1887

Abstract

SUMMARY

granulosis viruses (CpGV) from seven different sources in Europe, America and New Zealand were compared by restriction enzyme analysis. Most samples were indistinguishable from the Mexican isolate (CpGV-M). Isolates from Russia (CpGV-R) and England (CpGV-E) showed small genotypic differences. CpGV-E was shown to be a mixture of two variants, E1 and E2. CpGV-E1 was indistinguishable from CpGV-M. A physical map of CpGV-M was constructed for the enzymes RI, HI, dIII, I and I. A comparison of fragment profiles allowed construction of maps for CpGV-R and CpGV-E2. Relative to CpGV-M, CpGV-R had a single deletion of 2.4 kbp and CpGV-E2 was modified in one area resulting in an additional RI site, a shift in a HI site and in total about 1 kbp more DNA. The map was orientated by locating the granulin gene using the cloned granulin gene from GV as a probe. There was no significant difference between the infectivities of the Mexican, Russian and English isolates for neonate larvae.

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1985-11-01
2021-10-25
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