The interaction of feline leukaemia viruses (FeLV) with erythrocytes was investigated. Haemadsorption (HAd) was observed on the surface of feline embryonic fibroblast cells infected with FeLV. HAd was detected in various degrees when cat, hamster or horse erythrocytes were incubated with cells infected with viruses of subgroup C (FeLV-C) and on cells infected with some FeLV subgroup A viruses (FeLV-A), but not on cells infected with FeLV subgroup B viruses (FeLV-B). HAd of sheep erythrocytes was detected on cells infected with some FeLV-C viruses. The HAd of hamster erythrocytes on cells infected with FeLV-C/Sarma virus was inhibited by antisera against gp70 or p15(E) but not by sera to the other FeLV structural polypeptides. HAd inhibition was also exhibited by cat sera which had FeLV-neutralizing activity but not by sera of specific pathogen-free cats. Haemagglutination by FeLV-C viruses was demonstrated after the virus was treated with neuraminidase and phospholipase C, or Tween-80 and ether. Contrary to expectations from the pattern observed by HAd, all FeLV-A viruses had similar haemagglutinin (HA) activity to FeLV-C viruses. FeLV-B viruses did not possess an HA.
HunsmannG.,
MoennigV.,
PfisterL.,
SeifertE.,
SchaferW.1974; Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering, and hemagglutinating capacities. Virology 62:307–318
JarrettO.1980; Natural occurrence of subgroups of feline leukemia virus. In Viruses in Naturally Occurring Cancers (Cold Spring Harbor Conferences on Cell Proliferation, vol. 7), pp 603–611 Edited by
EssexM.,
TodaroG.,
zur HausenH.
New York: Cold Spring Harbor Laboratory;
NeilJ. C.,
OnionsD. E.,
JarrettO.1980; Polypeptides of feline leukaemia virus: identification of p15(E) and pi 2(E). Journal of General Virology 50:455–460
OnionsD.,
JarrettO.,
TestaN.,
FrassoniF.,
TothS.1982; Selective effect of feline leukaemia virus on early erythroid precursors. Nature, London 296:156–158
SchäferW.,
HunsmannG.,
MoennigV.,
De NoronhaF.,
BolognesiD. P.,
GreenR. W.,
HuperG.1975; Polypeptides of mammalian oncornaviruses. II. Characterization of a murine leukemia virus polypeptide (pi5) bearing interspecies reactivity. Virology 64:48–59
SchaferW.,
FischingerP. J.,
CollinsJ. J.,
BolognesiD. P.1977; Role of carbohydrate in biological functions of Friend murine leukemia virus gp71. Journal of Virology 21:35–40
SchneiderJ.,
SchwarzH.,
HunsmannG.1979; Rosettes from Friend leukemia virus envelope: preparation and physicochemical and partial biological characterization. Journal of Virology 29:624–632
VedbratS. S.,
RasheedS.,
LutzH.,
GondaM. A.,
RuscettiS.,
GardnerM. B.,
PrenskyW.1983; Feline oncornavirus-associated cell membrane antigen: a viral and not a cellularly coded transformation-specific antigen of cat lymphomas. Virology 124:445–461
WitteO. N.,
Tsukamoto-AdeyA.,
WeissmanI. L.1977; Cellular maturation of oncornavirus glycoproteins: topological arrangement of precursor and product forms in cellular membranes. Virology 76:539–553