@article{mbs:/content/journal/jgv/10.1099/0022-1317-66-3-529, author = "Crawford, Allan M. and Sheehan, Cathy", title = "Replication of Oryctes Baculovirus in Cell Culture: Viral Morphogenesis, Infectivity and Protein Synthesis", journal= "Journal of General Virology", year = "1985", volume = "66", number = "3", pages = "529-539", doi = "https://doi.org/10.1099/0022-1317-66-3-529", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-3-529", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "morphogenesis", keywords = "baculovirus", keywords = "protein synthesis", keywords = "Oryctes rhinoceros", abstract = "SUMMARY The sequence of events in the replication of Oryctes baculovirus in DSIR-HA-1179 cells began with the uptake of virus particles by pinocytosis at the plasma membrane. Seven h after infection, enveloped virus particles were observed inside a cleared area in the nucleus. Virus was released from cells by budding through the plasma membrane where a second unit membrane was acquired. The infectivity of virus particles with a single envelope purified from within cells was not significantly different from that of virus which had acquired the second envelope by budding. Twenty-seven structural virus proteins were identified by gradient polyacrylamide gel electrophoresis. The synthesis of eight of the major structural proteins was detected by pulse labelling infected cells with l-[35S]methionine. No late protein synthesis, as observed in subgroup A baculoviruses, was detected in this subgroup C baculovirus.", }