1887

Abstract

SUMMARY

Mammalian and arthropod cell cultures were used to assess the neutralizing activity of six monoclonal antibodies specific for the G1 glycoprotein of La Crosse virus. Four antibodies, two neutralizing and two non-neutralizing, showed no host-dependent differences, giving similar results when post-treatment infectivity was determined using either cells or BHK-21 cells. For two other antibodies, however, dissimilar activities were observed between the vertebrate and invertebrate cell lines. One of these antibodies was positive when BHK-21 cells were employed as the post-treatment host and negative when mosquito cells were used; the other antibody was the converse. The epitope for this last antibody was present on all California serogroup viruses examined, which suggests that it may have a special significance in the natural life-cycle of the virus.

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/content/journal/jgv/10.1099/0022-1317-66-12-2773
1985-12-01
2022-09-24
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References

  1. Davey M. W., Dennett D. P., Dalgarno L. 1973; The growth of two togaviruses in cultured mosquito and vertebrate cells. Journal of General Virology 20:225–232
    [Google Scholar]
  2. Gentsch J. R., Bishop D. H. L. 1978; Small viral RNA segment of bunyaviruses codes for viral nucleocapsid protein. Journal of Virology 28:417–419
    [Google Scholar]
  3. Gentsch J. R., Bishop D. H. L. 1979; M viral RNA segment of bunyaviruses codes for two glycoproteins, G1 and G2. Journal of Virology 30:767–770
    [Google Scholar]
  4. Gonzalez-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1982; Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunyaviruses. Virology 120:42–53
    [Google Scholar]
  5. Grady L. J., Srihongse S., Grayson M. A., Deibel R. 1983a; Monoclonal antibodies against La Crosse virus. Journal of General Virology 64:1699–1704
    [Google Scholar]
  6. Grady L. J., Sanders M. L., Campbell W. P. 1983b; Evidence for three separate antigenic sites on the G1 protein of La Crosse virus. Virology 126:395–397
    [Google Scholar]
  7. Grimstad P. R. 1983; Mosquitoes and the incidence of encephalitis. Advances in Virus Research 28:357–438
    [Google Scholar]
  8. Kingsford L., Hill D. W. 1981; The effects of proteolytic enzymes on structure and function of La Crosse G1 and G2 glycoproteins. In The Replication of Negative Strand Viruses pp 111–116 Edited by Compans R. W., Bishop D. H. L. New York & London: Academic Press;
    [Google Scholar]
  9. Kingsford L., Ishizawa L. D., Hill D. W. 1983; Biological activities of monoclonal antibodies reactive with antigenic sites mapped on the G1 glycoprotein of La Crosse virus. Virology 129:443–455
    [Google Scholar]
  10. Lyons M. J., Heyduk J. 1973; Aspects of the developmental morphology of California encephalitis virus in cultured vertebrate and arthropod cells and mouse brain. Virology 54:37–52
    [Google Scholar]
  11. Mitsuhashi J., Maramorosch K. 1964; Leafhopper tissue culture: embryonic, nymphal, and imaginal tissues for aseptic insects. Contributions. Boyce Thompson Institute for Plant Research 22:435–460
    [Google Scholar]
  12. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976a; Structural proteinsof La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  13. Obijeski J. F., Bishop D. H. L., Palmer E. L., Murphy F. A. 1976b; Segmented genome and nucleocapsid of La Crosse virus. Journal of Virology 20:664–675
    [Google Scholar]
  14. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. YA., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Oker-Blom N., Russell P. K. 1975/76; Bunyaviruses and Bunyaviridae. Intervirology 6:13–24
    [Google Scholar]
  15. Thompson W. H., Kalfayan B., Anslow R. O. 1965; Isolation of California encephalitis group virus from a fatal human illness. American Journal of Epidemiology 81:245–253
    [Google Scholar]
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