1887

Abstract

SUMMARY

The effect of interferon (IFN) on infection and maintenance of persistent infection of Borna disease (BD) virus in cell cultures was investigated. Acutely BD virus-infected primary rabbit brain and rat lung cells produced significant levels of interferon detectable 3 days post-infection in the culture supernatants. Rat brain and rat lung cells persistently infected with BD virus produced only moderate levels of IFN over a long period. In contrast, persistently infected Madin-Darby canine kidney (MDCK) cells did not produce detectable amounts of IFN. Exogenous homologous IFN completely inhibited the expression of BD virus antigen in acutely infected rabbit brain cells, when added during the first 24 h after infection. IFN added later (2 to 6 days post-infection) reduced virus titres to different degrees depending on the onset of treatment. However, IFN added to persistently infected rat lung cells did not appear to influence the degree or quality of BD virus antigen expression or the intracellular amount of infectious virus. Two facts indicate that IFN is not involved in the establishment or maintenance of persistent BD virus infection . Thus, MDCK cells, which could not be induced to produce IFN, can be readily persistently infected with BD virus , and exogenous IFN did not appear to influence persistent BD virus infection.

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1985-12-01
2024-12-08
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References

  1. Burke D. C., Isaacs A. 1958; Some factors affecting the production of interferon. British Journal of Experimental Pathology 39:452–458
    [Google Scholar]
  2. Danner K. 1982; Borna Virus and Borna-Infektion. com Miasma zum Modell. Stuttgart: Ferdinand Enke Verlag.
    [Google Scholar]
  3. Finter N. B. 1965; A comparison of the amounts of mouse interferon obtained from different sources. Nature, London 206:597–599
    [Google Scholar]
  4. Gandhi S. S., Burke D. C., Scholtissek C. 1970; Virus RNA synthesis by ultraviolet-irradiated Newcastle disease virus and interferon production. Journal of General Virology 9:97–99
    [Google Scholar]
  5. Henderson D. R., Joklik W. K. 1978; The mechanism of interferon induction by UV-irradiated Reovirus. Virology 91:389–406
    [Google Scholar]
  6. Herzog S., Rott R. 1980; Replication of Borna disease virus in cell cultures. Medical Microbiology and Immunology 168:153–158
    [Google Scholar]
  7. Herzog S., Kompter C., Frese K., Rott R. 1984; Replication of Borna disease virus in rats: age-dependent differences in tissue distribution. Medical Microbiology and Immunology 173:171–177
    [Google Scholar]
  8. Hirt H. M., Becker H., Kirchner H. 1978; Induction of interferon production in mouse spleen cell cultures by Corynebacterium parrum. Cellular Immunology 38:168–175
    [Google Scholar]
  9. Narayan O., Herzog S., Frese K., Scheefers H., Rott R. 1983a; Behavioral disease in rats caused by immunopathological responses to persistent Borna virus in the brain. Science 220:1401–1403
    [Google Scholar]
  10. Narayan O., Herzog S., Frese K., Scheefers H., Rott R. 1983b; Pathogenesis of Borna disease in rats: immune-mediated viral ophthalmoencephalopathy causing blindness and behavioral abnormalities. Journal of Infectious Diseases 148:305–315
    [Google Scholar]
  11. Stewart W. E. II 1979 The Interferon System Wien & New York: Springer-Verlag;
    [Google Scholar]
  12. Vallbracht A., Hofmann L., Wurster K. G., Flehmig B. 1984; Persistent infection of human fibroblasts by hepatitis A virus. Journal of General Virology 65:609–615
    [Google Scholar]
  13. Zwick W. 1939; Bornasche Krankheit und Encephalomyelitis der Tiere. In Handbuch der Viruskrankheiten vol 2: pp 254–356 Edited by Gildemeister F., Haagen E., Waldmann O. Jena: Gustav Fischer Verlag;
    [Google Scholar]
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