@article{mbs:/content/journal/jgv/10.1099/0022-1317-66-10-2089, author = "Diaco, R. and Hill, J. H. and Hill, E. K. and Tachibana, H. and Durand, D. P.", title = "Monoclonal Antibody-based Biotin-Avidin ELISA for the Detection of Soybean Mosaic Virus in Soybean Seeds", journal= "Journal of General Virology", year = "1985", volume = "66", number = "10", pages = "2089-2094", doi = "https://doi.org/10.1099/0022-1317-66-10-2089", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-10-2089", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "biotin-avidin", keywords = "seeds", keywords = "ELISA", keywords = "SMV", abstract = "SUMMARY Two monoclonal antibodies (M-Ab) specific for different epitopes on particles of soybean mosaic virus (SMV) were used in a double-antibody sandwich ELISA (M-Ab ELISA). The non-isotopic immunoassay, which used a biotinylated second antibody and an avidin-alkaline phosphatase detection system to detect SMV in soybean seed extracts, was compared with a polyclonal antibody-based solid-phase radioimmunoassay (SPRIA). m-Ab ELISA detected less than 10 ng of SMV/ml and was more sensitive than the SPRIA which detected 25 ng SMV/ml. Furthermore, m-Ab ELISA required less than 36 h for seed sample assays and only 5.5 h for assays involving purified virus, whereas SPRIA required 3 or 4 days. When seeds from 33 field plots, in which 0%, 30% and 50% of the soybean plants had been inoculated with SMV, were assayed by both systems, results of the two tests correlated for 31 of 33 (94%) seed samples. This suggests that dual-site biotin-avidin m-Ab ELISA systems have potential utility for routine screening of seed samples.", }