1887

Abstract

SUMMARY

cDNAs prepared from viral genomic RNA purified from two strains of infectious bronchitis virus (IBV) (Beaudette and M41) have been cloned into pBR322. Three of these clones, which contain the complete sequences of mRNA A for both strains, except for the leader sequences which are only present on the subgenomic messenger RNAs, have been sequenced using the dideoxy method. The sequences are similar for both strains, each containing a single long open reading frame of 1227 bases which predicts a polypeptide of molecular weight approximately 45000. The genome position and size of this predicted polypeptide are consistent with it being the gene for the nucleocapsid protein. The amino acid sequence shows considerable homology with those of the nucleocapsids of murine hepatitis virus strains A59 and JHM. The major difference between the sequences determined for the two IBV strains is that the 3′ non-coding region of the Beaudette strain contains a 184 base segment which is not present in the M41 strain.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-3-573
1985-03-01
2023-02-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/3/JV0660030573.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-3-573&mimeType=html&fmt=ahah

References

  1. Anderson S. 1981; Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Research 9:3015–3027
    [Google Scholar]
  2. Armstrong J., Smeekens S., Rottier P. 1983; Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. Nucleic Acids Research 11:883–891
    [Google Scholar]
  3. Bankier A., Barrell B. G. 1983; Shotgun DNA sequencing. In Techniques in the Life Sciences (Biochemistry), vol. B 5: Techniques in Nucleic Acid Biochemistry pp. B508 1–34 Edited by Flavell R. A. Ireland: Elsevier;
    [Google Scholar]
  4. Beaudette F. R., Hudson C. B. 1937; Cultivation of the virus of infectious bronchitis virus. Journal of the American Veterinary Medical Association 90:51–60
    [Google Scholar]
  5. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
    [Google Scholar]
  6. Boursnell M. E. G., Brown T. D. K. 1984; Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B. Gene 29:87–92
    [Google Scholar]
  7. Brown T. D. K., Boursnell M. E. G. 1984; Avian infectious bronchitis virus genomic RNA contains sequence homologies at the intergenic boundaries. Virus Research 1:15–24
    [Google Scholar]
  8. Brown T. D. K., Boursnell M. E. G., Binns M. M. 1984; A leader sequence is present on mRNA A of avian infectious bronchitis virus. Journal of General Virology 65:1437–1442
    [Google Scholar]
  9. Cavanagh D. 1981; Structural polypeptides of coronavirus IBV. Journal of General Virology 53:93–103
    [Google Scholar]
  10. Collins M. S., Alexander D.J. 1980; Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates. Archives of Virology 63:239–251
    [Google Scholar]
  11. Geilhausen H. E., Ligon F. B., Lukert P. D. 1972; The pathogenesis of virulent and avirulent avian infectious bronchitis virus. Archiv für die gesamte Virusforschung 40:285–290
    [Google Scholar]
  12. Grunstein M., Hogness D. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A 72:3961–3965
    [Google Scholar]
  13. Gubler U., Hoffman B. I. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  14. Hong G. F. 1981; A method for sequencing single-stranded cloned DNA in both directions. Bioscience Reports 1:243–252
    [Google Scholar]
  15. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  16. Leibowitz J. L., Wilhelmsen K. C., Bond C. W. 1981; The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM. Virology 114:39–51
    [Google Scholar]
  17. Lenard J., Compans R. W. 1974; The membrane structure of lipid-containing viruses. Biochimica et biophysics acta 344:51–94
    [Google Scholar]
  18. Macnaughton M. R., Madge M. G., Davies H. A., Dourmashkin R. R. 1977; Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. Journal of Virology 24:821–825
    [Google Scholar]
  19. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  20. Nagy E., Lomniczi B. 1979; Polypeptide patterns of infectious bronchitis virus serotypes fall into two categories. Archives of Virology 61:341–345
    [Google Scholar]
  21. Oshiro L. S. 1973; Coronaviruses. In Ultrastructure of Animal Viruses and Bacteriophages: An Atlas pp 331–343 Edited by Dalton A. J., Haguenau F. New York: Academic Press;
    [Google Scholar]
  22. Rottier P., Spaan W. J. M., Horzinek M. C., van Der Zeijst B. A. M. 1981; Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes. Journal of Virology 38:20–26
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  24. Siddell S. G. 1983; Coronavirus JHM: coding assignments of subgenomic mRNAs. Journal of General Virology 64:113–125
    [Google Scholar]
  25. Siddell S., Wege H., Barthel A., Ter Meulen V. 1980; Coronavirus JHM: cell-free synthesis of structural protein p60. Journal of Virology 33:10–17
    [Google Scholar]
  26. Siddell S. G., Barthel A., Termeulen V. 1981; Coronavirus JHM: a virion-associated protein kinase. Journal of General Virology 52:235–243
    [Google Scholar]
  27. Siddell S., Wege H., Ter Meulen V. 1983; The biology of coronaviruses. Journal of General Virology 64:761–776
    [Google Scholar]
  28. Skinner M. A., Siddell S. G. 1983; Coronavirus JHM: nucleotide sequence of the mRNA that encodes the nucleocapsid protein. Nucleic Acids Research 11:5045–5054
    [Google Scholar]
  29. Staden R. 1982a; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  30. Staden R. 1982b; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  31. Staden R. 1984a; A computer program to enter DNA gel reading data into a computer. Nucleic Acids Research 12:499–503
    [Google Scholar]
  32. Staden R. 1984b; Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Research 12:521–538
    [Google Scholar]
  33. Stern D. F. 1983; Organization of the IBV genome. EMBO Workshop. ‘Molecular Biology and Pathogenesis of Coronaviruses’ Zeist, The Netherlands:
    [Google Scholar]
  34. Stern D. F., Kennedy S. I. T. 1980a; Coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA. Journal of Virology 34:665–674
    [Google Scholar]
  35. Stern D. F., Kennedy S. I. T. 1980b; Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. Journal of Virology 36:440–449
    [Google Scholar]
  36. Stern D. F., Sefton B. M. 1984; Coronavirus multiplication: the locations of genes for the virion proteins on the avian infectious bronchitis virus genome. Journal of Virology 50:22–29
    [Google Scholar]
  37. Stern D. F., Burgess L., Sefton B. M. 1982; Structural analysis of virion proteins of the avian coronavirus infectious bronchitis virus. Journal of Virology 42:208–219
    [Google Scholar]
  38. Stohlman S. A., Lai M. M. C. 1979; Phosphoproteins of murine hepatitis viruses. Journal of Virology 32:672–675
    [Google Scholar]
  39. Stohlman S., Fleming J. O., Patton C. D., Lai M. M. C. 1983; Synthesis and subcellular location of the murine coronavirus nucleocapsid protein. Virology 130:527–532
    [Google Scholar]
  40. Sturman L. S. 1977; Characterization of a coronavirus. Virology 77:637–649
    [Google Scholar]
  41. Sturman L. S., Holmes K. V., Behnke J. 1980; Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. Journal of Virology 33:449–462
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-3-573
Loading
/content/journal/jgv/10.1099/0022-1317-66-3-573
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error