1887

Abstract

SUMMARY

Neutralizing monoclonal antibodies incorporated into plaque assay overlay medium were used to select antibody-resistant (Ab) mutants of both the Herts (using antifusion protein monoclonal 481) and Beaudette C (using anti-haemagglutinin-neuraminidase protein monoclonal 445) strains of Newcastle disease virus at the permissive temperature of 34 °C. Certain of the Herts, but none of the Beaudette C, Ab mutants were also temperature-sensitive ( ) and failed to form plaques at the non-permissive temperature of 41.5 °C. [S]Methionine-labelled proteins from chick embryo fibroblasts infected with wild-type, Ab and Ab virus when separated by two-dimensional polyacrylamide gel electrophoresis revealed a variety of changes in the isoelectric point of the fusion protein F (using monoclonal 481) and the haemagglutinin-neuraminidase protein HN (using monoclonal 445). The ‘revertants’ of Ab mutants remained Ab and also showed changed isoelectric points in the F protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-2-357
1985-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/2/JV0660020357.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-2-357&mimeType=html&fmt=ahah

References

  1. Chambers P., Samson A. C. R. 1982; Non-structural proteins in Newcastle disease virus-infected cells. Journal of General Virology 58:1–12
    [Google Scholar]
  2. Dimmock N. J. 1984; Mechanisms of neutralization of animal viruses. Journal of General Virology 65:1015–1022
    [Google Scholar]
  3. Emini E. A., Jameson B. A., Wimmer E. 1983; Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature, London 304:699–703
    [Google Scholar]
  4. Granoff A. 1959; Studies on mixed infections with Newcastle disease virus. I. Isolation of Newcastle disease mutants and test for genetic recombination between them. Virology 9:636–648
    [Google Scholar]
  5. Harper D. R., Samson A. C. R., Lee C. M., Simon E. 1983; A temperature-sensitive mutant of Newcastle disease virus which is affected in both haemagglutinin-neuraminidase and matrix proteins. Journal of General Virology 64:2781–2784
    [Google Scholar]
  6. Russell P. H., Griffiths P. C., Goswami K. K. A., Alexander D. J., Cannon M. J., Russell W. C. 1983; The characterization of monoclonal antibodies to Newcastle disease virus. Journal of General Virology 64:2069–2072
    [Google Scholar]
  7. Samson A. C. R., Chambers P., Lee C. M., Simon E. 1981; Temperature-sensitive mutant of Newcastle disease virus which has an altered nucleocapsid-associated protein. Journal of General Virology 54:197–201
    [Google Scholar]
  8. Varsanyi T. M., Utter G., Norrby E. 1984; Purification, morphology and antigenic characterization of measles virus envelope components. Journal of General Virology 65:355–366
    [Google Scholar]
  9. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigen variation. Nature, London 289:373–378
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-2-357
Loading
/content/journal/jgv/10.1099/0022-1317-66-2-357
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error