Neutralizing monoclonal antibodies incorporated into plaque assay overlay medium were used to select antibody-resistant (Ab) mutants of both the Herts (using antifusion protein monoclonal 481) and Beaudette C (using anti-haemagglutinin-neuraminidase protein monoclonal 445) strains of Newcastle disease virus at the permissive temperature of 34 °C. Certain of the Herts, but none of the Beaudette C, Ab mutants were also temperature-sensitive ( ) and failed to form plaques at the non-permissive temperature of 41.5 °C. [S]Methionine-labelled proteins from chick embryo fibroblasts infected with wild-type, Ab and Ab virus when separated by two-dimensional polyacrylamide gel electrophoresis revealed a variety of changes in the isoelectric point of the fusion protein F (using monoclonal 481) and the haemagglutinin-neuraminidase protein HN (using monoclonal 445). The ‘revertants’ of Ab mutants remained Ab and also showed changed isoelectric points in the F protein.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error