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Abstract

Summary

Neutralizing monoclonal antibodies incorporated into plaque assay overlay medium were used to select antibody-resistant (Ab) mutants of both the Herts (using antifusion protein monoclonal 481) and Beaudette C (using anti-haemagglutinin-neuraminidase protein monoclonal 445) strains of Newcastle disease virus at the permissive temperature of 34 °C. Certain of the Herts, but none of the Beaudette C, Ab mutants were also temperature-sensitive ( ) and failed to form plaques at the non-permissive temperature of 41.5 °C. [S]Methionine-labelled proteins from chick embryo fibroblasts infected with wild-type, Ab and Ab virus when separated by two-dimensional polyacrylamide gel electrophoresis revealed a variety of changes in the isoelectric point of the fusion protein F (using monoclonal 481) and the haemagglutinin-neuraminidase protein HN (using monoclonal 445). The ‘revertants’ of Ab mutants remained Ab and also showed changed isoelectric points in the F protein.

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/content/journal/jgv/10.1099/0022-1317-66-2-357
1985-02-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-2-357
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