1887

Abstract

SUMMARY

Monoclonal and polyclonal antibodies with known specificity for either the 54K envelope glycoprotein or the 48K non-structural glycoprotein of yellow fever (YF) virus-infected cells were studied in plaque reduction neutralization tests. Viruses employed in the tests comprised wild-type and vaccine strains of YF and a selection of other flaviviruses. Of 17 monoclonal antibodies examined, six of the 54K-specific antibodies neutralized at least one YF preparation. Both vaccine and wild-type YF viruses varied in their susceptibility to neutralization and there were also differences between individual 17D vaccine strains. The monoclonal antibodies produced a range of titres with the different viruses, the most potent, 864, leaving no non-neutralizable fraction. Addition of anti-globulin, complement or other antibodies did not affect the results. YF-neutralizing antibodies which bound to other flaviviruses did not necessarily neutralize them; hence, neutralization could be defined as either homotypic, heterotypic or both homotypic and heterotypic. A polyclonal antiserum and a broadly reacting monoclonal antibody produced almost identical neutralization results in tests with wild-type YF viruses. In contrast, the polyclonal antiserum produced higher titres with vaccine strains of YF. In mouse passive protection experiments on the other hand, the monoclonal antibody did not differentiate between these viruses.

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1985-12-01
2022-08-10
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References

  1. Atassi M. Z. 1980; Precise determination of protein antigenic structures has unravelled the molecular immune recognition of proteins and provided a prototype for synthetic mimicking of other protein binding sites. Molecular & Cellular Biochemistry 32:21–43
    [Google Scholar]
  2. Bradish C. J., Farley J. O., Ferrier H. E. N. 1962; Studies of the nature of the neutralisation reaction and the competition for neutralising antibody between components of the virus system of foot and mouth disease. Virology 18:378–400
    [Google Scholar]
  3. Brown F. 1985 Classification and Nomenclature of Viruses 5th Report Intervirology (in press)
    [Google Scholar]
  4. Chanas A. C., Gould E. A., Clegg J. C. S., Varma M. G. R. 1982; Monoclonal antibodies to Sindbis virus glycoprotein E1 can neutralize, enhance infectivity, and independently inhibit haemagglutination or haemolysis. Journal of General Virology 58:37–46
    [Google Scholar]
  5. Della-porta A. J., Westaway E. G. 1978; A multi-hit model for the neutralization of animal viruses. Journal of General Virology 38:1–19
    [Google Scholar]
  6. Fenner F., Pereira H. G., Porterfield J. S., Joklik W. K., Downie A. W. 1974; Family and generic names for viruses approved by the International Committee on Taxonomy of Viruses. Intervirology 3:193–198
    [Google Scholar]
  7. Fitzgeorge R., Bradish C. J. 1980; The in vivo differentiation of strains of yellow fever virus in mice. Journal of General Virology 46:1–14
    [Google Scholar]
  8. Flamand A., Wiktor T. J., Koprowski H. 1980; Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. II. The glycoprotein. Journal of General Virology 48:105–109
    [Google Scholar]
  9. Gould E. A., Buckley A., Cammack N., Barrett A. D. T., Clegg J. C. S., Ishak R., Varma M. G. R. 1985; Examination of the immunological relationships between flaviviruses using yellow fever virus monoclonal antibodies. Journal of General Virology 66:1369–1382
    [Google Scholar]
  10. Heinz F. X., Tuma W., Kunz C. 1981; Antigenic and immunological properties of defined physical forms of tick-borne encephalitis virus structural proteins. Infection and Immunity 33:250–257
    [Google Scholar]
  11. Kingsford L. 1984; Enhanced neutralization of La Crosse virus by the binding of specific pairs of monoclonal antibodies to the G1 glycoprotein. Virology 136:265–273
    [Google Scholar]
  12. Kitano T., Suzuki K., Yamaguchi T. 1974; Morphological, chemical and biological characterization of Japanese encephalitis virus virion and its hemagglutinin. Journal of Virology 14:631–639
    [Google Scholar]
  13. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  14. LiprandI F. 1981; Isolation of plaque variants differing in virulence from the 17D strain of yellow fever virus. Journal of General Virology 56:363–370
    [Google Scholar]
  15. LubecK M. D., Gerhard W. 1982; Conformational changes at topologically distinct antigenic sites on the influenza A/PR/8/34 molecule are induced by the binding of monoclonal antibodies. Virology 118:1–7
    [Google Scholar]
  16. Madrid De A. T., Porterfield J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
    [Google Scholar]
  17. Monath T. P., Schlesinger J. J., Brandriss M. W., Cropp C. B., Prange W. C. 1984; Yellow fever monoclonal antibodies: type specific and cross reactive determinants identified by immunofluorescence. American Journal of Tropical Medicine and Hygiene 33:695–698
    [Google Scholar]
  18. Porterfield J. S. 1980; Antigenic characteristics and classification of Togaviridae. In The Togaviruses pp 13–46 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  19. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralisation, hemagglutination inhibition and immune precipitation of the virus envelope. Virology 125:8–17
    [Google Scholar]
  20. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. 1971; The proteins of Japanese encephalitis virus. Virology 44:108–124
    [Google Scholar]
  21. Varma M. G. R., Pudney M., Leake C. J., Peralta P. H. 1975/76; Isolations in a mosquito Aedes pseudoscutellaris cell line (MOS 61) of yellow fever virus strains from original field material. Intervirology 6:50–56
    [Google Scholar]
  22. Wildy P. 1971; Classification and Nomenclature of Viruses, First Report. Monographs in Virology 5 Basel: S. Karger;
    [Google Scholar]
  23. Woodall J. P. 1981; Summary of a symposium on yellow fever. Journal of Infectious Diseases 144:87–91
    [Google Scholar]
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