1887

Abstract

SUMMARY

Larvae of the pine emperor moth, , naturally infected with β virus (NβV), contained a second serologically unrelated virus which we have called ω virus (NωV). NωV had a buoyant density of 1.285 g/ml in CsCl and yielded a single major polypeptide of 65000 daltons on gel electrophoresis. The particles of NωV were morphologically distinguishable from those of viruses described by others, and tryptic peptide analyses indicated that NωV protein was distinct from that of NβV and NɛV. Both NωV and NβV contained a small stable fraction of particles with buoyant densities of about 1.33 g/ml.

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1985-03-01
2021-10-17
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References

  1. Daubert S. D., Bruening G., Najarian R. C. 1978; Protein bound to the genome RNAs of cowpea mosaic virus. European Journal of Biochemistry 92:45–51
    [Google Scholar]
  2. Dingman C. W., Peacock A. C. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide gels. Biochemistry 7:668–674
    [Google Scholar]
  3. Elder J. H., Pickett R. A., Hampton J., Lerner R. A. 1977; Radioiodination of proteins in single polyacrylamide gel slices. Journal of Biological Chemistry 252:6510–6515
    [Google Scholar]
  4. Finch J. T., Crowther R. A., Hendry D. A., Struthers J. K. 1974; The structure of Nudaurelia capensis β virus: the first example of a capsid with icosahedral surface symmetry T = 4. Journal of General Virology 24:191–200
    [Google Scholar]
  5. Greenwood L. K., Moore N. F. 1981; The Nudaurelia fi group of small RNA viruses of insects: serological comparison of four members. Microbiologica 4:271–280
    [Google Scholar]
  6. Hendry D. A., Bekker M. F., Van Regenmortel M. H. V. 1968; A non-inclusion virus of the pine emperor moth, Nudaurelia cytherea capensis Stoll. South African Medical Journal 42:117
    [Google Scholar]
  7. Hendry D. A., Moore N. F., Morris T. J., Reinganum C., Scorn P. 1984; An overview of the Nudaurelia p virus group. Abstracts of the Sixth International Congress of Virology, Sendai, Japan p 341
    [Google Scholar]
  8. Juckes I. R. M. 1970; Viruses of the pine emperor moth. Bulletin of the South African Society of Plant Pathology and Microbiology 4:18
    [Google Scholar]
  9. Juckes I. R. M. 1974 Purification and characterisation of the viruses of the pine emperor moth Nudaurelia cytherea capensis (Fabr.) Ph. D. thesis University of Cape Town;
    [Google Scholar]
  10. Juckes I. R. M. 1979; Comparison of some biophysical properties of the Nudaurelia β and ε viruses. Journal of General Virology 42:89–94
    [Google Scholar]
  11. Juckes I. R. M., Longworth J. F., Reinganum C. 1973; A serological comparison of some nonoccluded insect viruses. Journal of Invertebrate Pathology 21:119–120
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Mapoles J. E., Anderegg J. W., Rueckert R. R. 1978; Properties of poliovirus propagated in medium containing cesium chloride: implications for picornaviral structure. Virology 90:103–111
    [Google Scholar]
  14. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Fourth Report of the International Committee on Taxonomy of Viruses. Intervirology 17:1–199
    [Google Scholar]
  15. Moore N. F., Tinsley T. W. 1982; The small RNA-viruses of insects. Brief Review. Archives of Virology 72:229–245
    [Google Scholar]
  16. Moore N. F., Greenwood L. K., Rixon K. R. 1981; Studies on the capsid protein of several members of the Nudaurelia fl group of small RNA viruses of insects. Microbiologica 4:59–71
    [Google Scholar]
  17. Morris T. J., Hess R. T., Pinnock D. E. 1979; Physicochemical characterization of a small RNA virus associated with baculovirus infection in Trichoplusia ni. Intervirology 11:238–247
    [Google Scholar]
  18. Polson A., Stannard L., Tripconey D. 1970; The use of haemocyanin to determine the molecular weight of Nudaurelia cytherea capensis fl virus by direct particle counting. Virology 41:680–687
    [Google Scholar]
  19. Reinganum C., Robertson J. S., Tinsley T. W. 1978; A new group of RNA viruses from insects. Journal of General Virology 40:195–202
    [Google Scholar]
  20. Rowlands D. J., Shirley M. W., Sangar D. V., Brown F. 1975; A high density component in several vertebrate enteroviruses. Journal of General Virology 29:223–234
    [Google Scholar]
  21. Struthers J. K., Hendry D. A. 1974; Studies of the protein and nucleic acid components of Nudaurelia capensis β virus. Journal of General Virology 22:355–362
    [Google Scholar]
  22. Tripconey D. 1970; Studies on a non–occluded virus of the pine tree emperor moth. Journal of Invertebrate Pathology 15:268–275
    [Google Scholar]
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