Comparison of the primary structures of eggplant mosaic virus (EMV) and turnip yellow mosaic virus (TYMV) coat proteins shows that 32% of their amino acids are conserved. Alignment of the two sequences requires only one deletion near the N terminus and two insertions at the C terminus of TYMV coat protein. Although the coat protein of EMV is on average less hydrophobic than that of TYMV, structural predictions yield fairly similar conformations for the two proteins, apart from the N terminus. Neither coat protein possesses an accumulation of basic residues able to form the strong ionic RNA-protein interactions observed in serveral other isometric viruses. The nature of the amino acid exchanges seems to be different from that seen in families of homologous proteins. The highly conserved regions encompass a (probably weak) potential RNA-protein interaction site. Implications for the structure and stability of small isometric viruses are discussed.
BouleyJ. P.,
BriandJ. P.,
WitzJ.1977; The stability of eggplant mosaic virus: action of urea and alkaline pH on top and bottom components. Virology 78:425–432
DayhoffM. O.,
SchwartzR. M.,
OrcuttB. C.1978; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure vol 5: supplement 3 pp 345–352 Edited by
DayhoffM. O.
Washington, D.C.: NBR Foundation, Georgetown University;
DupinA.,
PeterR.,
CollotD.,
DasB. C.,
PeterC.,
BouillonP.,
DurantonH.1984; The primary structure of the eggplant mosaic virus (EMV) coat protein. Comptes rendus hebdomadaires des seances de I’Academie des sciences, séerie C 298:219–221
EhresmannB.,
BriandI. P.,
ReinboltJ.,
WitzJ.1980; Identification of binding sites of turnip yellow mosaic virus protein and RNA by crosslinks induced in situ. European Journal of Biochemistry 108:123–129
HélèneC.,
LancelotG.1982; Interactions between functional groups in protein-nucleic acid associations. Progress in Biophysics and Molecular Biology 39:1–68
HoppT. P.,
WoodsK. R.1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences, U. S. A 78:3824–3828
KaperJ. M.1975The Chemical Basis of Virus Structure, Dissociation and Reassembly. Frontiers of Biology series Edited by
NeubergerA.,
TatumE. L.
Amsterdam: North-Holland;
KoenigR.,
LesemannD. E.1981; Tymoviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 33–60 Edited by
KurstakE.
Amsterdam: Elsevier/North-Holland;
PaulH. L.,
GibbsA.,
Wittman-LieboldB.1980; The relationship of certain tymoviruses assessed from the amino acid composition of their coat protiens. Intervirology 13:99–109
PrattD.,
BriandJ. P.,
Van RegenmortelM. H. V.1980; Immunochemical studies of turnip yellow mosaic virus. I. Localization of four antigenic regions in the protein subunit. Molecular Immunology 17:1167–1171
QuesniauxV.,
BriandJ. P.,
Van RegenmortelM. H. V.1983a; Immunochemical studies of turnip yellow mosaic virus. II. Localization of a viral epitope in the N-terminal residues of the coat protein. Molecular Immunology 20:179–185
QuesniauxV.,
JaegleM.,
Van RegenmortelM. H. V.1983b; Immunochemical studies of turnip yellow mosaic virUS. III. Localization of two viral epitopes in residues 57–64 and 183–189 of the coat protein. Biochimica et Biophysica acta 743:226–231
RacanielloV.,
BaltimoreD.1981; Molecular cloning of poliovirus cDN A and determination of the complete nucleotide sequence of the viral genome. Proceedings of the National Academy of Sciences, U. S. A 78:4887–4891
RiceD. M.,
SchulzG. E.,
GuestJ. R.1984; Structural relationship between glutathione reductase and lipoamide dehydrogenase. Journal of Molecular Biology 174:483–496
RossmannM. G.,
Abad-ZapateroC.,
MurthyM. R.,
LiljasK.,
JonesA.,
StrandbergB.1983; Structural comparisons of some small spherical viruses. Journal of Molecular Biology 165:711–736
SauerR. T.,
YocumR. R.,
DoolittleR. F.,
LewisM.,
PaboC. O.1982; Homology among DNA-binding proteins suggests use of a conserved super-secondary structure. Nature, London 298:447–451
SchifferM.,
EdmundsonA. B.1967; Use of helical wheels to represent the structures of proteins and to identify segments with helical potential. Biophysical Journal 7:121–135
SchwartzR. M.,
DayhoffM. O.1978; Matrices for detecting distant relationships. In Atlas of Protein Sequence and Structure vol 5: supplement 3 pp 353–359 Edited by
DayhoffM. O.
Washington, D.C.: NBR Foundation, Georgetown University;
StubbsG.,
WarrenS.,
HolmesK. C.1977; Structure of RNA and RNA binding site in tobacco mosaic virus from 4 A map calculated from X-ray fibre diagrams. Nature, London 267:216–221
VirudachalamR.,
SitaramanK.,
HeussK. L.,
ArgosP.,
MarkleyJ. L.1983; Carbon-13 and proton nuclear magnetic resonance spectroscopy of plant viruses: evidence for protein-nucleic acid interactions in belladonna mottle virus and detection of polyamines in turnip yellow mosaic virus. Virology 130:360–371
WesthoffE.,
AltschuhD.,
MorasD.,
BloomerA. C.,
MondragonA.,
KlugA.,
Van RegenmortelM. H. V.1984; Correlation between segmental mobility and the location of antigenic determinants in proteins. Nature, London 311:123–126