1887

Abstract

SUMMARY

A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using high concentrations of detergent and salt and by differential centrifugation procedures. The protein, present in an aggregated form, was remarkably resistant to proteinase K. Specific antibodies prepared in the homologous system (rat) recognized the 14.5K protein from various sources (infected brain of rat, mouse or chicken, and tissue cultures), but did not neutralize infectivity nor stain Borna disease virus-specific antigens from or preparations. Post-infection immune sera from different animal species did not detect the protein. This 14.5K protein was infection-specific but not disease-specific, and is inferred to be part of an internal virion component.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-11-2479
1985-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/11/JV0660112479.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-11-2479&mimeType=html&fmt=ahah

References

  1. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radio-iodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  2. Danner K., Mayr A. 1979; In vitro studies on Borna virus. II. Properties of the virus. Archives of Virology 61:261–271
    [Google Scholar]
  3. Gosztonyi G., Ludwig H. 1984; Borna disease of horses. An immunohistological and virological study of naturally infected animals. Acta neuropathologica 64:213–221
    [Google Scholar]
  4. Hilmert H., Diringer H. 1984; A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters. Bioscience Reports 4:165–170
    [Google Scholar]
  5. Hirano N., Kao M., Ludwig H. 1983; Persistent, tolerant or subacute infection in Borna disease virus-infected rats. Journal of General Virology 64:1521–1530
    [Google Scholar]
  6. Kao M., Ludwig H., Gosztonyi G. 1984; Adaptation of Borna disease virus to the mouse. Journal of General Virology 65:1845–1849
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Ludwig H., Becht H. 1977; Borna disease -a summary of our present knowledge. In Slow Virus Infections of the Central Nervous System pp 75–83 Edited by Ter Meulen V., Katz M. New York, Heidelberg & Berlin: Springer-Verlag;
    [Google Scholar]
  9. Ludwig H., Koester V., Pauli G., Rott R. 1977; The cerebrospinal fluid of rabbits infected with Borna disease virus. Archives of Virology 55:209–223
    [Google Scholar]
  10. Multhaup G., Diringer H., Hilmert H., Prinz H., Heukeshoven J., Beyreuther K. 1985; The protein component of scrapie-associated fibrils is a glycosylated low-molecular-weight protein. EMBO Journal 4:1495–1501
    [Google Scholar]
  11. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
    [Google Scholar]
  12. Pauli G., Ludwig H. 1985; Increase of virus yields and release of Borna disease virus from persistently infected cells. Virus Research 2:29–33
    [Google Scholar]
  13. Pauli G., Grunmach J., Ludwig H. 1984; Focus immunoassay for Borna disease virus-specific antigens. Zentralblatt fiir Veterinarmedizin B31:552–557
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-11-2479
Loading
/content/journal/jgv/10.1099/0022-1317-66-11-2479
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error