Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. I. Molecular Cloning and Characterization Free

Abstract

SUMMARY

The 78A1 isolate of Moloney murine sarcoma virus (78A1 Mo-MuSV) was cloned from a genomic library obtained from virus producer rat cells, in the lambda vector L47. Among the recombinants hybridizing with a probe specific for the v- sequences, we recovered a recombinant which contained leukaemia virus (MuLV) sequences and was able to transform both mouse and rat cells in transfection experiments. The cloned provirus could be rescued by both Mo-MuLV ecotropic and amphotropic viruses in mouse cells, but only with the amphotropic helper virus in rat cells. Comparative restriction mapping indicates that the 78A1 provirus is 200 bp longer than the HT1 provirus. The difference lies in the junction region of Mo-MuSV. Other minor differences were found in the region, whereas the restriction patterns of the 3′ parts of the proviruses were identical.

Keyword(s): genome , molecular cloning and MuSV
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1985-11-01
2024-03-29
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References

  1. Bernard C., Boiron M., Lasneret J. 1967; Transformation et infection chronique de cellules embryonnaires de rat par le virus du sarcome de Moloney. Compte rendu hebdomadaire des séances de l’Académie des sciences 264:2170–2173
    [Google Scholar]
  2. Blair D. G., McClements W. L., Oskarsson M. K., Fishinger P. J., Vande Woude G. F. 1980; Biological activity of cloned Moloney sarcoma virus DNA : terminally redundant sequences may enhance transformation efficiency. Proceedings of the National Academy of Sciences, U. S. A 77:3504–3508
    [Google Scholar]
  3. Blair D. G., Oskarsson M., Wood T. G., Mcclements W. L., Fischinger P. J., Vande Woude G. F. 1981; Activation of the transforming potential of a normal cell sequence: a molecular model for oncogenesis. Science 212:941–943
    [Google Scholar]
  4. Brow M. A. D., Sen A., Sutcliffe J. G. 1984; Nucleotide sequence of the transforming gene of ml murine sarcoma virus. Journal of Virology 49:579–582
    [Google Scholar]
  5. Devaux J., Buendia M. A., Hamelin R., Bernard C., Tavitian A. 1982; Characterization of large genomic size Moloney murine sarcoma virus produced by a transformed rat cell line. Journal of General Virology 63:223–226
    [Google Scholar]
  6. Donoghue D. J., Hunter T. 1983; Recombinational junctions of variants of Moloney murine sarcomavirus: generation and divergence of a mammalian transforming gene. Journal of Virology 45:607–617
    [Google Scholar]
  7. Donoghue D. J., Sharp P. A., Weinberg R. A. 1979; Comparative study of different isolates of murine sarcoma virus. Journal of Virology 32:1015–1027
    [Google Scholar]
  8. Graham F. L., Van Der Eb A. J. 1973; Transformation of rat cells by DNA of human adenovirus 5. Virology 54:536–539
    [Google Scholar]
  9. Hamelin R., Devaux J., Honoré N., Auger-Buendia M. A., Tavitian A. 1983; Characterization of viral RNA in cells transformed by various isolates of Moloney murine sarcoma virus. Journal of General Virology 64:2057–2062
    [Google Scholar]
  10. Harbers K., Schincke A., Stuhlmann H., Jähner D., Jaenisch R. 1981; DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning. Proceedings of the National Academy of Sciences, U. S. A 78:7609–7613
    [Google Scholar]
  11. Hartley J. W., Rowe W. P. 1976; Naturally occurring murine leukemia viruses in wild mice: characterization of a new amphotropic class. Journal of Virology 19:19–25
    [Google Scholar]
  12. Joseph D. R. 1981; Comparison of the restriction endonuclease maps of unintegrated proviral DNAs from four xenotropic murine leukemia viruses. Journal of Virology 40:963–970
    [Google Scholar]
  13. Roller R., Stocring C., Smajda-Joffe F., Ostertag W. 1984; Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature sensitive mutants. Journal of Virology 50:717–724
    [Google Scholar]
  14. Le Bousse-Rerdiles M. C., Smajda-Joffe F., Rlein B., Caillou B., Jasmin C. 1980; Study of a virus-induced myeloproliferative syndrome associated with tumour formation in mice. European Journal of Cancer 16:43–51
    [Google Scholar]
  15. LE Bousse-Rerdiles M. C., Dumenil D., Smadja-Joffe F., Bertoli A. M., Degiorgis V., Auger-Buendia M. A., Tavitian A., Jasmin C. 1985; Study of the 78A1 isolate of Moloney murine sarcoma virus. II. Haematopoietic tropism and tumourigenicity. Journal of General Virology 66:2415–2421
    [Google Scholar]
  16. Lowy D. R., Rands E., Scolnick E. M. 1978; Helper-independent transformation by unintegrated Harvey sarcoma virus. Journal of Virology 26:291–298
    [Google Scholar]
  17. Lusky M., Botchan M. 1981; Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature, London 293:75–81
    [Google Scholar]
  18. Maniatis T., Fritsch E. F., Sambroor J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Moloney J. B. 1966; A virus-induced rhabdomyosarcoma of mice. National Cancer Institute Monograph 22:139–142
    [Google Scholar]
  20. Oskarsson M., Mcclements W. L., Blair D. G., Maizel J. V., Vande Woude G. F. 1980; Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus. Science 207:1222–1224
    [Google Scholar]
  21. Pragnell I. B., Fusco C., Arbuthnott C., Smajda-Joffe F., Rlein B., Jasmin C., Ostertag W. 1981; Analysis of the myeloproliferative sarcoma virus genome: limited changes in the prototype lead to altered target cell specificity. Journal of Virology 38:952–957
    [Google Scholar]
  22. Reddy E. P., Smith M. J., Aaronson S. A. 1981; Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome. Science 214:445–450
    [Google Scholar]
  23. Seif R., Cuzin F. 1977; Temperature sensitive growth regulation in one type of transformed rat cells induced by the ts mutant of polyoma virus. Journal of Virology 24:721–728
    [Google Scholar]
  24. Stacey A., Arbuthnott C., Roller R., Coggins L., Ostertag W. 1984; Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis. Journal of Virology 50:725–732
    [Google Scholar]
  25. Vanbeveren C., Van Straaten F., Galleshaw J. A., Verma I. M. 1981; Nucleotide sequence of the genome of a murine sarcoma virus. Cell 27:97–108
    [Google Scholar]
  26. Van Der Hoorn F. A., Hulsebos E., Berns A. J. M., Bloemers H. P. J. 1982; Molecularly cloned c-mos (rat) is biologically active. EMBO Journal 1:1313–1317
    [Google Scholar]
  27. Vande Woude G. F., Osrarsson M. K., Enquist L. W., Nomura S., Sullivan M., Fischinger P. T. 1979; Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda. Proceedings of the National Academy of Sciences, U. S. A 76:4464–4468
    [Google Scholar]
  28. Verma I. M., Mcrennett M. A. 1978; Genome organization of RNA tumor viruses. II. Physical maps of in vitro synthesized Moloney murine leukemia virus double-stranded DNA by restriction endonuclease. Journal of Virology 26:630–640
    [Google Scholar]
  29. Verma I. M., Lai M. H. T., Bosselman R., Mcrennett M. A., Fan H., Berns A. 1980; Molecular cloning of unintegrated Moloney mouse sarcoma virus DNA in bacteriophage X. Proceedings of the National Academy of Sciences, U. S. A 77:1773–1777
    [Google Scholar]
  30. Williams B. G., Blattner F. R. 1980; Bacteriophage vectors for DNA cloning. In Genetic Engineering vol 2: p 201 Edited by Setlow J. K., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  31. Wood T. G., Mcgeady M. L., Blair D. G., Vande Woude G. F. 1983; Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions. Journal of Virology 46:726–736
    [Google Scholar]
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