1887

Abstract

SUMMARY

Fifteen proteins were detected in Vero cells infected by dengue type 2 (DEN-2) virus that were not observed in mock-infected cells, namely P98, p82, P67, GP60, gp54, GP46, p30, p28, gp22, GP20, p18, gp16, p15, p14 and gp13. With the exceptions of gp54 and gp13, polypeptides corresponding to those listed above were also observed in DEN-2 virus-infected C6/36 cells. Pulse-chase labelling experiments suggested a possible precursor-product relationship between p30 and p28, and between gp22 and GP20. Peptide mapping and immunoprecipitation experiments showed that the major glycoproteins GP60, GP46 and GP20 were unrelated. Immunoprecipitations of infected cells with antiserum prepared against the DEN-2 soluble complement-fixing (SCF) antigen demonstrated that this antigen is equivalent to the non-structural glycoprotein GP46. The envelope glycoprotein (E) from virus grown in C6/36 cells migrated faster through polyacrylamide gels containing SDS than E from virus grown in Vero cells. [H]Mannose-labelled glycopeptides of GP60, GP46 and GP20 were separated by gel filtration and by electrophoresis in Tris-borate gels; in addition, the polypeptides synthesized in infected cells in the presence of tunicamycin were analysed. The results revealed heterogeneity among the glycan units of GP60 and GP46.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-3-559
1985-03-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/3/JV0660030559.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-3-559&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Boulton R. W., Westaway E. G. 1972; Comparison of togaviruses: Sindbis virus (group A) and Kunjin virus (group B). Virology 49:283–289
    [Google Scholar]
  3. Cardiff R. D., McCloud T. G., Brandt W. E., Russell P. K. 1970; Molecular size and charge relationships of the soluble complement-fixing antigens of dengue viruses. Virology 41:569–572
    [Google Scholar]
  4. Cardiff R. D., Brandt W. E., McCloud T. O., Shapiro D., Russell P. K. 1971; Immunological and biophysical separation of dengue-2 antigens. Journal of Virology 7:15–23
    [Google Scholar]
  5. Cleaves G. R., Dubin D. T. 1979; Methylation status of intracellular dengue type 2 40 S RNA. Virology 96:159–165
    [Google Scholar]
  6. Cleveland D. W., Fischer S. G., Kirschner M. W., La U. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  7. Hayes E. C., Wright L. L., Zweerink H. J. 1978; Staphylococcal protein A-Sepharose columns and the characterization of measles virus-specific polypeptides in persistently infected cells. Analytical Biochemistry 91:276–282
    [Google Scholar]
  8. Igarashi A. 1978; Isolation of a Singh’s Aedes albopiclus cell clone sensitive to dengue and Chikungunya viruses. Journal of General Virology 40:531–544
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Iuukkonen A., Von Bonsdorff C.-H., Renkonen O. 1977; Characterization of Semliki Forest virus grown in mosquito cells. Comparison with the virus from hamster cells. Virology 78:331–335
    [Google Scholar]
  11. McCloud T. G., Cardiff R. D., Brandt W. E., Chiewsilp D., Ussell P. K. 1971; Separation of dengue strains on the basis of a nonstructural antigen. American Journal of Tropical Medicine and Hygiene 20:964–968
    [Google Scholar]
  12. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  13. Poliquin L., Shore G. C. 1980; A method for efficient and selective recovery of membrane glycoproteins from concanavalin A-Sepharose using media containing sodium dodecyl sulfate and urea. Analytical Biochemistry 109:460–465
    [Google Scholar]
  14. Porterfield J. S. 1980; Antigenic characteristics and classification of Togaviridae. In The Togaviruses pp 1346 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  15. Roehrig J. T., Gorski D., Schlesinger M. J. 1982; Properties of monoclonal antibodies directed against the glycoproteins of Sindbis virus. Journal of General Virology 59:421–425
    [Google Scholar]
  16. Russell P. K., Brandt W. E., Dalrymple J. M. 1980; Chemical and antigenic structure of flaviviruses. In The Togaviruses pp 503–529 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  17. Schlesinger M. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  18. Schlesinger R. W. 1977; Viral replication in cell cultures. In Dengue Viruses, Virology Monographs vol 16: pp 49–72 Edited by Gard S., Hallauer C. Vienna: Springer-Verlag;
    [Google Scholar]
  19. Schlesinger R. W. 1981; General review of dengue and dengue hemorrhagic fever. In Dengue Hemorrhagic Fever 1981 Proceedings of the First ICMR Seminar, Kobe, Japan, November 21–22. Edited by Hotta S. Kobe: International Center for Medical Research, Kobe University School of Medicine;
    [Google Scholar]
  20. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. 1971; The proteins of Japanese encephalitis virus. Virology 44:108–124
    [Google Scholar]
  21. Smith T. J., Brandt W. E., Swanson J. L., McCown J. M., Buescher E. L. 1970; Physical and biological properties of dengue-2 virus and associated antigens. Journal of Virology 5:524–532
    [Google Scholar]
  22. Stohlman S. A., Wisseman V. L., Eylar O. R., Silverman D. J. 1975; Dengue virus-induced modifications of host cell membranes. Journal of Virology 16:1017–1026
    [Google Scholar]
  23. Stohlman S. A., Eylar O. R., Wisseman C. L. 1976; Isolation of the dengue virus envelope glycoprotein from membranes of infected cells by concanavalin An affinity chromatography. Journal of Virology 18:132–140
    [Google Scholar]
  24. Stollar V. 1969; Studies on the nature of dengue viruses. IV. The structural proteins of type 2 dengue virus. Virology 39:426–438
    [Google Scholar]
  25. Stollar V., Stollar B. D., Koo R., Harrap K. A., Schlesinger R. W. 1976; Sialic acid contents of Sindbis virus from vertebrate and mosquito cells. Virology 69:104–115
    [Google Scholar]
  26. Svitkin Y. V., Ugarova T. Y., Chernovskaya T. V., Lyapustin V. N., Laskevich V. A., Agol V. I. 1981; Translation of tick-borne encephalitis virus (flavivirus) genome in vitro, synthesis of two structural polypeptides. Virology 110:26–34
    [Google Scholar]
  27. Trent D. W., Qureshi A. A. 1971; Structural and nonstructural proteins of Saint Louis encephalitis virus. Journal of Virology 7:379–388
    [Google Scholar]
  28. Wechsler S. L., Rustigian R., Stallcup K. C., Byers K. B., Winston S. H., Fields B. N. 1979; Measles virus-specified polypeptide synthesis in two persistently infected HeLa cell lines. Journal of Virology 31:677–684
    [Google Scholar]
  29. Weitzman S., Scott V., Keegstra K. 1979; Analysis of glycopeptides as borate complexes by polyacrylamide gel electrophoresis. Analytical Biochemistry 97:438–449
    [Google Scholar]
  30. Wengler G., Wengler G., Gross H. J. 1978; Studies on virus-specific nucleic acids synthesized in vertebrate and mosquito cells infected with flaviviruses. Virology 89:423–437
    [Google Scholar]
  31. Westaway E. G. 1966; Assessment and application of a cell line from pig kidney for plaque assay and neutralization tests with twelve group B arboviruses. American Journal of Epidemiology 84:439–456
    [Google Scholar]
  32. Westaway E. G. 1973; Proteins specified by group B togaviruses in mammalian cells during productive infections. Virology 51:454–465
    [Google Scholar]
  33. Westaway E. G. 1975; The proteins of Murray Valley encephalitis virus. Journal of General Virology 27:283–292
    [Google Scholar]
  34. Westaway E. G. 1977; Strategy of the flavivirus genome: evidence for multiple internal initiation of translation of proteins specified by Kunjin virus in mammalian cells. Virology 80:320–335
    [Google Scholar]
  35. Westaway E. G. 1980; Replication of flaviviruses. In The Togaviruses pp 531–581 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  36. Westaway E. G., Reedman B. M. 1969; Proteins of the group B arbovirus Kunjin. Journal of Virology 4:688–693
    [Google Scholar]
  37. Westaway E. G., Shew M. 1977; Proteins and glycoproteins specified by the flavivirus Kunjin. Virology 80:309–319
    [Google Scholar]
  38. Westaway E. G., McKimm J. L., McLeod L. G. 1977; Heterogeneity among flavivirus proteins separated in slab gels. Archives of Virology 53:305–312
    [Google Scholar]
  39. Westaway E. G., Schlesinger R. W., Dalrymple J. M., Trent D. W. 1980; Nomenclature of flavivirus-specified proteins. Intervirology 14:114–117
    [Google Scholar]
  40. Westaway E. G., Speight G., Endo L. 1984; Gene order of translation of the flavivirus Kunjin: further evidence of internal initiation in vivo. Virus Research 1:333–350
    [Google Scholar]
  41. Wright P. J., Warr H. M. 1985; Peptide mapping of envelope-related glycoproteins specified by the flaviviruses Kunjin and West Nile. Journal of General Virology 66:597–601
    [Google Scholar]
  42. Wright P. J., Warr H. M., Westaway E. G. 1980; Preliminary characterization of glycopeptides derived from glycoproteins specified by the flavivirus Kunjin. Virology 104:482–486
    [Google Scholar]
  43. Wright P. J., Warr H. M., Westaway E. G. 1981; Synthesis of glycoproteins in cells infected by the flavivirus Kunjin. Virology 109:418–427
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-3-559
Loading
/content/journal/jgv/10.1099/0022-1317-66-3-559
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error