1887

Abstract

SUMMARY

The coding part of a murine interferon alpha (MuIFN-) gene was cloned into an expression plasmid containing the simian virus 40 early promoter and the rabbit -globin polyadenylation signal. This construct was transfected into Chinese hamster ovary cells, together with a plasmid containing the Ecogpt gene as a selection marker. Resulting colonies were assayed for constitutive interferon production and analysed for integration of MuIFN- genes. There was no obvious correlation between the number of genes integrated and the amount of interferon produced. The highest producer, designated CHO-pSV10EF-3, contained four copies of the mouse gene and constitutively secreted up to 100000 International Units of interferon per ml per day. The MuIFN- subspecies produced by this clone was characterized by analysis of its antiviral activity on heterologous cells, heparin-Sepharose affinity chromatography and chromatofocusing. The results obtained indicate that it is identical or closely related to a minor component present in conventional MuIFN- preparations.

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/content/journal/jgv/10.1099/0022-1317-66-4-685
1985-04-01
2023-02-08
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