and was decreased with age or when such animals were reared isolated in a pathogen-free protected unit. The levels of 2-5A synthetase and p67K kinase were also decreased in normal mice following injection with a powerful antibiotic against a very wide spectrum of Grampositive and Gram-negative bacteria. These results suggest that the production of interferon was induced continuously in normal mice. Such induction was mediated by both internal and external agents.

Keyword(s): enzymes , interferon induction and mice
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-4-711
1985-04-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/4/JV0660040711.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-4-711&mimeType=html&fmt=ahah

References

  1. Belardelli F., Vignaux F., Proietti E., Gresser I. 1984; Injection of mice with antibody to interferon renders peritoneal macrophages permissive for vesicular stomatitis virus and encephalomyocarditis virus. Proceedings of the National Academy of Sciences, U,. S,. A 81:602–606
    [Google Scholar]
  2. Bocci V. 1981; Production and role of interferon in physiological conditions. Biological Reviews 56:49–85
    [Google Scholar]
  3. Buffet-Janvresse C., Magard H., Robèrt N., Hovanessian A. G. 1983; Assay and the levels of 2–5A synthetase in lymphocytes of patients with viral, bacterial and autoimmune diseases. Annales d’Immunologic 134D:247–258
    [Google Scholar]
  4. Chabbert Y. A., Lutz A. 1981; Cefotaxime, syn isomer of a new methoxyimino cephalosporin with unusual activity. Nouvelle Presse Medicate 10:541–544
    [Google Scholar]
  5. Clover C. K., Zarkower A. 1980; Immunologic responses in colostrum-fed and colostrum-deprived calves. American Journal of Veterinary Research 41:1002–1007
    [Google Scholar]
  6. Drasar B. S., HILL H. R. (editors) 1974; The distribution of bacterial flora in the intestine. In Human Intestinal Flora pp 36–43 New York: Academic Press;
    [Google Scholar]
  7. Hovanessian A. G. 1979; Intracellular events in interferon-treated cells. Differentiation 15:139–151
    [Google Scholar]
  8. Hovanessian A. G., Rivière Y. 1980; Interferon-mediated induction of 2–5A synthetase and protein kinase in the liver and spleen of mice infected with Newcastle disease virus or injected with poly(I).poly(C). Annales de Virologie 131E:501–516
    [Google Scholar]
  9. Hovanessian A. G., Meurs E., Montagnier L. 1981a; Lack of systematic correlation between the interferon mediated antiviral state and the levels of 2-5A synthetase and protein kinase in three different types of murine cells. Journal of Interferon Research 1:179–180
    [Google Scholar]
  10. Hovanessian A. G., Rivière Y., Robèrt N., Svab J., Chamaret S., Guillon J. C., Montagnier L. 1981b; Protein kinase (pp67-IFN) in plasma and tissues of mice with high levels of circulating interferon. Annales de Virologie 132E:175–188
    [Google Scholar]
  11. Hovanessian A. G., Rivière Y., Krust B. 1983; Assay of interferon-mediated protein kinase activity from plasma and tissue extracts. Annals of Biochemistry 129:349–356
    [Google Scholar]
  12. Hudson J. A., Luckey T. D. 1964; Bacteria induced morphologic changes. Proceedings of the Society for Experimental Biology and Medicine 116:628–631
    [Google Scholar]
  13. Inoue T., Inoue K., Kitano K. 1980; Antibacterial antibodies in swine colostrum. American Journal of Veterinary Research 41:272–273
    [Google Scholar]
  14. Krishnan I., Baglioni C. 1980; 2′5′ oligo(A) polymerase activity in serum of mice infected with EMC virus or treated with interferon. Nature, London 285:485–488
    [Google Scholar]
  15. Krust B., Rivière Y., Hovanessian A. G. 1982; p67K kinase in different tissues and plasma of control and interferon-treated mice. Virology 120:240–246
    [Google Scholar]
  16. Rivière Y., Hovanessian A. G. 1983; Direct action of interferon and inducers of interferon on tumour cells in athymic nude mice. Cancer Research 43:4596–4599
    [Google Scholar]
  17. Saron M. F., Rivière Y., Hovanessian A. G., Guillon J. C. 1982; Chronic production of interferon in carrier mice congenitally infected with lymphocytic choriomeningitis virus. Virology 117:253–256
    [Google Scholar]
  18. Wood J. N., Hovanessian A. G. 1979; Interferon enhances 2-5A synthetase in embryonal carcinoma cells. Nature, London 282:74–76
    [Google Scholar]
  19. Youn J. K., Hovanessian A. G., Rivière Y., Gilbert H., Lacour F. 1983; Enhancement of natural killer cell activity and 2-5A synthetase in mice treated with polyadenylic–polyuridylic acid. Cellular Immunology 79:298–308
    [Google Scholar]
  20. Zawatzky R., Kirchner H., Demaeyer-Guignard J., Demaeyer E. 1982; An X-linked locus influences the amount of circulating interferon induced in the mouse by herpes simplex virus type 1. Journal of General Virology 63:325–332
    [Google Scholar]
  21. Zawatzky R., Dandoy F., Demaeyer-Guignard J., Demaeyer E. 1983; X-linked genes influencing early IFN levels in the mouse. In The Biology of the Interferon System 1983 pp 77–82 Edited by DeMaeyer E., Schellekens H. Amsterdam, New York & Oxford: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-4-711
Loading
/content/journal/jgv/10.1099/0022-1317-66-4-711
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error