1887

Abstract

SUMMARY

Whereas human cytomegalovirus (HCMV) did not replicate in human embryonal carcinoma (EC) cells, it did replicate in some of the differentiated cells arising following the exposure of TERA-2-derived human EC cells to retinoic acid. On the other hand, retinoic acid did not induce a permissive state in several other diverse human cell lines, including an EC line, 2102Ep, which did not differentiate in response to this agent. Also, both TERA-2 and 2102Ep EC cells differentiated to a limited extent when grown at low cell density and a few of these cells became permissive for HCMV. Thus, susceptibility is the result of differentiation and not due to a direct effect of retinoic acid on viral replication. The nature of the block to HCMV replication in human EC cells is unknown, but viral DNA could be detected in the nucleus within an hour of infection and there was an increased anchorage-independent growth of undifferentiated and differentiated cells following HCMV infection. Viral replication is not subject to a general block in these cells, since another herpesvirus, herpes simplex virus type 1, replicated well.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-3-509
1985-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/3/JV0660030509.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-3-509&mimeType=html&fmt=ahah

References

  1. Andrews P. W. 1982; Human embryonal carcinoma cells in culture do not synthesize fibronectin until they differentiate. International Journal of Cancer 30:567–571
    [Google Scholar]
  2. Andrews P. W. 1984; Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Developmental Biology 103:285–293
    [Google Scholar]
  3. Andrews P. W., Bronson D. L., Benham F., Strickland S., Knowles B. B. 1980; A comparative study of eight human testicular tumor-derived cell lines. International Journal of Cancer 26:269–280
    [Google Scholar]
  4. Andrews P. W., Goodfellow P. N., Shevinsky L., Bronson D. L., Knowles B. B. 1982; Cell surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenetic differentiation in culture. International Journal of Cancer 29:523–531
    [Google Scholar]
  5. Andrews P. W., Damjanov I., Simon D., Banting G. S., Carlin C., Dracopoli N. C., Fogh J. 1984; Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2; differentiation in vivo and in vitro. Laboratory Investigation 50:147–162
    [Google Scholar]
  6. Cheng C., Praszkier J. 1982; Regulation of type 5 adenovirus replication in murine teratocarcinoma cell lines. Virology 123:45–59
    [Google Scholar]
  7. Colimon R., Mazeron M. C., Roseto A., Perol Y. 1984; Monoclonal antibodies (McAb): a new approach to the diagnosis of CMW infections. In CMV: Pathogenesis and Prevention of Human Injection. Birth Dejects: Original Article Series vol 20 No. 1. Edited by Plotkin S. A., Michelson S., Pagano J., Rapp F. New York: Alan R. Liss;
    [Google Scholar]
  8. Cotte C., Raghavan D., McIlhinney R. A. J., Monaghan P. 1982; Characterisation of a new human cell line derived from xenografted embryonal carcinoma. In Vitro 18:730–749
    [Google Scholar]
  9. Damjanov I., Andrews P. W. 1983; Ultrastructural differentiation of a clonal human embryonal carcinoma cell line in vitro. Cancer Research 43:2190–2198
    [Google Scholar]
  10. Dutko F. J., Oldstone M. B. A. 1981; Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. Journal of Experimental Medicine 154:1636–1651
    [Google Scholar]
  11. Furukawa T., Fioretti A., Plotkin S. A. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
    [Google Scholar]
  12. Gautsch J. W., Wilson M. C. 1983; Delayed de novo methylation in teratocarcinoma cells suggests additional tissue-specific mechanism for controlling gene expression. Nature, London 301:32–37
    [Google Scholar]
  13. Gönczöl E., Andrews P. W., Plotkin S. A. 1984; Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224:159–161
    [Google Scholar]
  14. Katinka M., Vasseur M., Montreau N., Yaniv M., Blangy D. 1981; Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells. Nature, London 290:720–722
    [Google Scholar]
  15. Lang D. J., Montagnier L., Latarjet R. 1974; Growth in agarose of human cells infected with cytomegalovirus. Journal of Virology 14:327–332
    [Google Scholar]
  16. Linnenbach A., Huebner K., Croce C. M. 1981; Transcription of simian virus 40 genome in DNA transformed murine teratocarcinoma stem cells. Proceedings of the National Academy of Sciences, U S,. A 78:6386–6390
    [Google Scholar]
  17. Martin G. R. 1980; Teratocarcinomas and mammalian embryogenesis. Science 209:768–776
    [Google Scholar]
  18. Matthaei K. J., Andrews P. W., Bronson D. L. 1983; Retinoic acid fails to induce differentiation in human teratocarcinoma cell lines that express high levels of cellular receptor protein. Experimental Cell Research 143:471–474
    [Google Scholar]
  19. Montagnier L. 1968; Correlation entre la transformation de cellules BHK-21 et leur resistance aux polysaccharides acides en milieu gelite. Comptes rendus hebdomadaires des seances de l’Academie des sciences, serie D 267:921–924
    [Google Scholar]
  20. Nelson-Rees W. A., Flandermeyer R. R. 1976; HeLa cultures defined. Science 191:96–98
    [Google Scholar]
  21. Pattillo R. A., Gey G. O. 1968; The establishment of a cell line of human hormone synthesizing trophoblastic cells in vitro. Cancer Research 28:1231–1236
    [Google Scholar]
  22. Pattillo R. A., Ruckert A., Hussa R., Bernstein R., Delfs E. 1971; The Jar cell line -continuous human multihormone production and controls. In Vitro 6:398–399
    [Google Scholar]
  23. Schwartzendruber D. E., Lehman J. M. 1975; Neoplastic differentiation: interaction of simian virus 40 and polyoma virus with murine teratocarcinoma cells in vitro. Journal of Cellular Physiology 85:179–187
    [Google Scholar]
  24. Segal S. A., Levine A. J., Khoury G. 1979; Evidence for nonspliced SV40 RNA in undifferentiated murine teratocarcinoma stem cells. Nature, London 280:335–337
    [Google Scholar]
  25. Shevinsky L. H., Knowles B. B., Damjanov I., Solter D. 1982; Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen on mouse embryos and human teratocarcinoma cells. Cell 30:697–705
    [Google Scholar]
  26. Solter D., Knowles B. B. 1978; Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proceedings of the National Academy of Sciences, U.S.A 75:5565–5569
    [Google Scholar]
  27. Teich N. M., Weiss R. A., Martin G. R., Lowy D. R. 1977; Virus infection of murine teratocarcinoma stem cell lines. Cell 12:973–982
    [Google Scholar]
  28. Vaczi L., Gonczol E. 1973; The effect of cytosine arabinosideon the multiplication of cytomegalovirus and on the formation of virus-induced intracellular antigens. Acta virologica 17:189–195
    [Google Scholar]
  29. Weller T. H. 1971; The cytomegaloviruses: ubiquitous agents with protein clinical manifestations. New England Journal of Medicine 285:203–241
    [Google Scholar]
  30. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-3-509
Loading
/content/journal/jgv/10.1099/0022-1317-66-3-509
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error