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Abstract
Neutralizing monoclonal antibodies incorporated into plaque assay overlay medium were used to select antibody-resistant (AbR) mutants of both the Herts (using antifusion protein monoclonal 481) and Beaudette C (using anti-haemagglutinin-neuraminidase protein monoclonal 445) strains of Newcastle disease virus at the permissive temperature of 34 °C. Certain of the Herts, but none of the Beaudette C, AbR mutants were also temperature-sensitive (ts −) and failed to form plaques at the non-permissive temperature of 41.5 °C. [35S]Methionine-labelled proteins from chick embryo fibroblasts infected with wild-type, ts + AbR and ts − AbR virus when separated by two-dimensional polyacrylamide gel electrophoresis revealed a variety of changes in the isoelectric point of the fusion protein F (using monoclonal 481) and the haemagglutinin-neuraminidase protein HN (using monoclonal 445). The ts + ‘revertants’ of ts − AbR mutants remained AbR and also showed changed isoelectric points in the F protein.
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