Isolation of a Mutant of Cowpea Mosaic Virus which is Unable to Grow in Cowpeas Free

Abstract

SUMMARY

Following nitrous acid mutagenesis of cowpea mosaic virus (CPMV) virion RNA, a mutant has been isolated which is able to grow in but is unable to grow in the ‘Early Red’ or ‘Blackeye Early Ramshorn’ varieties of cowpea (). Dot blot analysis failed to detect virus-specific RNA in ‘Blackeye Early Ramshorn’ plants which had been inoculated with mutant 7A-4, indicating that no CPMV RNA synthesis had occurred. Supplementation experiments showed that the mutation was located in B RNA.

Keyword(s): B RNA , CPMV and host range mutant
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-2-339
1985-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/2/JV0660020339.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-2-339&mimeType=html&fmt=ahah

References

  1. Daubert S. D., Bruening G., Najarian R. C. 1978; Protein bound to the genome RNAs of cowpea mosaic virus. European Journal of Biochemistry 92:45–51
    [Google Scholar]
  2. De Jager C. P. 1976; Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests. Virology 70:151–163
    [Google Scholar]
  3. De Jager C. P., Wesseling J. B. M. 1981; Spontaneous mutations in cowpea mosaic virus overcoming resistance due to hypersensitivity in cowpea. Physiological Plant Pathology 19:347–358
    [Google Scholar]
  4. Dorssers L., Van Der Krol S., Van Der Meer J., Van Kammen A., Zabel P. 1984; Purification of Cowpea mosaic virus RNA replication complex: identification of a virus-encoded 110, 000-dalton polypeptide responsible for RNA chain elongation. Proceedings of the National Academy of Sciences, U.S.A 81:1951–1955
    [Google Scholar]
  5. Franssen H., Goldbach R., Broekhuissen M., Moerman M., Van Kammen A. 1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  6. Franssen H., Leunissen J., Goldbach R., Lomonossoff G., Zimmern D. 1984; Homologous sequences in non-structural proteins from cowpea mosaic virus and picornaviruses. EMBO Journal 3:855–861
    [Google Scholar]
  7. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
    [Google Scholar]
  8. Goldbach R. W., Schilthuls J. G., Rezelman G. 1981; Comparison of in vivo and in vitro translation of cowpea mosaic virus RNAs. Biochemical and Biophysical Research Communications 99:89–94
    [Google Scholar]
  9. Gopo J. M., Frist R. H. 1977; Location of the gene specifying the smaller protein of the cowpea mosaic virus capsid. Virology 79:259–266
    [Google Scholar]
  10. Lomonossoff G. P., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
    [Google Scholar]
  11. Pelham H. R. B. 1979; Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology 96:463–477
    [Google Scholar]
  12. Rezelman G., Goldbach R., Van Kammen A. 1980; Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. Journal of Virology 36:366–371
    [Google Scholar]
  13. Rottier P. J. M., Rezelman G., Van Kammen A. 1980; Protein synthesis in cowpea mosaic virus-infected cowpea protoplasts: detection of virus-related proteins. Journal of General Virology 51:359–371
    [Google Scholar]
  14. Stanley J., Goldbach R., Van Kammen A. 1980; The genome-linked protein of cowpea mosaic virus is coded by RNA from the bottom component. Virology 106:180–182
    [Google Scholar]
  15. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:633–642
    [Google Scholar]
  16. Van Kammen A. 1968; The relationship between the components of cowpea mosaic virus. 1. Two ribonucleoprotein particles necessary for the infectivity of CPMV. Virology 34:312–318
    [Google Scholar]
  17. Van Kammen A., De Jager C. P. 1978; Cowpea mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 197
    [Google Scholar]
  18. Zabel P., Moerman M., Lomonossoff G. P., Shanks M., Beyreuther K. 1984; Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA. EMBO Journal 3:1629–1634
    [Google Scholar]
  19. Zimmern D. 1975; The 5′ end group of tobacco mosaic virus RNA is m7G5ppp5 Gp. Nucleic Acids Research 2:1189–1201
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-2-339
Loading
/content/journal/jgv/10.1099/0022-1317-66-2-339
Loading

Data & Media loading...

Most cited Most Cited RSS feed