1887

Abstract

SUMMARY

The sequence of events in the replication of baculovirus in DSIR-HA-1179 cells began with the uptake of virus particles by pinocytosis at the plasma membrane. Seven h after infection, enveloped virus particles were observed inside a cleared area in the nucleus. Virus was released from cells by budding through the plasma membrane where a second unit membrane was acquired. The infectivity of virus particles with a single envelope purified from within cells was not significantly different from that of virus which had acquired the second envelope by budding. Twenty-seven structural virus proteins were identified by gradient polyacrylamide gel electrophoresis. The synthesis of eight of the major structural proteins was detected by pulse labelling infected cells with -[S]methionine. No late protein synthesis, as observed in subgroup A baculoviruses, was detected in this subgroup C baculovirus.

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1985-03-01
2022-01-28
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