1887

Abstract

SUMMARY

A coding sequence at the 5′ end of mRNA 4 of the coronavirus MHV-JHM was determined by M13/chain-terminator sequencing of cloned cDNA. An open reading frame of 417 bases with the potential to encode a polypeptide of mol. wt. 15200 (139 residues) was identified. The 3′ end of the open reading frame overlapped by 16 bases the start of an open reading frame found in mRNA 5. The translation product of mRNA 4 was predicted to be a basic polypeptide rich in threonine. It had a large hydrophobic region near the amino terminus and a basic carboxy terminus. An intracellular, virus-specific polypeptide, which has been previously described as having a mol. wt. of 14000 to 14500 has the size and charge characteristics of such a translation product.

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/content/journal/jgv/10.1099/0022-1317-66-3-593
1985-03-01
2021-10-16
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References

  1. Armstrong J., Smeekens S., Spaan W., Rottier P., van Der Zeijst B. 1984a; Cloning and sequencing the nucleocapsid and El genes of coronavirus. Advances in Experimental Medicine and Biology 173:155–162
    [Google Scholar]
  2. Armstrong J. A., Niemann H., Smeekens S., Rottier P., Warren G. 1984b; Sequence and topology of a model intracellular membrane protein, El glycoprotein, from a coronavirus. Nature, London 308:751–752
    [Google Scholar]
  3. Brayton P. R., Stohlman S. A., Lai M. M. C. 1984; Further characterization of mouse hepatitis virus RNA-dependent RNA polymerases. Virology 133:197–201
    [Google Scholar]
  4. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  5. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  6. Lai M. M. C., Patton C. D., Baric R. S., Stohlman S. A. 1983; Presence of leader sequences in the mRNA of mouse hepatitis virus. Journal of Virology 46:1027–1033
    [Google Scholar]
  7. Leibowitz I. L., Weiss S. R., Paavlsola E., Bond C. W. 1982; Cell free translation of murine coronavirus RNA. Journal of Virology 43:905–913
    [Google Scholar]
  8. Rottier P. J. M., Spaan W. J. M., Horzinek M. C., van Der Zeijst B. A. M. 1981; Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes. Journal of Virology 38:20–26
    [Google Scholar]
  9. Siddell S. 1983; Coronavirus JHM: coding assignments of subgenomic mRN As. Journal of General Virology 64:113–125
    [Google Scholar]
  10. Siddell S., Wege H., Barthel A., Ter Meulen V. 1981; Intracellular protein synthesis and the in vitro translation of coronavirus JHM mRNA. Advances in Experimental Medicine and Biology 142:193–207
    [Google Scholar]
  11. Siddell S., Wege H., Ter Meulen V. 1983; The biology of coronaviruses. Journal of General Virology 64:761–776
    [Google Scholar]
  12. Skinner M. A., Siddell S. 1983; Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein. Nucleic Acids Research 15:5045–5054
    [Google Scholar]
  13. Skinner M. A., Ebner D., Siddell S. G. 1985; Coronavirus MHV-JHM mRNA 5 has a sequence arrangement which potentially allows translation of a second, downstream open reading frame. Journal of General Virology 66:581–592
    [Google Scholar]
  14. Spaan W., Delius H., Skinner M., Armstrong J., Rottier P., Smeekens S., van Der Zeijst B. A. M., Siddell S. G. 1983; Coronavirus mRNA synthesis involves fusion of non-contiguous sequences. EMBO Journal 2:1839–1844
    [Google Scholar]
  15. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:473–4751
    [Google Scholar]
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