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Festuca leaf streak virus (FLSV), a non-sap-transmissible rhabdovirus which has only been found in Festuca gigantea (Gramineae), multiplied in cowpea protoplasts inoculated with virus suspensions containing polyethylene glycol. The morphogenesis of FLSV particles was studied by electron microscopy. FLSV seemed to be assembled in the cytoplasm by simultaneous coiling and envelopment of the nucleocapsid. However, particles detached from the host membrane were not observed. Similarities with the morphogenesis of particles of Sonchus yellow net virus in cowpea protoplasts are discussed.
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