%0 Journal Article %A Chesters, P. M. %A McCance, D. J. %T Human Papillomavirus Type 16 Recombinant DNA Is Maintained as an Autonomously Replicating Episome in Monkey Kidney Cells %D 1985 %J Journal of General Virology, %V 66 %N 3 %P 615-620 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-66-3-615 %K eukaryotic transfection %K HPV %K episomal replication %K expression vector %I Microbiology Society, %X SUMMARY Human papillomavirus type 16 (HPV16) DNA cloned in the expression vector pSV2-neo has been shown to be maintained in transfected monkey kidney cells as an autonomously replicating episome at a level of 2 to 10 copies per cell. Integration of pSV2-neo/HPV16 DNA with the host genome occurred in transfected mouse fibroblasts. Neither type of cell appeared to be phenotypically transformed. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-3-615