1887

Abstract

SUMMARY

Evidence is presented for the production in dengue type 2 virus (DEN-2)-infected BHK cells of large virus-specific proteins with molecular weights up to 250000. These proteins were most prominent in lysates of cells which had been labelled with [S]methionine for 7 to 15 min. During pulse-chase experiments, these high mol. wt. proteins appeared to be converted into smaller, more stable, proteins with mol. wt. between 10000 and 98000. Finally, inhibition of proteolysis prevented the chasing of label from the high mol. wt. proteins to the smaller viral proteins which normally accumulate in DEN-2-infected cells. These findings are consistent with the idea that processing of large polyprotein precursors plays an important role in the production of flavivirus proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-12-2767
1985-12-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/12/JV0660122767.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-12-2767&mimeType=html&fmt=ahah

References

  1. Baltimore D. 1971; Expression of animal virus genomes. Bacteriological Reviews 35:235–241
    [Google Scholar]
  2. Boulton R. W., Westaway E. G. 1977; Togavirus RNA: reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells. Archives of Virology 55:201–208
    [Google Scholar]
  3. Butter worth B. E., Korant B. D. 1974; Characterization of the large picornaviral proteins produced in the presence of zinc ion. Journal of Virology 14:282–291
    [Google Scholar]
  4. Cleaves G. R., Dubin D. T. 1979; Methylation status of intracellular dengue type 2 40S RNA. Virology 96:159–165
    [Google Scholar]
  5. Cleaves G. R., Schlesinger R. W. 1977; Characterization of polysomal RNA’s from dengue virus infected KB cells. Abstracts of the Annual Meeting of the American Society for Microbiology p 287
    [Google Scholar]
  6. Cleaves G. R., Ryan T. E., Schlesinger R. W. 1981; Identification and characterization of dengue type 2 virus replicative intermediate and replicative form RNA’s. Virology 111:73–83
    [Google Scholar]
  7. Korant B. D. 1972; Cleavage of viral precursor proteins in vivo and in vitro. Journal of Virology 10:751–759
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Margolis J., Kendrick K. G. 1968; Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Analytical Biochemistry 25:347–362
    [Google Scholar]
  10. Naeve D. W., Trent D. W. 1978; Identification of St. Louis encephalitis virus mRNA. Journal of Virology 25:535–545
    [Google Scholar]
  11. Rice C. M., Lenches E. M., Eddy S. R., Shin S.-L., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  12. Stollar V., Thomas V. 1975; An agent in the A. aegypti cell line (Peleg) which causes fusion in A. albopictus cells. Virology 64:367–371
    [Google Scholar]
  13. Svitkin Y. U., Ugarova T. Y., Chernovskaya T. V., Lyapustin V. N., Lashkevich V. A., Agol V. I. 1981; Translation of tick-borne encephalitis virus (flavivirus) genome in vitro: synthesis of two structural polypeptides. Virology 110:26–34
    [Google Scholar]
  14. Wengler G., Beato M., Wengler G. 1979; In vitro translation of 42S virus-specific RNA from cells infected with the flavivirus West Nile virus. Virology 96:516–529
    [Google Scholar]
  15. Westaway E. G. 1977; Strategy of the flavivirus genome: evidence for multiple internal initiation of proteins specified by Kunjin virus in mammalian cells. Virology 80:320–335
    [Google Scholar]
  16. Westaway E. G. 1980; Replication of flaviviruses. In The Togaviruses: Biology, Structure and Replication pp 531–581 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  17. Westaway E. G., Speight G., Endo L. 1984; Gene order of translation of the flavivirus Kunjin: further evidence of internal initiation in vivo. Virus Research 1:333–350
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-12-2767
Loading
/content/journal/jgv/10.1099/0022-1317-66-12-2767
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error