1887

Abstract

SUMMARY

When infected with Friend virus complex, the bone marrow cells of susceptible mice form large colonies (bursts) of erythroblasts after 5 days of culture in semi-solid medium. This virus-induced burst growth occurs without the addition of erythropoietin (EP) which is normally required for erythroid progenitor growth . Erythroid progenitor cells from C57BL/6 mice infected with Friend virus are resistant to virus-induced burst growth, while cells from the B6. S mouse strain, which is congenic with C57BL/6 but possesses the ‘Friend virus sensitivity’ alleles at the locus, are susceptible. This susceptibility of the B6. S cells demonstrates that virus-induced burst growth is regulated by the gene. Two mechanisms by which the locus could control virus resistance were analysed. The possible modulation of the erythroproliferative effect of the virus by soluble substances which either promote burst growth in the sensitive strains or inhibit growth in the resistant strain was examined. Also, the possible restriction of virus infection or replication in resistant ( ) haemopoietic cells was investigated. In a variety of experimental conditions designed to test the effects of soluble growth promoters on bone marrow cells infected , the resistance of C57BL/6 cells to erythroid burst formation could not be overcome. Neither could resistance be transferred to co-cultured sensitive cells by any soluble substances produced in culture by C57BL/6 cells. Use of haemopoietic cells from C57BL/6 animals in various physiological states of haemopoiesis also did not overcome the resistance to virus-induced burst growth. Quantification of several parameters of viral replication in whole marrow cultures or in erythroblasts from bursts of the sensitive and resistant congenic mouse strains showed that haemopoietic cells of both strains support virus growth equally well. These data suggest that -mediated resistance to the erythroproliferative effect of Friend virus infection is an inherent property of an erythroid progenitor target cell and is not determined by external factors. The resistance is also not due to restriction of virus replication.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-1-83
1985-01-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/1/JV0660010083.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-1-83&mimeType=html&fmt=ahah

References

  1. Alberto B. P., Callahan L. F., Pincus T. 1982; Evidence that retrovirus expression in mouse spleen cells results from B cell differentiation. Journal of Immunology 129:2768–2772
    [Google Scholar]
  2. Alter B. P., Goff S. C. 1980; Hemoglobin switching: a cellular model. Science 207:647–649
    [Google Scholar]
  3. Axelrad A. A., Steeves R. A. 1964; Assay for Friend leukemia virus: rapid quantitative method based on enumeration of macroscopic spleen foci. Virology 24:513–518
    [Google Scholar]
  4. Axelrad A. A., Ware M., Van Der Gaag H. C. 1972; Host cell susceptibility and resistance to murine leukemia viruses and their genetic control. In RNA Viruses and Host Genome in Oncogenesis pp 239–254 Edited by Emmelot P., Bentvelzan P. Amsterdam: North-Holland;
    [Google Scholar]
  5. Axelrad A. A., Mcleod D. L., Shreeve M. M., Heath D. S. 1973; Properties of cells that produce erythrocytic colonies in vitro. In Proceedings of the Second International Workshop on Hemopoiesis in Culture pp 226–234 Edited by Robinson W. A. Washington, D C.: DHEW Publication NIH 74-205;
    [Google Scholar]
  6. Axelrad A. A., Croizat H., Eskinazi D. 1981; A washable macromolecule from Fv- 2rr marrow cells negatively regulates DNA synthesis in erythropoietic progenitor cells BFU-E. Cell 26:233–244
    [Google Scholar]
  7. Coffin J. 1982; Structure of the retroviral genome. In RNA Tumor Viruses: Molecular Biology of Tumor Viruses 2nd edn. pp 343–344 Edited by Weiss R., Teich N., Varmus H., Coffin J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Evans L. H., Duesberg P. H., Scolnick E. M. 1980; Replication of spleen focus-forming Friend virus in fibroblasts from C57BL mice that are genetically resistant to spleen focus formation. Virology 101:534–539
    [Google Scholar]
  9. Hankins W. D., Luna J. 1982; Genetic restriction (Fv-1 and Fv-2) of in vitro erythroid transformation by Friend polycythemia virus. In Advances in Comparative Leukemia Research 1981 pp 107–109 Edited by Yohn D. S., Blakeslee J. R. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  10. Hankins W. D., Troxler D. 1980; Polycythemia- and anemia-inducing erythroleukemia viruses exhibit differential erythroid transforming effects in vitro. Cell 22:693–699
    [Google Scholar]
  11. Hankins W. D., Kost T. A., Koury M. I., Krantz S. B. 1978; Erythroid bursts produced by Friend leukaemia virus in vitro. Nature, London 276:506–508
    [Google Scholar]
  12. Hankins W. D., Kost T. A., Koury M. J., Krantz S. B. 1979; An in vitro system for erythroid transformation by Friend leukemia virus. In Experimental Hematology Today 1979: pp 89–97 Edited by Baum S. S., Ledney G. D. New York: Springer-Verlag;
    [Google Scholar]
  13. Iscove N. N. 1978; Erythropoietin independent stimulation of early erythropoiesis in adult marrow cultures by conditioned media from lectin stimulated mouse spleen cells. In Hematopoietic Celt Differentiation pp 37–52 Edited by Golde D. W., Cline M. J., Metcalf D., Fox C. F. New York: Academic Press;
    [Google Scholar]
  14. Johnson G. R., Metcalf D. 1977; Pure and mixed erythroid colony formation in vitro stimulated by spleen conditioned medium with no detectable erythropoietin. Proceedings of the National Academy of Sciences, U.S.A 74:3879–3882
    [Google Scholar]
  15. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  16. Kost T. A., Koury M. J., Hankins W. D., Krantz S. B. 1979; Target cells for Friend virus-induced erythroid bursts in vitro. Cell 18:145–152
    [Google Scholar]
  17. Kost T. A., Koury M. J., Krantz S. B. 1981; Mature erythroid burst-forming units are target cells for Friend virus-induced erythroid bursts. Virology 108:309–317
    [Google Scholar]
  18. Koury M. J., Krantz S. B. 1982; Velocity sedimentation profiles of normal and regenerating primitive erythroid burst-forming units: relation to phase of cell cycle and growth in vitro. Cell and Tissue Kinetics 15:59–67
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Mcmahon Y., Hankins W. D. 1980; Methylcellulose cultures clotted and stained for permanent record. Experimental Hematology 8:1081–1085
    [Google Scholar]
  21. Odaka T. 1967; Inheritance of susceptibility to Friend mouse leukemia virus. IV. Persistence of Friend leukemia virus in C57BL/6 mice. Japanese Journal of Experimental Medicine 37:71–72
    [Google Scholar]
  22. Odaka T. 1970; Inheritance of susceptibility to Friend mouse leukemia virus. VII. Establishment of a resistant strain. International Journal of Cancer 6:18–23
    [Google Scholar]
  23. Oliff A. L., Hager G. L., Chang E. H., Scolnick E. M., Chan H. W., Lowy D. R. 1980; Transfection of molecularly cloned Friend murine leukemia virus DNA yields a highly leukemogenic helper-independent type C virus. Journal of Virology 33:475–486
    [Google Scholar]
  24. Rave N., Crkvenjakov R., Boedtker H. 1979; Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde-agarose gels. Nucleic Acids Research 6:3559–3567
    [Google Scholar]
  25. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  26. Rowe W. P., Pugh W., Hartley J. W. 1970; Plaque assay technique for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  27. Scherrer K., Darnell J. E. 1962; Sedimentation characteristics of rapidly labeled RNA from HELA cells. Biochemical and Biophysical Research Communications 1:486–490
    [Google Scholar]
  28. Silver J., Teich N. 1981; Expression of resistance to Friend virus-stimulated erythropoiesis in bone marrow chimeras containing Fv- 2rr and Fv- 2SS bone marrow. Journal of Experimental Medicine 154:126–137
    [Google Scholar]
  29. Steeves R. A., Lilly F., Steinheider G., Blank K. 1978; The effect of the Fv- 2rr gene on spleen focus-forming virus and on embryonic development. In Differentiation of Normal and Neoplastic Hemopoietic Cells pp 591–600 Edited by Clarkson B., Marks P. A., Till J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Suzuki S., Axelrad A. A. 1980; Fv-2 locus controls the proportion of erythropoietic progenitor cells (BFU-E) synthesizing DNA in normal mice. Cell 19:225–236
    [Google Scholar]
  31. Tambourin P., Wendling F. 1971; Malignant transformation and erythroid differentiation by polycythaemia-inducing Friend virus. Nature New Biology 234:230–233
    [Google Scholar]
  32. Tambourin P. E., Gallien-Lartigue O., Wendling F., Huaulme D. H. 1973; Erythrocyte production in mice infected by the polycythaemia-inducing Friend virus or by the anaemia-inducing Friend virus. British Journal of Haematology 24:511–524
    [Google Scholar]
  33. Teich N. M., Dexter T. M. 1979; Interactions between murine leukemia viruses and differentiating hemopoietic cells. In Oncogenic Viruses and Host Cell Genes pp 263 Edited by Ikawa Y., Odaka T. New York: Academic Press;
    [Google Scholar]
  34. Teich N., Wyke J., Mak T., Bernstein A., Hardy W. 1982; Pathogenesis of retrovirus-induced disease. In RNA Tumor Viruses: Molecular Biology of Tumor Viruses 2nd edn pp 871–880 Edited by Weiss R., Teich N., Varmus H., Coffin J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  36. Troxler D. H., Ruscetti S. K., Scolnick E. M. 1980; The molecular biology of Friend virus. Biochimica et biophysica acta 605:305–324
    [Google Scholar]
  37. Witte O. N., Rosenberg N., Baltimore D. 1979; Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus-encoded Pl 20 protein at the cell surface. Journal of Virology 31:777–784
    [Google Scholar]
  38. Yoosook C., Steeves R., Lilly F. 1980; Fr-Z mediated resistance of mouse bone marrow cells to Friend spleen focus-forming virus infection. International Journal of Cancer 26:101–106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-1-83
Loading
/content/journal/jgv/10.1099/0022-1317-66-1-83
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error