The nucleotide sequence of a genomic cDNA clone corresponding to the 5′ terminal domain of mRNA D of the Beaudette strain of infectious bronchitis virus (IBV) has been determined. This region contains three open reading frames which predict polypeptides of molecular weights 6700 s(6.7K), 7.4K and 12.4K. The predicted 12.4K polypeptide has a codon usage very similar to that predicted for the products of the IBV nucleocapsid, membrane and spike genes. The sequence also predicts a hydrophobic, potentially membrane-anchoring, region in the N terminal half of the 12.4K polypeptide, and a hydrophilic C terminus.
BankierA.,
BarrellB. G.1983; Shotgun DNA sequencing. In Techniques in the Life Sciences(Biochemistry), col. B5: Techniques in Nucleic Acid Biochemistry pp. B508 1–34 Edited by
FlavellR. A.
Ireland: Elsevier;
BinnsM. M.,
BoursnellM. E. G.,
CavanaghD.,
PappinD. J. C.,
BrownT. D. K.1985; Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. Journal of General Virology 66:719–726
BoursnellM. E. G.,
BinnsM. M.,
FouldsI. J.,
BrownT. D. K.1985; Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus. Journal of General Virology 66:573–580
BrownT. D. K.,
BoursnellM. E. G.1984; Avian infectious bronchitis virus genomic RNA contains sequence homologies at the intergenic boundaries. Virus Research 1:15–24
BrownT. D. K.,
BoursnellM. E. G.,
BinnsM. M.1984; A leader sequence is present on mRNA A of avian infectious bronchitis virus. Journal of General Virology 65:1437–1442
GeilhausenH. E.,
LigonF. B.,
LukertP. D.1972; The pathogenesis of virulent and avirulent avian infectious bronchitis virus. Archie fur die gesamte Virusforschung 40:285–290
GoadW. B.,
KanehisaM.i982; Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries. Nucleic Acids Research 10:247–263
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
SkinnerM. A.,
EbnerD.,
SiddellS. G.1985; Coronavirus MHV-JHM mRNA 5 has a sequence arrangement which potentially allows translation of a second, downstream open reading frame. Journal of General Virology 66:581–592
SternD. F.,
KennedyS. I. T.1980a; Coronavirus multiplication strategy. 1 Identification and characterisation of virus-specified RNA. Journal of Virology 34:665–674
SternD. F.,
KennedyS. I. T.1980b; Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. Journal of Virology 36:440–449
SternD. F.,
SeftonB. M.1984; Coronavirus multiplication: the locations of genes for the virion proteins on the avian infectious bronchitis virus genome. Journal of Virology 50:22–29