1887

Abstract

SUMMARY

The protease inhibitor leupeptin prevented multiplication of the human coronavirus strain 229E in cultures of MRC-C cells. The IC of leupeptin in plaque reduction tests was 0.4 µg/ml, whereas growth of host cells was unaffected by leupeptin at 50 µg/ml. Inhibition of plaque formation could be prevented by the addition of proteases to the overlay medium. In single-cycle growth experiments, leupeptin reduced virus yield only if added within 2 h of infection, indicating its action on an early stage of virus replication.

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/content/journal/jgv/10.1099/0022-1317-66-2-363
1985-02-01
2022-09-30
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References

  1. Andersen K. B. 1983; Leupeptin inhibits retrovirus infection in mouse fibroblasts. Journal of Virology 48:765–769
    [Google Scholar]
  2. Aoyagi T., Umezawa H. 1975; Structures and activities of protease inhibitors of microbial origin. In Cold Spring Harbor Conferences on Cell Proliferation vol 2: Proteases and Biological Control, pp 429–453 Edited by Reich E., Rifkin D. B., Shaw E. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  3. Otsuki K., Tsubokura M. 1981; Plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin. Achives of Virology 70:315–320
    [Google Scholar]
  4. Siddell S., Wege H., Ter meulen V. 1983; The biology of coronaviruses. Journal of General Virology 64:761–776
    [Google Scholar]
  5. Storz J., Rott R., Kaluza G. 1981; Enhancement of plaque formation and cell fusion of an enteropathogenic coronavirus by trypsin treatment. Infection and Immunity 31:1214–1222
    [Google Scholar]
  6. Toth T. E. 1982; Trypsin-enhanced replication of neonatal calf diarrhea coronavirus in bovine embryonic lung cells. American Journal of Veterinary Research 43:967–972
    [Google Scholar]
  7. Yoshikura H., Tejima S. 1981; Role of protease in mouse hepatitis virus-induced cell fusion. Studies with a cold-sensitive mutant isolated from a persistent infection. Virology 113:503–511
    [Google Scholar]
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