1887

Abstract

SUMMARY

Isometric particles of vicia cryptic virus (VCV), purified from seedlings infected through seed, had a buoyant density in CsCl of 1.37 g/ml. Analysis by PAGE showed that VCV particles contained three species of dsRNA with mol. wt. of 1.37 × 10, 1.26 × 10 and 1.21 × 10. These dsRNA species were also detected in extracts from leaves, stems, roots, flowers, seeds and mesophyll protoplasts of ; dsRNA was readily detected in extracts from one leaflet (about 0.6 g). Slower-migrating species of dsRNA occurred in many leaf extracts but were not obtained from VCV particles. Electrophoresis of dsRNA extracts in gels that are then stained with silver appears to be a simple and reliable method for detecting ‘cryptic’ viruses. Using this method, VCV dsRNA was detected in 18 cultivars of , but not in cultivars Beryl and Minica which were thought from previous work to be free from VCV. Neither VCV particles nor VCV dsRNA were detected in VCV-free individual plants inoculated with VCV mechanically or by dodder. VCV was not detected in leaves of plants affected by cytoplasmic male sterility or in a maintainer line for this condition.

Keyword(s): cryptic virus , dsRNA , protoplasts and vicia
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1985-11-01
2024-04-24
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References

  1. Beier H., Bruening G. 1975; The use of abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. Virology 64:212–276
    [Google Scholar]
  2. Boccardo G., Lisa V., Milne R. G. 1983; Cryptic viruses in plants. In Double-stranded RNA Viruses pp 425–430 Edited by Compans R. W., Bishop D. H. L. Amsterdam & New York: Elsevier;
    [Google Scholar]
  3. Buck K. W. 1983; Current problems in fungal virus taxonomy. In A Critical Appraisal of Viral Taxonomy pp 139–175 Edited by F R. E. Matthews, Florida: CRC Press;
    [Google Scholar]
  4. Grill L. K., Garger S. J. 1981; Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility of Vicia faba. Proceedings of the National Academy of Sciences, U. S. A 78:7043–7046
    [Google Scholar]
  5. Igloi G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
    [Google Scholar]
  6. Jones A. T. 1980; Seed-borne viruses of Vicia faba and the possibility of producing seed free from broad bean stain virus and Echtes Ackerbohnenmosaik-virus. In Vicia faba. Feeding Value, Processing and Viruses pp 319–333 Edited by Bond D. A. The Hague: Martinus Nijhoff;
    [Google Scholar]
  7. Kassanis B., White R. F., Woods R. D. 1977; Beet cryptic virus. Phytopathologische Zeitschrift 90:350–360
    [Google Scholar]
  8. Kenten R. H., Cockbain A. J., Woods R. D. 1978; Vicia cryptic virus. Report of Rothamsted Experimental Station for 1977:222
    [Google Scholar]
  9. Kenten R. H., Cockbain A. J., Woods R. D. 1979; Vicia cryptic virus. Report of Rothamsted Experimental Station for 1978:220–221
    [Google Scholar]
  10. Kenten R. H., Cockbain A. J., Woods R. D. 1980; Vicia cryptic virus (VCV). Report of Rothamsted Experimental Station for 1979:176
    [Google Scholar]
  11. Kenten R. H., Cockbain A. J., Woods R. D. 1981; Vicia cryptic virus (VCV). Report of Rothamsted Experimental Station for 1980:188–189
    [Google Scholar]
  12. Koenig R., Lesemann D. E. 1980; Two isometric viruses in poinsettias. Plant Disease 64:782–784
    [Google Scholar]
  13. Lisa V., Boccardo G., Milne R. G. 1981; Double stranded ribonucleic acid from carnation cryptic virus. Virology 115:410–413
    [Google Scholar]
  14. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  15. Natsuaki T., Yamashita S., Doi Y., Yora K. 1979; Radish yellow edge virus, a seed-borne small spherical virus newly recognized in Japanese radish (Raphanus sativus L.). Annals of the Phytopathological Society of Japan 45:313–320
    [Google Scholar]
  16. Natsuaki T., Yamashita S., Doi Y., Okuda S., Teranaka M. 1983a; Radish yellow edge virus, a seed-borne virus with double-stranded RNA, of a possible new group. Annals of the Phytopathological Society ofJapan 49:593–599
    [Google Scholar]
  17. Natsuaki T., Yamashita S., Doi Y., Okuda S., Teranaka M. 1983b; Two seed-borne double-stranded RNA viruses, beet temperate virus and spinach temperate virus. Annals of the Phytopathological Society ofJapan 49:709–712
    [Google Scholar]
  18. Natsuaki K. T., Natsuaki T., Okuda S., Teranaka M., Yamashita S., Doi Y. 1984; Two Seed-bome viruses from leguminous plants, alfalfa temperate virus (ATV) and white clover temperate virus (WCTV). Journal of Agricultural Science, Tokyo Nogyo Daigaku 29:49–55
    [Google Scholar]
  19. Reddy D. V. R., Boccardo G., Outridge R., Teakle D. S., Black L. M. 1975; Electrophoretic separation of dsRNA genome segments from Fiji disease and maize rough dwarf viruses. Virology 63:287–291
    [Google Scholar]
  20. Roberts I. M., Harrison B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  21. Sammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135–141
    [Google Scholar]
  22. Sehgal O. P., Jean J., Bhalla R. B., Soong M. M., Krause G. F. 1970; Correlation between buoyant density and ribonucleic acid content in viruses. Phytopathology 60:1778–1784
    [Google Scholar]
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